公开(公告)号：US20240360423A1

公开(公告)日：2024-10-31

申请号：US18648199

申请日：2024-04-26

Applicant: Genzyme Corporation

Inventor： Srinivas Nellimarla ,  Kevin Victor Chen ,  Jeong Lee ,  Carlos Benitez ,  Ying Liu

IPC: C12N7/00 ,  C12N15/86

CPC classification number: C12N7/00 ,  C12N15/86 ,  C12N2750/14143 ,  C12N2750/14151

Abstract: This disclosure relates to methods and compositions for scaling up production of rAAV by cell cultures. In some aspects, the present disclosure relates to methods of transferring a plurality of transfection complexes to a bioreactor. Also provided are methods of improving production of recombinant adeno-associated virus (rAAV) particles by adding a second medium to the cell culture after transfection, thereby improving rAAV particle yield and reducing the amount of time needed to produce high levels of rAAV particles.