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13 条记录 (耗时 0.031 秒)

    Dynamic spectral phonocardiograph
    5.
    发明授权
    Dynamic spectral phonocardiograph 失效
    动态光谱心电图仪

    公开(公告)号：US5012815A

    公开(公告)日：1991-05-07

    申请号：US533544

    申请日：1990-06-05

    申请人： William R. Bennett, Jr. ,  Jean Bennett

    发明人： William R. Bennett, Jr. ,  Jean Bennett

    IPC分类号： A61B7/04

    CPC分类号： A61B7/04

    摘要： A system of generating a spectral phonocardiogram which summarizes time-dependent changes in the heart sounds throughout the heart cycle. The system is based on the projection of spectral surfaces of the Fourier transform of heart sounds as a function of time and is a useful diagnostic tool both for a cardiologist and a general practitioner. Permanent copies of the spectral phonocardiograms can provide useful records for monitoring the development of heart disease in a given individual. The system provides a useful means for rapid screening of large groups of people for heart disease by non-specialists in cardiology. A variant of the system provides automated computer diagnosis of the probable nature of the heart disease.

    摘要翻译： 产生频谱心电图的系统，其总结心脏周期中心音的时间依赖性变化。 该系统基于心音的傅立叶变换的时间的函数的投影，并且是心脏病学家和全科医生的有用的诊断工具。 光谱心电图的永久性拷贝可以为监测给定个体心脏病发展提供有用的记录。 该系统为心脏病专家的非专家快速筛查大量心脏病患者提供了有用的手段。 该系统的变体提供对心脏病的可能性质的自动计算机诊断。

    Proviral plasmids and production of recombinant adeno-associated virus
    6.
    发明授权
    Proviral plasmids and production of recombinant adeno-associated virus 有权
    丙型肝炎病毒质粒和重组腺相关病毒的产生

    公开(公告)号：US09249425B2

    公开(公告)日：2016-02-02

    申请号：US14117312

    申请日：2012-05-16

    申请人： Jean Bennett ,  Jeannette L. Bennicelli

    发明人： Jean Bennett ,  Jeannette L. Bennicelli

    IPC分类号： C07K14/435 ,  C12N15/86 ,  C12N15/35 ,  C12N15/864

    CPC分类号： C12N7/00 ,  C07K14/435 ,  C12N15/86 ,  C12N2750/14111 ,  C12N2750/14121 ,  C12N2750/14141 ,  C12N2750/14143 ,  C12N2750/14152 ,  C12N2800/107 ,  C12N2830/60 ,  C12N2830/90

    摘要： Proviral plasmids contain a modular gene expression cassette with one or a combination of (i) a wildtype 5′ AAV2 ITR sequence flanked by unique restriction sites that permit ready removal or replacement of said ITR; (ii) a promoter flanked by unique restriction sites that permit ready removal or replacement of the entire promoter sequence; (iii) a polylinker sequence that permits insertion of a gene coding sequence without modification thereof, wherein the gene is operatively linked to, and under the regulatory control of, the aforementioned promoter; (iv) a bovine growth hormone polyadenylation sequence flanked by unique restriction sites that permit ready removal or replacement of said polyA sequence; and (v) a wildtype 3′ AAV2 ITR sequence flanked by unique restriction sites that permit ready removal or replacement of the 3′ ITR. These plasmids enable rapid manipulation of the components of the cassette, e.g., rapid mutation and/or replacement of any component, and thereby increase the efficiency of recombinant viral vector, e.g., rAAV, production.

    摘要翻译： 丙型肝炎病毒质粒包含一个或多个组合的模块化基因表达盒，（i）野生型5'AAV2 ITR序列，其侧翼是唯一的限制性位点，允许准备移除或替换所述ITR; （ii）侧翼为独特的限制性位点的启动子，其允许准备去除或置换整个启动子序列; （iii）允许插入基因编码序列而无需修饰的多接头序列，其中所述基因与上述启动子有效连接并在其调控下控制; （iv）两侧有唯一限制性位点的牛生长激素多聚腺苷酸化序列，其允许准备移除或替换所述polyA序列; 和（v）野生型3'AAV2 ITR序列，其侧翼是唯一的限制性位点，允许准备移除或替换3'ITR。 这些质粒能够快速操作盒的组分，例如任何组分的快速突变和/或置换，从而提高重组病毒载体如rAAV产生的效率。

    Proviral Plasmids and Production of Recombinant Adeno-Associated Virus
    7.
    发明申请
    Proviral Plasmids and Production of Recombinant Adeno-Associated Virus 有权
    病毒质粒和重组腺相关病毒的生产

    公开(公告)号：US20140087444A1

    公开(公告)日：2014-03-27

    申请号：US14117312

    申请日：2012-05-16

    申请人： Jean Bennett ,  Jeannette L. Bennicelli

    发明人： Jean Bennett ,  Jeannette L. Bennicelli

    IPC分类号： C12N15/86

    CPC分类号： C12N7/00 ,  C07K14/435 ,  C12N15/86 ,  C12N2750/14111 ,  C12N2750/14121 ,  C12N2750/14141 ,  C12N2750/14143 ,  C12N2750/14152 ,  C12N2800/107 ,  C12N2830/60 ,  C12N2830/90

    摘要： Proviral plasmids contain a modular gene expression cassette with one or a combination of (i) a wildtype 5′ AAV2 ITR sequence flanked by unique restriction sites that permit ready removal or replacement of said ITR; (ii) a promoter flanked by unique restriction sites that permit ready removal or replacement of the entire promoter sequence; (iii) a polylinker sequence that permits insertion of a gene coding sequence without modification thereof, wherein the gene is operatively linked to, and under the regulatory control of, the aforementioned promoter; (iv) a bovine growth hormone polyadenylation sequence flanked by unique restriction sites that permit ready removal or replacement of said polyA sequence; and (v) a wildtype 3′ AAV2 ITR sequence flanked by unique restriction sites that permit ready removal or replacement of the 3′ ITR. These plasmids enable rapid manipulation of the components of the cassette, e.g., rapid mutation and/or replacement of any component, and thereby increase the efficiency of recombinant viral vector, e.g., rAAV, production.

    摘要翻译： 丙型肝炎病毒质粒包含一个或多个组合的模块化基因表达盒，（i）野生型5'AAV2 ITR序列，其侧翼是唯一的限制性位点，允许准备移除或替换所述ITR; （ii）侧翼为独特的限制性位点的启动子，其允许准备去除或置换整个启动子序列; （iii）允许插入基因编码序列而无需修饰的多接头序列，其中所述基因与上述启动子有效连接并在其调控下控制; （iv）两侧有唯一限制性位点的牛生长激素多聚腺苷酸化序列，其允许准备移除或替换所述polyA序列; 和（v）野生型3'AAV2 ITR序列，其侧翼是唯一的限制性位点，允许准备移除或替换3'ITR。 这些质粒能够快速操作盒的组分，例如任何组分的快速突变和/或置换，从而提高重组病毒载体如rAAV产生的效率。

    AAV Vectors Expressing SEC10 for Treating Kidney Damage
    8.
    发明申请
    AAV Vectors Expressing SEC10 for Treating Kidney Damage 审中-公开
    表达SEC10用于治疗肾损伤的AAV载体

    公开(公告)号：US20120244127A1

    公开(公告)日：2012-09-27

    申请号：US13499212

    申请日：2010-09-30

    申请人： Joshua H. Lipschutz ,  Jean Bennett ,  Daniel C. Chung

    发明人： Joshua H. Lipschutz ,  Jean Bennett ,  Daniel C. Chung

    IPC分类号： A61K35/76 ,  C12N5/10 ,  A61P13/12 ,  C12N15/864

    CPC分类号： A61K48/0075 ,  A61K48/00 ,  C12N7/00 ,  C12N15/86 ,  C12N2710/10032 ,  C12N2750/14143

    摘要： A method for enhancing repair of damaged mammalian tubular epithelial cells involves delivering to the tubular epithelial cells of a subject in need thereof a composition comprising an adeno-associated virus (AAV) comprising an AAV capsid having an amino acid sequence of a selected AAV serotype, and a minigene having AAV inverted terminal repeats and a Sec10 gene operatively linked to regulatory sequences that direct expression of Sec10 in the epithelial cells. In one embodiment, delivery is accomplished by retrograde intrauretal injection. In an embodiment the AAV vector includes a capsid of AAV serotype 2/8. Therapeutic compositions containing such AAV are provided.

    摘要翻译： 增强损伤的哺乳动物肾小管上皮细胞修复的方法涉及向有需要的对象的管状上皮细胞递送包含腺伴随病毒（AAV）的组合物，其包含具有所选AAV血清型氨基酸序列的AAV衣壳， 和具有AAV反向末端重复的小基因和与指导Sec10在上皮细胞中表达的调节序列可操作地连接的Sec10基因。 在一个实施方案中，通过逆行心内注射实现递送。 在一个实施方案中，AAV载体包括AAV血清型2/8的衣壳。 提供含有这种AAV的治疗组合物。

    METHOD FOR TRANSDUCING CELLS WITH PRIMARY CILIA
    10.
    发明申请
    METHOD FOR TRANSDUCING CELLS WITH PRIMARY CILIA 审中-公开
    用初级CILIA传播细胞的方法

    公开(公告)号：US20100297084A1

    公开(公告)日：2010-11-25

    申请号：US12601898

    申请日：2008-05-30

    申请人： Jean Bennett ,  Joshua Lipschutz

    发明人： Jean Bennett ,  Joshua Lipschutz

    IPC分类号： A61K48/00 ,  C12N15/86 ,  A61P13/12 ,  A61P27/02

    摘要： This invention provides methods for transducing a ciliated cell with a recombinant serotype 2 adeno-associated virus (AAV) vector. Additionally, the invention provides methods of treating diseases associated with a mutated gene by transducing a ciliated cell with a recombinant serotype 2 AAV vector containing a corrective transgene.

    摘要翻译： 本发明提供了用重组血清型2腺相关病毒（AAV）载体转导纤毛细胞的方法。 另外，本发明提供了通过用含有修复转基因的重组血清型2 AAV载体转导纤毛细胞来治疗与突变基因相关的疾病的方法。