公开(公告)号：US4977085A

公开(公告)日：1990-12-11

申请号：US584619

申请日：1984-02-29

申请人： George Sprague ,  Ira Herskowitz ,  Jeremy Thorner ,  David Julius ,  Lindley Blair ,  Anthony Brake

发明人： George Sprague ,  Ira Herskowitz ,  Jeremy Thorner ,  David Julius ,  Lindley Blair ,  Anthony Brake

IPC分类号： C12N9/48 ,  C12N15/81

CPC分类号： C12N15/81 ,  C12N9/48

摘要： A method is provided for expressing the proteases dipeptidyl aminopeptidases A and B which are useful for processing precursor proteins. Genes controlling these proteases may be insdrted into appropriate vectors and transformed into cultures. The proteases may either be utilized to process precursor proteins in vivo or may be extracted from cultures and used to process precursor proteins in vitro.

摘要翻译： 提供了用于表达可用于加工前体蛋白质的蛋白酶二肽基氨基肽酶A和B的方法。 控制这些蛋白酶的基因可以被插入适当的载体并转化成培养物。 蛋白酶可以用于体内处理前体蛋白质，也可以从培养物中提取，并用于体外处理前体蛋白质。

公开(公告)号：US5104795A

公开(公告)日：1992-04-14

申请号：US480003

申请日：1990-02-14

申请人： Jar-How Lee ,  Lindley Blair ,  Arnold Horwitz ,  Raju K. Koduri

发明人： Jar-How Lee ,  Lindley Blair ,  Arnold Horwitz ,  Raju K. Koduri

IPC分类号： C07K14/43 ,  C12N15/62 ,  C12N15/69 ,  C12N15/81

CPC分类号： C07K14/43 ,  C12N15/625 ,  C12N15/69 ,  C12N15/81 ,  C07K2319/02 ,  C07K2319/036

摘要： A shortened PGK promoter ranging from 165 to 404 base pairs of the Saccharomyces PGK promoter is taught. The promoter is operably linked to heterologous genes and improves the expression thereof relative to the full length PGK promoter.

摘要翻译： 教导了来自酿酒酵母PGK启动子的165至404碱基对的缩短的PGK启动子。 启动子可操作地连接到异源基因并相对于全长PGK启动子改进其表达。

公开(公告)号：US08512953B2

公开(公告)日：2013-08-20

申请号：US10253967

申请日：2002-09-24

申请人： Katsuyuki Saito ,  Jar-How Lee ,  Lindley Blair

发明人： Katsuyuki Saito ,  Jar-How Lee ,  Lindley Blair

IPC分类号： C12Q1/68 ,  C07H21/04

CPC分类号： C12Q1/6813 ,  C12Q1/6827 ,  C12Q1/6881 ,  C12Q2600/156 ,  C12Q2535/131 ,  C12Q2525/197

摘要： The invention provides methods for detecting target nucleic acid sequences with diagnostic probes including first and second probe regions that are substantially complementary to first and second target regions respectively on a target nucleic acid strand wherein the first probe region is located 5′ to the second probe region. The first probe region is substantially complementary to the first target region, on the target nucleic acid strand, which also includes a second target region, wherein when said first target region is contiguous with the second target region on the target nucleic acid strand, then the first and second probe regions on the diagnostic probe are separated by a spacer region of nucleic acid.

公开(公告)号：US06207379B1

公开(公告)日：2001-03-27

申请号：US09151465

申请日：1998-09-11

申请人： Jar-How Lee ,  Lindley Blair

发明人： Jar-How Lee ,  Lindley Blair

IPC分类号： C12Q168

CPC分类号： C12Q1/6881 ,  C12Q1/6858 ,  C12Q2600/156 ,  C12Q2537/143

摘要： The invention provides methods for detecting target nucleic acid sequences with diagnostic primers including priming regions and probe regions which are complementary to target and reference regions respectively on a sample nucleic acid strand wherein the probe region is located 5′ to the priming region which is complementary to a reference nucleic acid sequence which is 3′ to the target nucleic acid sequence on the sample nucleic acid strand wherein when said reference nucleic acid sequence is contiguous with said target nucleic acid sequence on the sample nucleic acid strand then the priming region and probe region on the diagnostic probe are separated by a spacer region of nucleic acid.