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    Growth/differentiation factors of the TGF-.beta. family
    2.
    发明授权
    Growth/differentiation factors of the TGF-.beta. family 失效
    TGF-β家族的生长/分化因子

    公开(公告)号：US6120760A

    公开(公告)日：2000-09-19

    申请号：US289222

    申请日：1994-08-12

    申请人： Gertrud Hotten ,  Helge Neidhardt ,  Rolf Bechtold ,  Jens Pohl

    发明人： Gertrud Hotten ,  Helge Neidhardt ,  Rolf Bechtold ,  Jens Pohl

    IPC分类号： A61K38/00 ,  C07K14/495 ,  C07K14/51 ,  C12N15/19 ,  A61K38/19

    CPC分类号： C07K14/51 ,  C07K14/495 ,  A61K38/00

    摘要： The invention provides DNA sequences encoding novel members of the TGF-.beta. family of proteins. The TGF-.beta. family comprises proteins which function as growth and/or differentiation factors and which are useful in medical applications. Accordingly, the invention also describes the isolation of the above-mentioned DNA sequences, the expression of the encoded proteins, the production of the proteins and pharmaceutical compositions containing the proteins.

    摘要翻译： 本发明提供了编码TGF-β蛋白家族的新成员的DNA序列。 TGF-β家族包含起到生长和/或分化因子的作用，可用于医学应用的蛋白质。 因此，本发明还描述了分离上述DNA序列，编码蛋白质的表达，蛋白质的产生和含有蛋白质的药物组合物。

    Process for preparing purified active monomer of bone-derived factor
    6.
    发明申请
    Process for preparing purified active monomer of bone-derived factor 失效
    骨源性因子纯化活性单体的制备方法

    公开(公告)号：US20050026247A1

    公开(公告)日：2005-02-03

    申请号：US10734583

    申请日：2003-12-15

    申请人： Hidetoshi Andou ,  Jun Honda ,  Shunjiro Sugimoto ,  Gertrud Hotten ,  Rolf Bechtold ,  Jens Pohl

    发明人： Hidetoshi Andou ,  Jun Honda ,  Shunjiro Sugimoto ,  Gertrud Hotten ,  Rolf Bechtold ,  Jens Pohl

    IPC分类号： A61K38/00 ,  C07K14/475 ,  C07K14/51 ,  C07K14/575 ,  C12N1/21 ,  C07H21/04

    CPC分类号： C07K14/51 ,  A61K38/00 ,  C07K14/475 ,  C07K14/575

    摘要： A process for preparing a purified refolded monomer or dimer of a bone-derived factor, which comprises subjecting an inclusion body of a bone-derived factor produced by genetic engineering to the following steps a) to c) in sequence: a) introducing a polynucleotide encoding a bone morphogenetic factor into a bacterium, expressing said bone morphogenetic factor in the form of an inclusion body, recovering said inclusion body and treating it with a denaturing agent to obtain a solubilized monomer, b) treating the solubilized monomer without purification directly with a refolding solution to obtain a refolded monomeric bone morphogenetic factor, c) subjecting the refolded monomeric bone morphogenetic factor to purification.

    摘要翻译： 一种制备骨源性因子的纯化的重折叠单体或二聚体的方法，其包括使基因工程产生的骨衍生因子的内含体按顺序步骤a）至c）：a）将多核苷酸 将骨形态发生因子编码成细菌，以包涵体的形式表达所述骨形态发生因子，回收所述包涵体并用变性剂处理以获得溶解的单体，b）直接用 重折叠溶液以获得重折叠的单体骨形态发生因子，c）使重折叠的单体骨形态发生因子进行纯化。