公开(公告)号：US20100005541A1

公开(公告)日：2010-01-07

申请号：US12514676

申请日：2007-11-13

申请人： George Mather Sauer ,  Ralf Flachmann ,  Christel Renate Schopfer ,  Michael Leps ,  Hannia Bridg-Giannakopoulos

发明人： George Mather Sauer ,  Ralf Flachmann ,  Christel Renate Schopfer ,  Michael Leps ,  Hannia Bridg-Giannakopoulos

IPC分类号： C12N15/82 ,  C12N9/10 ,  C12N15/11 ,  A01H5/00

CPC分类号： C12N15/825 ,  A01H1/02 ,  A01H1/06 ,  A01H5/02 ,  A23K20/179 ,  A23K50/80 ,  C12N9/0069 ,  C12N9/1022 ,  C12N15/823 ,  C12P23/00

摘要： New ketolases for the production of ketocarotenoids in Tagetes Abstract A ketolase having a polypeptide sequence SEQ ID NO:6, a sequence derived from SEQ ID NO:6 by substitution, insertion or deletion of aminoacids, which has an identity of at least 60% with the respective sequence SEQ. ID. NO: 6 or a functionally equivalent fragment of the sequences mentioned above and processes for production of ketocarotenoids in Tagetes.

摘要翻译： 用于生产Tagetes中的酮类胡萝卜素的新型酮酶本文摘要具有多肽序列SEQ ID NO：6的酮糖酶，通过取代，插入或缺失氨基酸得到的序列SEQ ID NO：6，其同一性至少为60％ 相应的序列SEQ。 ID。 NO：6或上述序列的功能等同片段和用于生产Tagetes中的酮类胡萝卜素的方法。

公开(公告)号：US20120156718A1

公开(公告)日：2012-06-21

申请号：US13140218

申请日：2009-12-08

申请人： Ralf Flachmann ,  Irina Wenderoth ,  Christel Renate Schopter ,  Bettina Tschiersch ,  Hannia Bridg-Giannakopoulos ,  Michael Leps ,  Ute Linemann ,  George Mather Sauer

发明人： Ralf Flachmann ,  Irina Wenderoth ,  Christel Renate Schopter ,  Bettina Tschiersch ,  Hannia Bridg-Giannakopoulos ,  Michael Leps ,  Ute Linemann ,  George Mather Sauer

IPC分类号： C12P23/00 ,  C12N1/00 ,  C12N15/53 ,  A01H5/10 ,  A01H1/06 ,  C12N15/63 ,  A01H5/00

CPC分类号： C12N15/825 ,  C12N9/88

摘要： The present invention relates to optimized ketolase coding sequences, corresponding coding sequences and genetic constructs alone or in combination with beta-cyclase coding sequences, their use for the expression in plants, in particular in plants of the genus Tagetes, to such genetically modified plants, to a process for the preparation of carotenoid products by culturing the genetically modified plants. The present invention further relates to the expression of optimized ketolase coding sequences alone or in combination with beta-cyclase coding sequences under control of an Antirrhinum majus ANTHIRRHINUM FIDDLEHEAD (AFI) promoter, In addition the invention relates to the use of an AFI promoter for heterologous gene expression, preferably for flower-specific expression of genes in plants of the genus Tagetes, to the genetically modified plants of the genus Tagetes, and to a process for producing biosynthetic products by cultivating the genetically modified plants.

摘要翻译： 本发明涉及优化的酮醇酶编码序列，相应的编码序列和单独的或与β-环化酶编码序列组合的遗传构建体，它们在植物中，特别是在加泰米松属植物中的表达用于此类转基因植物， 涉及通过培养转基因植物来制备类胡萝卜素产物的方法。 本发明还涉及在金鱼草ANTHIRRININ FIDDHEAD（AFI）启动子的控制下单独或与β-环化酶编码序列组合的优化的酮醇酶编码序列的表达。此外，本发明涉及AFI启动子在异源 基因表达，优选用于花旗特异性表达的Tagetes植物中的基因，转基因植物的Tagetes，以及通过培养转基因植物生产生物合成产物的方法。