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    Simultaneous human ABO and RH(D) blood typing or antibody screening by flow cytometry
    1.
    发明授权
    Simultaneous human ABO and RH(D) blood typing or antibody screening by flow cytometry 失效
    通过流式细胞术同时进行人ABO和RH（D）血型或抗体筛选

    公开(公告)号：US5776711A

    公开(公告)日：1998-07-07

    申请号：US747558

    申请日：1996-11-12

    申请人： Girish N. Vyas ,  Kodumudi Venkateswaran

    发明人： Girish N. Vyas ,  Kodumudi Venkateswaran

    IPC分类号： G01N33/80 ,  G01N33/53 ,  G01N33/555

    CPC分类号： G01N33/80

    摘要： Flow cytometric methodology is provided for simultaneous determination of (1) ABO and Rh(D) typing of human red cells, (2) natural isoantibodies in plasma, and (3) screening for alloantibodies in plasma. The method includes (a) the use of a unique combination of fluorescent labelled antibodies to A, B and Rh(D) antigens to carry out (1); (b) different sized beads coated with blood group substances A & B to carry out (2); and (c) the differential fluorescent labelling of screening reagent red blood cells for flow cytometric analyses to carry out (3). The routine ABO and Rh(D) typing and antibody screening of human blood for both isoantibodies and alloantibodies can be determined in three individual reactions compared to 7 to 10 tests currently performed in blood banks.

    摘要翻译： 提供流式细胞术方法用于同时测定（1）人红细胞的ABO和Rh（D）分型，（2）血浆中的天然异抗体，和（3）筛选血浆中的同种抗体。 该方法包括（a）使用荧光标记抗体对A，B和Rh（D）抗原的独特组合进行（1）; （b）涂有血型物质A和B的不同大小的珠子进行（2）; 和（c）筛选试剂红细胞的差异荧光标记用于流式细胞术分析进行（3）。 可以在三个单独的反应中测定人类血液中同种抗体和同种异体抗体的常规ABO和Rh（D）分型和抗体筛选，与目前在血库中进行的7至10次测试相比。

    Flow cytometric detection method for DNA samples
    2.
    发明申请
    Flow cytometric detection method for DNA samples 有权
    DNA样品的流式细胞检测方法

    公开(公告)号：US20070117110A1

    公开(公告)日：2007-05-24

    申请号：US11454478

    申请日：2006-06-16

    申请人： Shanavaz Nasarabadi ,  Richard Langlois ,  Kodumudi Venkateswaran

    发明人： Shanavaz Nasarabadi ,  Richard Langlois ,  Kodumudi Venkateswaran

    IPC分类号： C12Q1/68 ,  C12P19/34 ,  C07H21/04

    CPC分类号： C12Q1/6851 ,  C12Q1/686 ,  C12Q2565/626 ,  C12Q2563/155 ,  C12Q2561/101 ,  C12Q2537/143

    摘要： Disclosed herein are two methods for rapid multiplex analysis to determine the presence and identity of target DNA sequences within a DNA sample. Both methods use reporting DNA sequences, e.g., modified conventional Taqman® probes, to combine multiplex PCR amplification with microsphere-based hybridization using flow cytometry means of detection. Real-time PCR detection can also be incorporated. The first method uses a cyanine dye, such as, Cy3™, as the reporter linked to the 5′ end of a reporting DNA sequence. The second method positions a reporter dye, e.g., FAM, on the 3′ end of the reporting DNA sequence and a quencher dye, e.g., TAMRA, on the 5′ end.

    摘要翻译： 本文公开了用于快速多重分析以确定DNA样品中靶DNA序列的存在和身份的两种方法。 两种方法使用报告DNA序列，例如修饰的常规Taqman探针，以使用流式细胞术检测方法将多重PCR扩增与基于微球的杂交组合。 也可以并入实时PCR检测。 第一种方法使用花青染料，例如Cy3 TM作为连接到报告DNA序列的5'末端的报告物。 第二种方法将报告染料（例如FAM）置于报告DNA序列的3'末端和5'末端的猝灭剂染料（例如TAMRA）上。