公开(公告)号：US20240287451A1

公开(公告)日：2024-08-29

申请号：US18656253

申请日：2024-05-06

Applicant: Genzyme Corporation

Inventor： Agata Villiger-Oberbek ,  Jianguo Yang ,  Yang Yang ,  Gabrielle Lorenzo

IPC: C12N5/071 ,  C12M1/00 ,  C12M1/32 ,  C12M3/04 ,  C12P21/00

CPC classification number: C12N5/0602 ,  C12M23/12 ,  C12M27/10 ,  C12M29/10 ,  C12P21/00 ,  C12N2510/02 ,  C12N2527/00

Abstract: Provided herein are methods of culturing a mammalian cell and various methods that utilize these culturing methods. Also provided are multi-well cell culture plates, e.g., for use in perfusion culturing methods.

公开(公告)号：US20200255798A1

公开(公告)日：2020-08-13

申请号：US16807057

申请日：2020-03-02

Applicant: Genzyme Corporation

Inventor： Jianguo Yang ,  Yang Yang ,  Jennifer Tengtrakool ,  Weichang Zhou

IPC: C12N5/00

Abstract: Provided herein are methods of perfusion culturing an adherent mammalian cell using a shake flask and a plurality of microcarriers, and various methods that utilize these culturing methods.

公开(公告)号：US10577583B2

公开(公告)日：2020-03-03

申请号：US15874645

申请日：2018-01-18

Applicant: Genzyme Corporation

Inventor： Jianguo Yang ,  Yang Yang ,  Jennifer Tengtrakool ,  Weichang Zhou

IPC: C12P21/00 ,  C12N5/00

Abstract: Provided herein are methods of perfusion culturing an adherent mammalian cell using a shake flask and a plurality of microcarriers, and various methods that utilize these culturing methods.

公开(公告)号：US10421949B2

公开(公告)日：2019-09-24

申请号：US14769783

申请日：2014-02-21

Applicant: Genzyme Corporation

Inventor： Jianguo Yang ,  Yang Yang

IPC: C12N5/071 ,  C12N5/00 ,  C12P21/00

Abstract: Provided herein are methods of culturing an adherent mammalian cell in a shake tube using a plurality of microcarriers and batch re-feed perfusion, and various methods that utilize these culturing methods.

公开(公告)号：US09909101B2

公开(公告)日：2018-03-06

申请号：US14769772

申请日：2014-02-21

Applicant: Genzyme Corporation

Inventor： Jianguo Yang ,  Yang Yang ,  Jennifer Tengtrakool ,  Weichang Zhou

IPC: C12N5/07 ,  C12N5/00

CPC classification number: C12N5/0075 ,  C12N2510/02 ,  C12N2527/00 ,  C12N2531/00

Abstract: Provided herein are methods of perfusion culturing an adherent mammalian cell using a shake flask and a plurality of microcarriers, and various methods that utilize these culturing methods.

公开(公告)号：US20140154726A1

公开(公告)日：2014-06-05

申请号：US14061657

申请日：2013-10-23

Applicant: Genzyme Corporation

Inventor： Jianguo Yang ,  Agata Villiger-Oberbek ,  Yang Yang

IPC: C12P21/00 ,  C12N9/40

Abstract: Provided herein are improved methods of culturing a mammalian cell in a conical container, and methods that utilize these culturing methods.

Abstract translation: 本文提供了在锥形容器中培养哺乳动物细胞的改进方法，以及利用这些培养方法的方法。

公开(公告)号：US12006510B2

公开(公告)日：2024-06-11

申请号：US17358889

申请日：2021-06-25

Applicant: Genzyme Corporation

Inventor： Agata Villiger-Oberbek ,  Jianguo Yang ,  Yang Yang ,  Gabrielle Lorenzo

IPC: C12N5/071 ,  C12M1/00 ,  C12M1/32 ,  C12M3/04 ,  C12P21/00

CPC classification number: C12N5/0602 ,  C12M23/12 ,  C12M27/10 ,  C12M29/10 ,  C12P21/00 ,  C12N2510/02 ,  C12N2527/00

Abstract: Provided herein are methods of culturing a mammalian cell and various methods that utilize these culturing methods. Also provided are multi-well cell culture plates, e.g., for use in perfusion culturing methods.

公开(公告)号：US20210324328A1

公开(公告)日：2021-10-21

申请号：US17358889

申请日：2021-06-25

Applicant: Genzyme Corporation

Inventor： Agata Villiger-Oberbek ,  Jianguo Yang ,  Yang Yang ,  Gabrielle Lorenzo

IPC: C12N5/071 ,  C12P21/00 ,  C12M1/00 ,  C12M1/32 ,  C12M3/04

Abstract: Provided herein are methods of culturing a mammalian cell and various methods that utilize these culturing methods. Also provided are multi-well cell culture plates, e.g., for use in perfusion culturing methods.

公开(公告)号：US20180148684A1

公开(公告)日：2018-05-31

申请号：US15874645

申请日：2018-01-18

Applicant: Genzyme Corporation

Inventor： Jianguo Yang ,  Yang Yang ,  Jennifer Tengtrakool ,  Weichang Zhou

IPC: C12N5/00

Abstract: Provided herein are methods of perfusion culturing an adherent mammalian cell using a shake flask and a plurality of microcarriers, and various methods that utilize these culturing methods.

公开(公告)号：US20160017280A1

公开(公告)日：2016-01-21

申请号：US14732325

申请日：2015-06-05

Applicant: Genzyme Corporation

Inventor： Agata Villiger-Oberbek ,  Jianguo Yang ,  Yang Yang ,  Gabrielle Lorenzo

IPC: C12N5/071 ,  C12P21/00

CPC classification number: C12N5/0602 ,  C12M23/12 ,  C12M27/10 ,  C12M29/10 ,  C12N2510/02 ,  C12N2527/00 ,  C12P21/00

Abstract: Provided herein are methods of culturing a mammalian cell and various methods that utilize these culturing methods. Also provided are multi-well cell culture plates, e.g., for use in perfusion culturing methods.

Abstract translation: 本文提供培养哺乳动物细胞的方法和利用这些培养方法的各种方法。 还提供了多孔细胞培养板，例如用于灌注培养方法。