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公开(公告)号：US20150125865A1
公开(公告)日：2015-05-07
申请号：US14368091
申请日：2012-12-20
Applicant: GigaGen, Inc.
Inventor： David Scott Johnson , Gary Withey , Philip Homewood , Timothy Atkins
CPC classification number: C12N1/06 , B01F3/08 , B01F13/0071 , B01F13/0094 , B01F2215/0422 , B01F2215/0431 , B01L3/502715 , B01L3/50273 , B01L3/502784 , B01L2200/0647 , B01L2300/0867 , B01L2300/18 , B81B7/00 , G01N35/08
Abstract: Methods and systems are provided for merging a droplet with a volume of fluid in a microfluidic system. In particular, the methods of the invention use a microfluidic structure designed to merge a fluid with a droplet in order to dilute, add volume, or add selected reagents, biological materials, or synthetic materials to a droplet. Also provided are related systems and methods for cell lysis.
Abstract translation: 提供了用于在微流体系统中将液滴与一定体积的流体合并的方法和系统。 特别地，本发明的方法使用设计用于将流体与液滴合并的微流体结构，以稀释，添加体积或将选定的试剂，生物材料或合成材料添加到液滴中。 还提供了细胞裂解的相关系统和方法。
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公开(公告)号：US20150154352A1
公开(公告)日：2015-06-04
申请号：US14409452
申请日：2013-06-21
Applicant: GigaGen, Inc.
Inventor： David Scott Johnson , Andrea Loehr , Thomas Hunt , Everett Hurteau Meyer , Walter Mathias Howell , Gary Withey
IPC: G06F19/22 , G01N33/68 , G01N33/50 , C12Q1/68 , G01N33/487
CPC classification number: G16B30/00 , C12Q1/6809 , C12Q1/686 , C12Q1/6881 , C12Q2600/16 , G01N33/487 , G01N33/5005 , G01N33/68 , C12Q2535/122 , C12Q2537/165
Abstract: Methods and systems are provided for massively parallel genetic analysis of single cells in emulsions or droplets. A biological sample is divided into subsamples of single cells or cell supbopulations, and a fusion complex is formed by molecular linkage and amplification techniques. Methods, apparatuses, and systems are provided for high-throughput, massively parallel analysis of the subsamples. These methods integrate molecular, algorithmic, and engineering approaches. They have broad and useful application in a number of biological and medical fields, including immunology, noninvasive prenatal diagnosis, and noninvasive cancer diagnosis.
Abstract translation: 提供了方法和系统用于在乳液或液滴中对单个细胞的大规模并行遗传分析。 将生物样品分为单细胞或细胞支原体的子样本，通过分子连锁和扩增技术形成融合复合体。 提供了方法，装置和系统用于子采样的高通量，大规模并行分析。 这些方法集成了分子，算法和工程方法。 它们在许多生物和医学领域具有广泛和有用的应用，包括免疫学，无创产前诊断和非侵入性癌症诊断。
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公开(公告)号：US20160264924A1
公开(公告)日：2016-09-15
申请号：US15160671
申请日：2016-05-20
Applicant: GigaGen, Inc.
Inventor： David Scott Johnson , Gary Withey , Philip Homewood , Timothy Atkins
CPC classification number: C12N1/06 , B01F3/08 , B01F13/0071 , B01F13/0094 , B01F2215/0422 , B01F2215/0431 , B01L3/502715 , B01L3/50273 , B01L3/502784 , B01L2200/0647 , B01L2300/0867 , B01L2300/18 , B81B7/00 , G01N35/08
Abstract: Methods and systems are provided for merging a droplet with a volume of fluid in a microfluidic system. In particular, the methods of the invention use a microfluidic structure designed to merge a fluid with a droplet in order to dilute, add volume, or add selected reagents, biological materials, or synthetic materials to a droplet. Also provided are related systems and methods for cell lysis.
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