公开(公告)号：US10119975B2

公开(公告)日：2018-11-06

申请号：US15363243

申请日：2016-11-29

申请人： Ginkgo Bioworks, Inc.

发明人： Reshma Shetty ,  Thomas F. Knight, Jr. ,  Randall D. Rettberg

IPC分类号： C12N15/70 ,  G01N33/68 ,  C12N15/74 ,  C40B40/08

摘要： Systems, methods, libraries, kits, and computer software tools are provided for designing and producing engineered cells. Such engineered cells can be used for cell state quantification, such as genome, transcriptome and/or proteome quantification. In one aspect, an engineered cell having a plurality of artificially designed oligonucleotides introduced into the genome of the cell is provided. The oligonucleotides are each located in proximity of a gene of interest encoding a protein of interest, and are different from one another. The oligonucleotides can each encode a unique peptide tag for each protein of interest, wherein each peptide tag has a unique quantitatively measurable value such as mass-to-charge ratio which can be quantified by a mass spectrometer. The engineered cell is capable of expressing a plurality of proteins of interest each fused to its corresponding unique peptide tag, wherein each peptide tag is capable of being released therefrom.

公开(公告)号：US20170074889A1

公开(公告)日：2017-03-16

申请号：US15363243

申请日：2016-11-29

申请人： Ginkgo Bioworks, Inc.

发明人： Reshma Shetty ,  Thomas F. Knight, JR. ,  Randall D. Rettberg

IPC分类号： G01N33/68 ,  C12N15/70 ,  C12N15/74

CPC分类号： G01N33/6848 ,  C12N15/70 ,  C12N15/74 ,  C40B40/08

摘要： Systems, methods, libraries, kits, and computer software tools are provided for designing and producing engineered cells. Such engineered cells can be used for cell state quantification, such as genome, transcriptome and/or proteome quantification. In one aspect, an engineered cell having a plurality of artificially designed oligonucleotides introduced into the genome of the cell is provided. The oligonucleotides are each located in proximity of a gene of interest encoding a protein of interest, and are different from one another. The oligonucleotides can each encode a unique peptide tag for each protein of interest, wherein each peptide tag has a unique quantitatively measurable value such as mass-to-charge ratio which can be quantified by a mass spectrometer. The engineered cell is capable of expressing a plurality of proteins of interest each fused to its corresponding unique peptide tag, wherein each peptide tag is capable of being released therefrom.

摘要翻译： 提供系统，方法，库，套件和计算机软件工具，用于设计和生产工程细胞。 这样的工程细胞可用于细胞状态定量，例如基因组，转录组和/或蛋白质组学定量。 在一个方面，提供了具有引入细胞基因组的多个人造设计寡核苷酸的工程细胞。 寡核苷酸各自位于感兴趣的蛋白质的编码基因附近，并且彼此不同。 寡核苷酸可以各自编码每个感兴趣的蛋白质的独特的肽标签，其中每个肽标签具有唯一的可定量测量的值，例如可以通过质谱仪量化的质荷比。 工程细胞能够表达多个与其相应的唯一肽标签融合的感兴趣的蛋白质，其中每个肽标签能够从其释放。