公开(公告)号：US20150154352A1

公开(公告)日：2015-06-04

申请号：US14409452

申请日：2013-06-21

Applicant: GigaGen, Inc.

Inventor： David Scott Johnson ,  Andrea Loehr ,  Thomas Hunt ,  Everett Hurteau Meyer ,  Walter Mathias Howell ,  Gary Withey

IPC: G06F19/22 ,  G01N33/68 ,  G01N33/50 ,  C12Q1/68 ,  G01N33/487

CPC classification number: G16B30/00 ,  C12Q1/6809 ,  C12Q1/686 ,  C12Q1/6881 ,  C12Q2600/16 ,  G01N33/487 ,  G01N33/5005 ,  G01N33/68 ,  C12Q2535/122 ,  C12Q2537/165

Abstract: Methods and systems are provided for massively parallel genetic analysis of single cells in emulsions or droplets. A biological sample is divided into subsamples of single cells or cell supbopulations, and a fusion complex is formed by molecular linkage and amplification techniques. Methods, apparatuses, and systems are provided for high-throughput, massively parallel analysis of the subsamples. These methods integrate molecular, algorithmic, and engineering approaches. They have broad and useful application in a number of biological and medical fields, including immunology, noninvasive prenatal diagnosis, and noninvasive cancer diagnosis.

Abstract translation: 提供了方法和系统用于在乳液或液滴中对单个细胞的大规模并行遗传分析。 将生物样品分为单细胞或细胞支原体的子样本，通过分子连锁和扩增技术形成融合复合体。 提供了方法，装置和系统用于子采样的高通量，大规模并行分析。 这些方法集成了分子，算法和工程方法。 它们在许多生物和医学领域具有广泛和有用的应用，包括免疫学，无创产前诊断和非侵入性癌症诊断。