公开(公告)号：US5792649A

公开(公告)日：1998-08-11

申请号：US154283

申请日：1993-11-17

申请人： Guy Richard della-Cioppa ,  Stephen John Garger, Jr. ,  Richard Barry Holtz ,  Michael Jay McCulloch ,  Genadie Gleb Sverlow

发明人： Guy Richard della-Cioppa ,  Stephen John Garger, Jr. ,  Richard Barry Holtz ,  Michael Jay McCulloch ,  Genadie Gleb Sverlow

IPC分类号： C12N15/09 ,  C08G61/00 ,  C08H99/00 ,  C12N20060101 ,  C12N1/21 ,  C12N9/02 ,  C12N9/96 ,  C12N15/53 ,  C12P20060101 ,  C12P7/00 ,  C12P17/00 ,  C12P21/08 ,  C12R1/48 ,  C12N1/20

CPC分类号： C12Y110/03001 ,  C08G61/00 ,  C12N9/0059 ,  C12N9/0071 ,  C12P17/00 ,  C12Y114/18001

摘要： A process for the in vitro production of chemically modified polyphenolic polymer (PPP). First, stable, highly active extracellular tyrosinase is produced from genetically transformed microorganism such as Streptomyces antibioticus. The tyrosinase is then incubated with a reaction substrate such as l-tyrosine, hydrolyzed protein, or an oligopeptide in combination with l-tyrosine. The ratio of the oligopeptide/tyrosine combination as well as variation in the concentration of tyrosinase can be used to modify the color, the molecular size, and the spectral absorbance properties of the PPP produced. Alternatively, or additionally, oxidants such as hydrogen peroxide or hypochlorite can be used to modify the color of the PPP, regardless of the method used to produce the PPP, and the PPP can subsequently be fractionated using molecular weight cut-off ultrafiltration. Organic solvents can also be used in the method of making PPP to produce PPPs having variable but reproducible physical properties.

公开(公告)号：US5801047A

公开(公告)日：1998-09-01

申请号：US471993

申请日：1995-06-06

申请人： Guy Richard della-Cioppa ,  Stephen John Garger, Jr. ,  Richard Barry Holtz ,  Michael Jay McCulloch ,  Genadie Gleb Sverlow

发明人： Guy Richard della-Cioppa ,  Stephen John Garger, Jr. ,  Richard Barry Holtz ,  Michael Jay McCulloch ,  Genadie Gleb Sverlow

IPC分类号： C12N15/09 ,  C08G61/00 ,  C08H99/00 ,  C12N20060101 ,  C12N1/21 ,  C12N9/02 ,  C12N9/96 ,  C12N15/53 ,  C12P20060101 ,  C12P7/00 ,  C12P17/00 ,  C12P21/08 ,  C12R1/48 ,  C12N1/20

CPC分类号： C12Y110/03001 ,  C08G61/00 ,  C12N9/0059 ,  C12N9/0071 ,  C12P17/00 ,  C12Y114/18001

摘要： A process for the in vitro production of chemically modified polyphenolic polymer (PPP). First, stable, highly active extracellular tyrosinase is produced from genetically transformed microorganism such as Streptomyces antibioticus. The tyrosinase is then incubated with a reaction substrate such as 1-tyrosine, hydrolyzed protein, or an oligopeptide in combination with 1-tyrosine. The ratio of the oligopeptide/tyrosine combination as well as variation in the concentration of tyrosinase can be used to modify the color, the molecular size, and the spectral absorbance properties of the PPP produced. Alternatively, or additionally, oxidants such as hydrogen peroxide or hypochlorite can be used to modify the color of the PPP, regardless of the method used to produce the PPP, and the PPP can subsequently be fractionated using molecular weight cut-off ultrafiltration. Organic solvents can also be used in the method of making PPP to produce PPPs having variable but reproducible physical properties.

摘要翻译： 一种体外生产化学改性多酚聚合物（PPP）的方法。 首先，稳定的，高活性的细胞外酪氨酸酶由遗传转化的微生物如抗链霉菌（Streptomyces antibioticus）产生。 然后将酪氨酸酶与1-酪氨酸，水解蛋白质或寡肽与1-酪氨酸组合的反应底物孵育。 寡肽/酪氨酸组合的比例以及酪氨酸酶浓度的变化可用于改变生产的PPP的颜色，分子大小和光谱吸收性质。 或者或另外，可以使用氧化剂如过氧化氢或次氯酸盐来改变PPP的颜色，而不管用于生产PPP的方法如何，并且随后可以使用分子量截止超滤对PPP进行分级。 有机溶剂也可用于制备PPP以产生具有可变但可再现的物理性质的PPP的方法。

公开(公告)号：US06274358B1

公开(公告)日：2001-08-14

申请号：US08218165

申请日：1994-03-25

申请人： Richard Barry Holtz ,  Michael Jay McCulloch ,  Stephen John Garger ,  Richard King Teague ,  Harriet Flannery Phillips

发明人： Richard Barry Holtz ,  Michael Jay McCulloch ,  Stephen John Garger ,  Richard King Teague ,  Harriet Flannery Phillips

IPC分类号： C12P704

CPC分类号： C12P7/04 ,  C12P7/24

摘要： Green note compound, such as cis-3-hexen-1-ol, is provided by subjecting linolenic acid and a fresh watermelon foliage to shearing in the presence of an aqueous liquid and yeast. Enzymes within the plant material (i.e., lipoxygenase and hydroperoxide lyase) and the yeast act to cause the linolenic acid to be converted to green note alcohol at a relatively high yield. Green note compound can be provided naturally using a continuous or batch process.

摘要翻译： 通过在含水液体和酵母的存在下使亚麻酸和新鲜的西瓜叶片进行剪切来提供绿色音符化合物，例如顺式-3-己烯-1-醇。 植物材料内的酶（即，脂氧合酶和氢过氧化物裂解酶）和酵母作用使得亚麻酸以相当高的产率转化成绿色音符酒。 可以使用连续或间歇方法自然地提供绿色音符化合物。

公开(公告)号：US5934012A

公开(公告)日：1999-08-10

申请号：US344243

申请日：1994-11-23

申请人： Richard Barry Holtz ,  Michael J. McCulloch

发明人： Richard Barry Holtz ,  Michael J. McCulloch

IPC分类号： A01G1/04 ,  A23L1/28 ,  C12M1/08

CPC分类号： C12M29/08 ,  A01G1/04 ,  A23L31/00

摘要： This invention relates to a submerged fermentation process for producing high biomass levels of mushrooms mycelia in liquid media suitable for semi-continuous or continuous mushroom spawn production. The process provides a sterile, log phase inoculum for a solid substrate that, when based on biomass, exceeds normal inoculation levels by several thousand fold mycelia substrate production. The liquid inoculum so produced can be aseptically transferred to bulk sterilizer to inoculate a sterilizer grain or sawdust substrate for commercial mushroom production. The liquid inoculum may also be inoculated directly onto the mushroom compost. This invention further relates to microcapsules used to enhance the fermentation process and the equipment used to conduct such process.

摘要翻译： 本发明涉及用于在适于半连续或连续的蘑菇产卵生产的液体培养基中产生高生物量的蘑菇菌丝体的浸没发酵方法。 该方法为固体底物提供无菌的对数期接种物，当基于生物量时，超过正常接种水平达数千倍的菌丝体底物生产。 如此生产的液体接种物可以无菌转移到大容量灭菌器中以接种用于商业蘑菇生产的杀菌剂颗粒或锯屑底物。 液体接种物也可以直接接种在蘑菇堆肥上。 本发明还涉及用于增强发酵过程的微胶囊和用于进行该方法的设备。