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公开(公告)号：US06743585B2
公开(公告)日：2004-06-01
申请号：US09981580
申请日：2001-10-17
IPC分类号： C12Q168
CPC分类号： A61K47/51 , Y10S436/829
摘要： Methods are disclosed for conjugating one moiety to another moiety. In the method the moieties are reacted with one another in a protic solvent. Reaction between the moieties and the protic solvent during the conjugating is negligible or reversible. A stable bond is formed between the moieties to produce a product that is not subject to &bgr;-elimination at elevated pH. Usually, one of the moieties comprises an unsaturation between two carbon atoms. One of the carbon atoms is or becomes an electrophile during the conjugating. The other of the moieties comprises a functionality reactive with the electrophile carbon atom to form a product that comprises the unsaturation. Compounds comprising both of the moieties as well as precursor molecules are also disclosed. Methods are also disclosed for determining an analyte in a sample employing compounds as described above.
摘要翻译： 公开了将一个部分与另一部分结合的方法。 在该方法中，部分在质子溶剂中彼此反应。 在缀合期间部分和质子溶剂之间的反应是可忽略的或可逆的。 在部分之间形成稳定的键，以产生在升高的pH下不经受β-消除的产物。 通常，其中一个部分包含两个碳原子之间的不饱和键。 在共轭过程中碳原子之一是或成为亲电子体。 另一个部分包含与亲电子体碳原子反应的官能团以形成包含不饱和键的产物。 还公开了包含两个部分以及前体分子的化合物。 还公开了使用如上所述的化合物来测定样品中的分析物的方法。
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公开(公告)号：US6103474A
公开(公告)日：2000-08-15
申请号：US183619
申请日：1998-10-30
CPC分类号： C12Q1/682
摘要： A signal amplification method for detecting a target nucleic acid analyte having a homopolymeric region and a target sequence includes steps of: contacting an analyte under hybridizing conditions with a multiplicity of reporter probes, each reporter probe including a signal region and an oligonucleotide sequence which is complementary to and capable of forming a stable hybrid with the analyte homopolymeric region to form an analyte:reporter probe hybrid; and forming an analyte:capture probe hybrid by contacting the analyte target sequence with a capture probe under hybridizing conditions. The analyte:reporter probe hybrid may formed prior to contacting the analyte target sequence with the capture probe, so the result of contacting the analyte target sequence with the capture probe results in formation of an analyte:reporter probe:capture probe complex. The analyte:capture probe hybrid may be immobilized on a solid generally planar surface in an array format. Multiple reporter probes may form triple helix structures for further signal enhancement. Multiply-labeled hairpin reporter probes may be employed according to the invention. Also, a kit for carrying out the invention includes one or more capture probes immobilized on a surface, reporter probes each having a signal region and a sequence for binding analyte homopolymer regions, and, optionally, reagents for addition of homopolymer regions to nucleic acid analytes and for carrying out the hybridization reaction.
摘要翻译： 用于检测具有均聚区和靶序列的靶核酸分析物的信号扩增方法包括以下步骤：在杂交条件下与多个报道探针接触分析物，每个报道探针包括信号区和互补的寡核苷酸序列 并且能够与分析物均聚区形成稳定的杂交体以形成分析物：报道探针杂交体; 并通过在杂交条件下将分析物靶序列与捕获探针接触来形成分析物：捕获探针杂交体。 在将分析物靶序列与捕获探针接触之前可以形成分析物：报告物探针杂交体，因此将分析物靶序列与捕获探针接触的结果导致分析物的形成：报道探针：捕获探针复合物。 分析物：捕获探针杂交体可以以阵列形式固定在固体大体上平坦的表面上。 多个报道探针可以形成用于进一步信号增强的三重螺旋结构。 根据本发明，可以使用多重标记的发夹报道探针。 此外，用于实施本发明的试剂盒包括固定在表面上的一种或多种捕获探针，各自具有信号区域的报道探针和用于结合分析物均聚物区域的序列，以及任选的用于向核酸分析物加入均聚物区域的试剂 并用于进行杂交反应。
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公开(公告)号：US06444268B2
公开(公告)日：2002-09-03
申请号：US09897340
申请日：2001-07-02
IPC分类号： B05D136
CPC分类号： B82Y30/00 , B05D1/185 , B32B17/10688 , B82Y40/00 , G01N33/54353 , Y10T428/261 , Y10T428/31612 , Y10T428/31663 , Y10T428/31667
摘要： Low surface energy functionalized surfaces on solid supports are provided by treating a solid support having hydrophilic moieties on its surface with a derivatizing composition containing a mixture of silanes. A first silane provides the desired reduction in surface energy, while the second silane enables functionalization with molecular moieties of interest, such as small molecules, initial monomers to be used in the solid phase synthesis of oligomers, or intact oligomers. Molecular moieties of interest may be attached through cleavable sites. Derivatizing compositions for carrying out the surface functionalization process are provided as well.
摘要翻译： 通过用含有硅烷混合物的衍生组合物处理其表面上具有亲水部分的固体支持物来提供固体载体上的低表面能官能化表面。 第一硅烷提供所需的表面能降低，而第二硅烷能够与目的分子部分进行官能化，例如小分子，用于低聚物的固相合成的初始单体或完整低聚物。 感兴趣的分子部分可以通过可裂解位点附着。 还提供用于进行表面官能化过程的衍生组合物。
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公开(公告)号：US06319674B1
公开(公告)日：2001-11-20
申请号：US09397527
申请日：1999-09-16
IPC分类号： G01N3353
CPC分类号： G01N33/54353
摘要： Methods are disclosed for immobilizing a substance to a surface. A surface is employed that comprises a linking group consisting of a first portion comprising a hydrocarbon chain, optionally substituted, and a second portion comprising an alkylene oxide or an alkylene imine wherein the alkylene is optionally substituted. One end of the first portion is attached to the surface and one end of the second portion is attached to the other end of the first portion chain by means of an amine or an oxy functionality. The second portion terminates in an amine or a hydroxy functionality. The surface is reacted with the substance to be immobilized under conditions for attachment of the substance to the surface by means of the linking group. Compositions of matter and reaction systems are also disclosed.
摘要翻译： 公开了将物质固定在表面上的方法。 使用表面，其包含由包含任选取代的烃链的第一部分和包含烯化氧或亚烷基亚胺的第二部分组成的连接基团，其中所述亚烷基是任选取代的。 第一部分的一端附接到表面，并且第二部分的一端通过胺或氧官能团连接到第一部分链的另一端。 第二部分终止于胺或羟基官能团。 在通过连接基团将物质附着到表面的条件下，表面与要固定的物质反应。 还公开了物质和反应体系的组成。
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公开(公告)号：US06258454B1
公开(公告)日：2001-07-10
申请号：US09145015
申请日：1998-09-01
IPC分类号： B32B1706
CPC分类号： B82Y30/00 , B05D1/185 , B32B17/10688 , B82Y40/00 , G01N33/54353 , Y10T428/261 , Y10T428/31612 , Y10T428/31663 , Y10T428/31667
摘要： Low surface energy functionalized surfaces on solid supports are provided by treating a solid support having hydrophilic moieties on its surface with a derivatizing composition containing a mixture of silanes. A first silane provides the desired reduction in surface energy, while the second silane enables functionalization with molecular moieties of interest, such as small molecules, initial monomers to be used in the solid phase synthesis of oligomers, or intact oligomers. Molecular moieties of interest may be attached through cleavable sites. Derivatizing compositions for carrying out the surface functionalization process are provided as well.
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6.发明授权公开(公告)号：US09273086B2
公开(公告)日：2016-03-01
申请号：US13531191
申请日：2012-06-22
申请人： Douglas J. Dellinger , Joel Myerson , Agnieszka B. Sierzchala , Geraldine F. Dellinger , Zoltan Timar
发明人： Douglas J. Dellinger , Joel Myerson , Agnieszka B. Sierzchala , Geraldine F. Dellinger , Zoltan Timar
IPC分类号： C07H21/02 , C07H13/12 , C07H19/067 , A61K31/7125 , A61K31/712 , C07H19/00 , C07H19/167 , C07H21/00 , C07H23/00
CPC分类号： C07H19/067 , A61K31/712 , A61K31/7125 , C07H1/00 , C07H13/12 , C07H19/00 , C07H19/167 , C07H21/00 , C07H21/02 , C07H21/04 , C07H23/00 , Y02P20/55
摘要： A method of deprotecting a solid support bound polynucleotide includes the step of contacting the polynucleotide with a composition comprising a diamine under conditions sufficient to deprotect the 2′-protected ribonucleotide residue. The solid support bound polynucleotide has at least one 2′-protected ribonucleotide residue, which has the following structure: wherein BP is a protected or unprotected heterocycle; R12 is a protecting group selected from a hydrocarbyl, a substituted hydrocarbyl, an aryl, and a substituted aryl; X is O or S; and PG is a thionocarbamate protecting group.
摘要翻译： 使固体支持物结合的多核苷酸去保护的方法包括在足以使2'-保护的核糖核苷酸残基脱保护的条件下使多核苷酸与包含二胺的组合物接触的步骤。 固体支持结合的多核苷酸具有至少一个2'-保护的核糖核苷酸残基，其具有以下结构：其中BP是被保护或未被保护的杂环; R 12是选自烃基，取代的烃基，芳基和取代的芳基的保护基; X是O或S; PG是硫代氨基甲酸酯保护基。
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公开(公告)号：US20120238737A1
公开(公告)日：2012-09-20
申请号：US13485592
申请日：2012-05-31
申请人： Douglas J. Dellinger , Agnieszka Sierzchala , John Turner , Joel Myerson , Zoltan Kupihar , Fernando Ferreira , Marvin H. Caruthers , Geraldine F. Dellinger
发明人： Douglas J. Dellinger , Agnieszka Sierzchala , John Turner , Joel Myerson , Zoltan Kupihar , Fernando Ferreira , Marvin H. Caruthers , Geraldine F. Dellinger
CPC分类号： C07H19/073 , C07H13/12 , C07H19/16 , C07H21/02 , Y02P20/55
摘要： Aspects of the invention include 2′ protected nucleoside monomers that are protected at the 2′ site with thiocarbon protecting groups. Thiocarbon protecting groups of interest include thiocarbonate, thionocarbonate, dithiocarbonate groups, as well as thionocarbamate protecting groups. Aspects of the invention further include nucleic acids that include the protecting groups of the invention, as well as methods of synthesizing nucleic acids using the protecting groups of the invention.
摘要翻译： 本发明的方面包括2'受保护的核苷单体，其在2'位置被硫代保护基团保护。 感兴趣的硫代碳保护基包括硫代碳酸酯，硫代碳酸酯，二硫代碳酸酯基，以及硫羰氨基保护基。 本发明的方面还包括包含本发明的保护基团的核酸以及使用本发明的保护基合成核酸的方法。
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公开(公告)号：US20090082554A1
公开(公告)日：2009-03-26
申请号：US11903821
申请日：2007-09-24
摘要： In various embodiments of the invention, novel compositions having a polynucleotide bound to a substrate via a cleavable linker are provided, and methods of cleaving a polynucleotide from a substrate are provided.
摘要翻译： 在本发明的各种实施方案中，提供了具有通过可切割连接子与底物结合的多核苷酸的新型组合物，并提供了从底物切割多核苷酸的方法。
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公开(公告)号：US20070224602A1
公开(公告)日：2007-09-27
申请号：US11389326
申请日：2006-03-23
CPC分类号： C07H21/04
摘要： In various embodiments of the invention, novel compositions having a polynucleotide bound to a substrate via a cleavable linker are provided, and methods of cleaving a polynucleotide from a substrate are provided.
摘要翻译： 在本发明的各种实施方案中，提供了具有通过可切割连接子与底物结合的多核苷酸的新型组合物，并提供了从底物切割多核苷酸的方法。
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公开(公告)号：US09067961B2
公开(公告)日：2015-06-30
申请号：US13485592
申请日：2012-05-31
申请人： Douglas J. Dellinger , Agnieszka B. Sierzchala , John Turner , Joel Myerson , Zoltan Kupihar , Fernando Ferreira , Marvin H. Caruthers , Geraldine F. Dellinger
发明人： Douglas J. Dellinger , Agnieszka B. Sierzchala , John Turner , Joel Myerson , Zoltan Kupihar , Fernando Ferreira , Marvin H. Caruthers , Geraldine F. Dellinger
IPC分类号： C07H13/12 , C07H21/02 , C07H19/073 , C07H19/16
CPC分类号： C07H19/073 , C07H13/12 , C07H19/16 , C07H21/02 , Y02P20/55
摘要： Aspects of the invention include 2′ protected nucleoside monomers that are protected at the 2′ site with thiocarbon protecting groups. Thiocarbon protecting groups of interest include thiocarbonate, thionocarbonate, dithiocarbonate groups, as well as thionocarbamate protecting groups. Aspects of the invention further include nucleic acids that include the protecting groups of the invention, as well as methods of synthesizing nucleic acids using the protecting groups of the invention.
摘要翻译： 本发明的方面包括2'受保护的核苷单体，其在2'位置被硫代保护基团保护。 感兴趣的硫代碳保护基包括硫代碳酸酯，硫代碳酸酯，二硫代碳酸酯基，以及硫羰氨基保护基。 本发明的方面还包括包含本发明的保护基团的核酸以及使用本发明的保护基合成核酸的方法。
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