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公开(公告)号：US20250027031A1
公开(公告)日：2025-01-23
申请号：US18823894
申请日：2024-09-04
Applicant: Genzyme Corporation
Inventor： Jiuyi Lu , Jason Walther , Jonathan Wang , Kevin Victor Chen
Abstract: The disclosure provides a filtration system for a cell culture apparatus and a method of cell culture. The filtration system comprises a bioreactor vessel and two or more alternating tangential flow (ATF) filters connected in parallel. A failure in either filter is detected by an in-line sensor, and an automated response system functions to sequester the malfunctioning filter by stopping the flow of liquid media through the filter. Media flow through the remaining operable filters can be increased so that the rate of perfusion through the bioreactor remains relatively unchanged. Such a system may prevent issues that arise from ATF filter failures in conventional perfusion bioreactors, thereby improving the long-term viability of cell cultures.
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公开(公告)号：US12110483B2
公开(公告)日：2024-10-08
申请号：US18308870
申请日：2023-04-28
Applicant: Genzyme Corporation
Inventor： Jiuyi Lu , Jason Walther , Jonathan Wang , Kevin Victor Chen
CPC classification number: C12M41/48 , B01D15/1807 , B01D61/18 , B01D61/22 , C12M29/04 , C12M29/10 , C12M41/40 , B01D2311/2626 , B01D2311/2688 , B01D2313/243 , B01D2315/10 , B01D2317/04 , B01D2317/06
Abstract: The disclosure provides a filtration system for a cell culture apparatus and a method of cell culture. The filtration system comprises a bioreactor vessel and two or more alternating tangential flow (ATF) filters connected in parallel. A failure in either filter is detected by an in-line sensor, and an automated response system functions to sequester the malfunctioning filter by stopping the flow of liquid media through the filter. Media flow through the remaining operable filters can be increased so that the rate of perfusion through the bioreactor remains relatively unchanged. Such a system may prevent issues that arise from ATF filter failures in conventional perfusion bioreactors, thereby improving the long-term viability of cell cultures.
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Patent Agency Ranking
公开(公告)号：US11434464B2
公开(公告)日：2022-09-06
申请号：US17524269
申请日：2021-11-11
Applicant: Genzyme Corporation
Inventor： Jiuyi Lu , Jason Walther , Jonathan Wang , Kevin Victor Chen
Abstract: The disclosure provides a filtration system for a cell culture apparatus and a method of cell culture. The filtration system comprises a bioreactor vessel and two or more alternating tangential flow (ATF) filters connected in parallel. A failure in either filter is detected by an in-line sensor, and an automated response system functions to sequester the malfunctioning filter by stopping the flow of liquid media through the filter. Media flow through the remaining operable filters can be increased so that the rate of perfusion through the bioreactor remains relatively unchanged. Such a system may prevent issues that arise from ATF filter failures in conventional perfusion bioreactors, thereby improving the long-term viability of cell cultures.
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公开(公告)号：US20220073861A1
公开(公告)日：2022-03-10
申请号：US17524269
申请日：2021-11-11
Applicant: Genzyme Corporation
Inventor： Jiuyi Lu , Jason Walther , Jonathan Wang , Kevin Victor Chen
Abstract: The disclosure provides a filtration system for a cell culture apparatus and a method of cell culture. The filtration system comprises a bioreactor vessel and two or more alternating tangential flow (ATF) filters connected in parallel. A failure in either filter is detected by an in-line sensor, and an automated response system functions to sequester the malfunctioning filter by stopping the flow of liquid media through the filter. Media flow through the remaining operable filters can be increased so that the rate of perfusion through the bioreactor remains relatively unchanged. Such a system may prevent issues that arise from ATF filter failures in conventional perfusion bioreactors, thereby improving the long-term viability of cell cultures.
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公开(公告)号：US20240360423A1
公开(公告)日：2024-10-31
申请号：US18648199
申请日：2024-04-26
Applicant: Genzyme Corporation
Inventor： Srinivas Nellimarla , Kevin Victor Chen , Jeong Lee , Carlos Benitez , Ying Liu
CPC classification number: C12N7/00 , C12N15/86 , C12N2750/14143 , C12N2750/14151
Abstract: This disclosure relates to methods and compositions for scaling up production of rAAV by cell cultures. In some aspects, the present disclosure relates to methods of transferring a plurality of transfection complexes to a bioreactor. Also provided are methods of improving production of recombinant adeno-associated virus (rAAV) particles by adding a second medium to the cell culture after transfection, thereby improving rAAV particle yield and reducing the amount of time needed to produce high levels of rAAV particles.
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公开(公告)号：US20230340396A1
公开(公告)日：2023-10-26
申请号：US18308870
申请日：2023-04-28
Applicant: Genzyme Corporation
Inventor： Jiuyi Lu , Jason Walther , Jonathan Wang , Kevin Victor Chen
CPC classification number: C12M41/48 , B01D15/1807 , B01D61/18 , B01D61/22 , C12M29/04 , C12M29/10 , C12M41/40 , B01D2311/2626 , B01D2311/2688 , B01D2313/243 , B01D2315/10 , B01D2317/04 , B01D2317/06
Abstract: The disclosure provides a filtration system for a cell culture apparatus and a method of cell culture. The filtration system comprises a bioreactor vessel and two or more alternating tangential flow (ATF) filters connected in parallel. A failure in either filter is detected by an in-line sensor, and an automated response system functions to sequester the malfunctioning filter by stopping the flow of liquid media through the filter. Media flow through the remaining operable filters can be increased so that the rate of perfusion through the bioreactor remains relatively unchanged. Such a system may prevent issues that arise from ATF filter failures in conventional perfusion bioreactors, thereby improving the long-term viability of cell cultures.
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公开(公告)号：US20190338238A1
公开(公告)日：2019-11-07
申请号：US16403161
申请日：2019-05-03
Applicant: Genzyme Corporation
Inventor： Jiuyi Lu , Jason Walther , Jonathan Wang , Kevin Victor Chen
Abstract: The disclosure provides a filtration system for a cell culture apparatus and a method of cell culture. The filtration system comprises a bioreactor vessel and two or more alternating tangential flow (ATF) filters connected in parallel. A failure in either filter is detected by an in-line sensor, and an automated response system functions to sequester the malfunctioning filter by stopping the flow of liquid media through the filter. Media flow through the remaining operable filters can be increased so that the rate of perfusion through the bioreactor remains relatively unchanged. Such a system may prevent issues that arise from ATF filter failures in conventional perfusion bioreactors, thereby improving the long-term viability of cell cultures.
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