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    Method for the efficiency-corrected real-time quantification of nucleic acids
    1.
    发明授权
    Method for the efficiency-corrected real-time quantification of nucleic acids 有权
    核酸的效率校正实时定量的方法

    公开(公告)号：US06691041B2

    公开(公告)日：2004-02-10

    申请号：US09823711

    申请日：2001-03-30

    申请人： Gregor Sagner ,  Karim Tabiti ,  Martin Gutekunst ,  Richie Soong

    发明人： Gregor Sagner ,  Karim Tabiti ,  Martin Gutekunst ,  Richie Soong

    IPC分类号： G01N3348

    CPC分类号： G06F19/00 ,  C12Q1/6851 ,  C12Q2561/113 ,  C12Q2545/114 ,  C12Q2545/113

    摘要： The present invention concerns a method for the quantification of a target nucleic acid in a sample comprising the following steps: (i) determination of the amplification efficiency of the target nucleic acid under defined amplification conditions, (ii) amplification of the target nucleic acid contained in the sample under the same defined reaction conditions, (iii) measuring the amplification in real-time, (iv) quantification of the original amount of target nucleic acid in the sample by correction of the original amount derived from step (iii) with the aid of the determined amplification efficiency. The efficiency correction of PCR reactions according to the invention for the quantification of nucleic acids can be used for absolute quantification with the aid of an external or internal standard as well as for relative quantification compared to the expression of housekeeping genes.

    摘要翻译： 本发明涉及用于定量样品中靶核酸的方法，包括以下步骤：（i）在确定的扩增条件下测定靶核酸的扩增效率，（ii）扩增含有的靶核酸 在相同定义的反应条件下的样品中，（iii）实时测量扩增，（iv）通过用步骤（iii）得到的原始量的校正来定量样品中目标核酸的原始量， 有助于确定的扩增效率。 根据本发明的用于定量核酸的PCR反应的效率校正可以用于借助于外部或内部标准的绝对定量，以及与管家基因的表达相比进行相对定量。

    Method for the efficiency-corrected real-time quantification of nucleic acids
    2.
    发明授权
    Method for the efficiency-corrected real-time quantification of nucleic acids 有权
    核酸的效率校正实时定量的方法

    公开(公告)号：US08024132B2

    公开(公告)日：2011-09-20

    申请号：US12549150

    申请日：2009-08-27

    申请人： Gregor Sagner ,  Karim Tabiti ,  Martin Gutekunst ,  Richie Soong

    发明人： Gregor Sagner ,  Karim Tabiti ,  Martin Gutekunst ,  Richie Soong

    IPC分类号： G01N33/48 ,  G01N1/00

    CPC分类号： G06F19/00 ,  C12Q1/6851 ,  C12Q2561/113 ,  C12Q2545/114 ,  C12Q2545/113

    摘要： The present invention concerns a method for the quantification of a target nucleic acid in a sample comprising the following steps: (i) determination of the amplification efficiency of the target nucleic acid under defined amplification conditions, (ii) amplification of the target nucleic acid contained in the sample under the same defined reaction conditions, (iii) measuring the amplification in real-time, (iv) quantification of the original amount of target nucleic acid in the sample by correction of the original amount derived from step (iii) with the aid of the determined amplification efficiency. The efficiency correction of PCR reactions according to the invention for the quantification of nucleic acids can be used for absolute quantification with the aid of an external or internal standard as well as for relative quantification compared to the expression of housekeeping genes.

    摘要翻译： 本发明涉及用于定量样品中靶核酸的方法，包括以下步骤：（i）在确定的扩增条件下测定靶核酸的扩增效率，（ii）扩增含有的靶核酸 在相同定义的反应条件下的样品中，（iii）实时测量扩增，（iv）通过用步骤（iii）得到的原始量的校正来定量样品中目标核酸的原始量， 有助于确定的扩增效率。 根据本发明的用于定量核酸的PCR反应的效率校正可以用于借助于外部或内部标准的绝对定量，以及与管家基因的表达相比进行相对定量。

    METHOD FOR THE EFFICIENCY-CORRECTED REAL-TIME QUANTIFICATION OF NUCLEIC ACIDS
    3.
    发明申请
    METHOD FOR THE EFFICIENCY-CORRECTED REAL-TIME QUANTIFICATION OF NUCLEIC ACIDS 有权
    用于有效校正核酸实时定量的方法

    公开(公告)号：US20100021923A1

    公开(公告)日：2010-01-28

    申请号：US12549150

    申请日：2009-08-27

    申请人： Gregor Sagner ,  Karim Tabiti ,  Martin Gutekunst ,  Richie Soong

    发明人： Gregor Sagner ,  Karim Tabiti ,  Martin Gutekunst ,  Richie Soong

    IPC分类号： C12Q1/68

    CPC分类号： G06F19/00 ,  C12Q1/6851 ,  C12Q2561/113 ,  C12Q2545/114 ,  C12Q2545/113

    摘要： The present invention concerns a method for the quantification of a target nucleic acid in a sample comprising the following steps: (i) determination of the amplification efficiency of the target nucleic acid under defined amplification conditions, (ii) amplification of the target nucleic acid contained in the sample under the same defined reaction conditions, (iii) measuring the amplification in real-time, (iv) quantification of the original amount of target nucleic acid in the sample by correction of the original amount derived from step (iii) with the aid of the determined amplification efficiency. The efficiency correction of PCR reactions according to the invention for the quantification of nucleic acids can be used for absolute quantification with the aid of an external or internal standard as well as for relative quantification compared to the expression of housekeeping genes.

    摘要翻译： 本发明涉及用于定量样品中靶核酸的方法，包括以下步骤：（i）在确定的扩增条件下测定靶核酸的扩增效率，（ii）扩增含有的靶核酸 在相同定义的反应条件下的样品中，（iii）实时测量扩增，（iv）通过用步骤（iii）得到的原始量的校正来定量样品中目标核酸的原始量， 有助于确定的扩增效率。 根据本发明的用于定量核酸的PCR反应的效率校正可以用于借助于外部或内部标准的绝对定量，以及与管家基因的表达相比进行相对定量。

    Method for real-time quantification of nucleic acids
    4.
    发明授权
    Method for real-time quantification of nucleic acids 有权
    核酸实时定量的方法

    公开(公告)号：US08744777B2

    公开(公告)日：2014-06-03

    申请号：US13163500

    申请日：2011-06-17

    申请人： Gregor Sagner ,  Karim Tabiti ,  Martin Gutekunst ,  Richie Soong

    发明人： Gregor Sagner ,  Karim Tabiti ,  Martin Gutekunst ,  Richie Soong

    IPC分类号： G01N33/48 ,  G06F19/00

    CPC分类号： G06F19/00 ,  C12Q1/6851 ,  C12Q2561/113 ,  C12Q2545/114 ,  C12Q2545/113

    摘要： The present invention concerns a method for the quantification of a target nucleic acid in a sample comprising the following steps: (i) determination of the amplification efficiency of the target nucleic acid under defined amplification conditions, (ii) amplification of the target nucleic acid contained in the sample under the same defined reaction conditions, (iii) measuring the amplification in real-time, (iv) quantification of the original amount of target nucleic acid in the sample by correction of the original amount derived from step (iii) with the aid of the determined amplification efficiency. The efficiency correction of PCR reactions according to the invention for the quantification of nucleic acids can be used for absolute quantification with the aid of an external or internal standard as well as for relative quantification compared to the expression of housekeeping genes.

    摘要翻译： 本发明涉及用于定量样品中靶核酸的方法，包括以下步骤：（i）在确定的扩增条件下测定靶核酸的扩增效率，（ii）扩增含有的靶核酸 在相同定义的反应条件下的样品中，（iii）实时测量扩增，（iv）通过用步骤（iii）得到的原始量的校正来定量样品中目标核酸的原始量， 有助于确定的扩增效率。 根据本发明的用于定量核酸的PCR反应的效率校正可以用于借助于外部或内部标准的绝对定量，以及与管家基因的表达相比进行相对定量。

    Method for determining the efficiency of nucleic acid amplifications
    5.
    发明授权
    Method for determining the efficiency of nucleic acid amplifications 有权
    确定核酸扩增效率的方法

    公开(公告)号：US07378241B2

    公开(公告)日：2008-05-27

    申请号：US10810101

    申请日：2004-03-25

    申请人： Gregor Sagner ,  Karim Tabiti ,  Martin Gutekunst ,  Richie Soong

    发明人： Gregor Sagner ,  Karim Tabiti ,  Martin Gutekunst ,  Richie Soong

    IPC分类号： C12Q1/68 ,  C12P19/34 ,  C07H21/04

    CPC分类号： C12Q1/686 ,  C12Q1/6851 ,  C12Q2561/113 ,  C12Q2527/143

    摘要： The present invention concerns a method for determining the efficiency of the amplification of a target nucleic acid comprising the following steps: (i) preparation of a dilution series of the target nucleic acid, (ii) amplifying the target nucleic acid under defined reaction conditions, the amplification being measured in real-time (iii) setting a defined signal threshold value, (iv) determining the cycle number at which the signal threshold value is exceeded for various dilutions, (v) determining the amplification efficiency as a function of the amount of original target nucleic acid. The present invention also concerns a method for the quantification of a target nucleic acid in a sample in which the efficiency of the amplification reaction is determined in this manner and is taken into account in the quantification.

    摘要翻译： 本发明涉及用于确定靶核酸扩增效率的方法，包括以下步骤：（i）制备目标核酸的稀释系列，（ii）在规定的反应条件下扩增靶核酸， 实时测量放大（iii）设定定义的信号阈值，（iv）确定各种稀释度超过信号阈值的周期数，（v）确定作为量的函数的放大效率 的原始靶核酸。 本发明还涉及用于定量样品中靶核酸的方法，其中以这种方式确定扩增反应的效率并在定量中考虑。

    Method for determining the efficiency of nucleic acid amplifications
    7.
    发明申请
    Method for determining the efficiency of nucleic acid amplifications 有权
    确定核酸扩增效率的方法

    公开(公告)号：US20050009048A1

    公开(公告)日：2005-01-13

    申请号：US10810101

    申请日：2004-03-25

    申请人： Gregor Sagner ,  Karim Tabiti ,  Martin Gutekunst ,  Richie Soong

    发明人： Gregor Sagner ,  Karim Tabiti ,  Martin Gutekunst ,  Richie Soong

    IPC分类号： G01N33/50 ,  C12N15/09 ,  C12Q1/68 ,  G01N33/48 ,  G06F19/00

    CPC分类号： C12Q1/686 ,  C12Q1/6851 ,  C12Q2561/113 ,  C12Q2527/143

    摘要： The present invention concerns a method for determining the efficiency of the amplification of a target nucleic acid comprising the following steps: (i) preparation of a dilution series of the target nucleic acid, (ii) amplifying the target nucleic acid under defined reaction conditions, the amplification being measured in real-time (iii) setting a defined signal threshold value, (iv) determining the cycle number at which the signal threshold value is exceeded for various dilutions, (v) determining the amplification efficiency as a function of the amount of original target nucleic acid. The present invention also concerns a method for the quantification of a target nucleic acid in a sample in which the efficiency of the amplification reaction is determined in this manner and is taken into account in the quantification.

    摘要翻译： 本发明涉及用于确定靶核酸扩增效率的方法，包括以下步骤：（i）制备目标核酸的稀释系列，（ii）在规定的反应条件下扩增靶核酸， 实时测量放大（iii）设定定义的信号阈值，（iv）确定各种稀释度超过信号阈值的周期数，（v）确定作为量的函数的放大效率 的原始靶核酸。 本发明还涉及用于定量样品中靶核酸的方法，其中以这种方式确定扩增反应的效率并在定量中考虑。