公开(公告)号：US06444268B2

公开(公告)日：2002-09-03

申请号：US09897340

申请日：2001-07-02

申请人： Steven M. Lefkowitz ,  Geraldine Fulcrand ,  Douglas J. Dellinger ,  Charles Z. Hotz

发明人： Steven M. Lefkowitz ,  Geraldine Fulcrand ,  Douglas J. Dellinger ,  Charles Z. Hotz

IPC分类号： B05D136

CPC分类号： B82Y30/00 ,  B05D1/185 ,  B32B17/10688 ,  B82Y40/00 ,  G01N33/54353 ,  Y10T428/261 ,  Y10T428/31612 ,  Y10T428/31663 ,  Y10T428/31667

摘要： Low surface energy functionalized surfaces on solid supports are provided by treating a solid support having hydrophilic moieties on its surface with a derivatizing composition containing a mixture of silanes. A first silane provides the desired reduction in surface energy, while the second silane enables functionalization with molecular moieties of interest, such as small molecules, initial monomers to be used in the solid phase synthesis of oligomers, or intact oligomers. Molecular moieties of interest may be attached through cleavable sites. Derivatizing compositions for carrying out the surface functionalization process are provided as well.

摘要翻译： 通过用含有硅烷混合物的衍生组合物处理其表面上具有亲水部分的固体支持物来提供固体载体上的低表面能官能化表面。 第一硅烷提供所需的表面能降低，而第二硅烷能够与目的分子部分进行官能化，例如小分子，用于低聚物的固相合成的初始单体或完整低聚物。 感兴趣的分子部分可以通过可裂解位点附着。 还提供用于进行表面官能化过程的衍生组合物。

公开(公告)号：US06319674B1

公开(公告)日：2001-11-20

申请号：US09397527

申请日：1999-09-16

申请人： Geraldine Fulcrand ,  Douglas J. Dellinger ,  Steven M. Lefkowitz

发明人： Geraldine Fulcrand ,  Douglas J. Dellinger ,  Steven M. Lefkowitz

IPC分类号： G01N3353

CPC分类号： G01N33/54353

摘要： Methods are disclosed for immobilizing a substance to a surface. A surface is employed that comprises a linking group consisting of a first portion comprising a hydrocarbon chain, optionally substituted, and a second portion comprising an alkylene oxide or an alkylene imine wherein the alkylene is optionally substituted. One end of the first portion is attached to the surface and one end of the second portion is attached to the other end of the first portion chain by means of an amine or an oxy functionality. The second portion terminates in an amine or a hydroxy functionality. The surface is reacted with the substance to be immobilized under conditions for attachment of the substance to the surface by means of the linking group. Compositions of matter and reaction systems are also disclosed.

摘要翻译： 公开了将物质固定在表面上的方法。 使用表面，其包含由包含任选取代的烃链的第一部分和包含烯化氧或亚烷基亚胺的第二部分组成的连接基团，其中所述亚烷基是任选取代的。 第一部分的一端附接到表面，并且第二部分的一端通过胺或氧官能团连接到第一部分链的另一端。 第二部分终止于胺或羟基官能团。 在通过连接基团将物质附着到表面的条件下，表面与要固定的物质反应。 还公开了物质和反应体系的组成。

公开(公告)号：US06258454B1

公开(公告)日：2001-07-10

申请号：US09145015

申请日：1998-09-01

申请人： Steven M. Lefkowitz ,  Geraldine Fulcrand ,  Douglas J. Dellinger ,  Charles Z. Hotz

发明人： Steven M. Lefkowitz ,  Geraldine Fulcrand ,  Douglas J. Dellinger ,  Charles Z. Hotz

IPC分类号： B32B1706

CPC分类号： B82Y30/00 ,  B05D1/185 ,  B32B17/10688 ,  B82Y40/00 ,  G01N33/54353 ,  Y10T428/261 ,  Y10T428/31612 ,  Y10T428/31663 ,  Y10T428/31667

摘要： Low surface energy functionalized surfaces on solid supports are provided by treating a solid support having hydrophilic moieties on its surface with a derivatizing composition containing a mixture of silanes. A first silane provides the desired reduction in surface energy, while the second silane enables functionalization with molecular moieties of interest, such as small molecules, initial monomers to be used in the solid phase synthesis of oligomers, or intact oligomers. Molecular moieties of interest may be attached through cleavable sites. Derivatizing compositions for carrying out the surface functionalization process are provided as well.

公开(公告)号：US06743585B2

公开(公告)日：2004-06-01

申请号：US09981580

申请日：2001-10-17

申请人： Douglas J. Dellinger ,  Joel Myerson ,  Geraldine Fulcrand ,  Diane D. Ilsley

发明人： Douglas J. Dellinger ,  Joel Myerson ,  Geraldine Fulcrand ,  Diane D. Ilsley

IPC分类号： C12Q168

CPC分类号： A61K47/51 ,  Y10S436/829

摘要： Methods are disclosed for conjugating one moiety to another moiety. In the method the moieties are reacted with one another in a protic solvent. Reaction between the moieties and the protic solvent during the conjugating is negligible or reversible. A stable bond is formed between the moieties to produce a product that is not subject to &bgr;-elimination at elevated pH. Usually, one of the moieties comprises an unsaturation between two carbon atoms. One of the carbon atoms is or becomes an electrophile during the conjugating. The other of the moieties comprises a functionality reactive with the electrophile carbon atom to form a product that comprises the unsaturation. Compounds comprising both of the moieties as well as precursor molecules are also disclosed. Methods are also disclosed for determining an analyte in a sample employing compounds as described above.

摘要翻译： 公开了将一个部分与另一部分结合的方法。 在该方法中，部分在质子溶剂中彼此反应。 在缀合期间部分和质子溶剂之间的反应是可忽略的或可逆的。 在部分之间形成稳定的键，以产生在升高的pH下不经受β-消除的产物。 通常，其中一个部分包含两个碳原子之间的不饱和键。 在共轭过程中碳原子之一是或成为亲电子体。 另一个部分包含与亲电子体碳原子反应的官能团以形成包含不饱和键的产物。 还公开了包含两个部分以及前体分子的化合物。 还公开了使用如上所述的化合物来测定样品中的分析物的方法。