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公开(公告)号：US06743585B2
公开(公告)日：2004-06-01
申请号：US09981580
申请日：2001-10-17
IPC分类号： C12Q168
CPC分类号： A61K47/51 , Y10S436/829
摘要： Methods are disclosed for conjugating one moiety to another moiety. In the method the moieties are reacted with one another in a protic solvent. Reaction between the moieties and the protic solvent during the conjugating is negligible or reversible. A stable bond is formed between the moieties to produce a product that is not subject to &bgr;-elimination at elevated pH. Usually, one of the moieties comprises an unsaturation between two carbon atoms. One of the carbon atoms is or becomes an electrophile during the conjugating. The other of the moieties comprises a functionality reactive with the electrophile carbon atom to form a product that comprises the unsaturation. Compounds comprising both of the moieties as well as precursor molecules are also disclosed. Methods are also disclosed for determining an analyte in a sample employing compounds as described above.
摘要翻译： 公开了将一个部分与另一部分结合的方法。 在该方法中，部分在质子溶剂中彼此反应。 在缀合期间部分和质子溶剂之间的反应是可忽略的或可逆的。 在部分之间形成稳定的键，以产生在升高的pH下不经受β-消除的产物。 通常，其中一个部分包含两个碳原子之间的不饱和键。 在共轭过程中碳原子之一是或成为亲电子体。 另一个部分包含与亲电子体碳原子反应的官能团以形成包含不饱和键的产物。 还公开了包含两个部分以及前体分子的化合物。 还公开了使用如上所述的化合物来测定样品中的分析物的方法。
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公开(公告)号：US6103474A
公开(公告)日：2000-08-15
申请号：US183619
申请日：1998-10-30
CPC分类号： C12Q1/682
摘要： A signal amplification method for detecting a target nucleic acid analyte having a homopolymeric region and a target sequence includes steps of: contacting an analyte under hybridizing conditions with a multiplicity of reporter probes, each reporter probe including a signal region and an oligonucleotide sequence which is complementary to and capable of forming a stable hybrid with the analyte homopolymeric region to form an analyte:reporter probe hybrid; and forming an analyte:capture probe hybrid by contacting the analyte target sequence with a capture probe under hybridizing conditions. The analyte:reporter probe hybrid may formed prior to contacting the analyte target sequence with the capture probe, so the result of contacting the analyte target sequence with the capture probe results in formation of an analyte:reporter probe:capture probe complex. The analyte:capture probe hybrid may be immobilized on a solid generally planar surface in an array format. Multiple reporter probes may form triple helix structures for further signal enhancement. Multiply-labeled hairpin reporter probes may be employed according to the invention. Also, a kit for carrying out the invention includes one or more capture probes immobilized on a surface, reporter probes each having a signal region and a sequence for binding analyte homopolymer regions, and, optionally, reagents for addition of homopolymer regions to nucleic acid analytes and for carrying out the hybridization reaction.
摘要翻译： 用于检测具有均聚区和靶序列的靶核酸分析物的信号扩增方法包括以下步骤：在杂交条件下与多个报道探针接触分析物，每个报道探针包括信号区和互补的寡核苷酸序列 并且能够与分析物均聚区形成稳定的杂交体以形成分析物：报道探针杂交体; 并通过在杂交条件下将分析物靶序列与捕获探针接触来形成分析物：捕获探针杂交体。 在将分析物靶序列与捕获探针接触之前可以形成分析物：报告物探针杂交体，因此将分析物靶序列与捕获探针接触的结果导致分析物的形成：报道探针：捕获探针复合物。 分析物：捕获探针杂交体可以以阵列形式固定在固体大体上平坦的表面上。 多个报道探针可以形成用于进一步信号增强的三重螺旋结构。 根据本发明，可以使用多重标记的发夹报道探针。 此外，用于实施本发明的试剂盒包括固定在表面上的一种或多种捕获探针，各自具有信号区域的报道探针和用于结合分析物均聚物区域的序列，以及任选的用于向核酸分析物加入均聚物区域的试剂 并用于进行杂交反应。
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公开(公告)号：US07011949B2
公开(公告)日：2006-03-14
申请号：US10261405
申请日：2002-09-30
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6809 , C12Q2521/101 , C12Q2563/107 , C12Q2565/501
摘要： Methods and compositions for producing labeled probe nucleic acids from genomic nucleic acid template are provided. In the subject methods, a conserved coding consensus region primer is employed to enzymatically generate a select set of labeled probe nucleic acids corresponding to coding regions of genes from a genomic template via a primer extension protocol. The subject methods find use in a variety of different applications, and are particularly suited for use in the preparation of labeled probe nucleic acids for use in array based comparative genomic hybridization applications. Also provided are kits for use in practicing the subject methods.
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公开(公告)号：US20070148636A1
公开(公告)日：2007-06-28
申请号：US11317557
申请日：2005-12-23
申请人： Min-sun Song , Diane D. Ilsley
发明人： Min-sun Song , Diane D. Ilsley
CPC分类号： C12Q1/6844 , C12Q1/6841 , C12Q2521/101
摘要： Methods, compositions and kits are provided for labeling, copying and/or amplifying nucleic acids. The methods, compositions and kit can be used for a variety of applications, for example, genome-wide scanning applications such as CGH or location analysis.
摘要翻译： 提供了用于标记，复制和/或扩增核酸的方法，组合物和试剂盒。 方法，组合物和试剂盒可用于各种应用，例如全基因组扫描应用，如CGH或位置分析。
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