公开(公告)号：US20150125506A1

公开(公告)日：2015-05-07

申请号：US14583838

申请日：2014-12-29

Applicant: HADASIT MEDICAL RESEARCH SERVICES AND DEVELOPMENT LTD.

Inventor： Masha IDELSON ,  Ruslana ALPER-PINUS ,  Alex OBOLENSKY ,  Eyal BANIN ,  Benjamin Eithan REUBINOFF

IPC: A61K35/30 ,  C12N5/079 ,  A61K9/00

CPC classification number: A61K35/30 ,  A61K9/0051 ,  A61K35/44 ,  A61K35/48 ,  C12N5/0621 ,  C12N5/0696 ,  C12N2500/38 ,  C12N2500/90 ,  C12N2500/99 ,  C12N2501/115 ,  C12N2501/15 ,  C12N2501/155 ,  C12N2501/16 ,  C12N2501/999 ,  C12N2502/13 ,  C12N2506/02 ,  C12N2533/52

Abstract: The present invention concerns RPE cells obtainable by directed differentiation from stem cell, particularly, human stem cells. It has been specifically found that culturing stem cells in the presence of one or more member of the TGFβ superfamily, such as Activin A) induced directed differentiation into mature and functional RPE cells. This was evidenced by the expression of markers specific to mature RPE cells, including MiTF-A, RPE65 or Bestrophin). In accordance with one particular embodiment, the cells are a priori cultured with nicotinamide (NA) which was found to augment the cells' response to the inductive effect of the one or more member of the TGFβ superfamily. The invention also provides methods of performing the directed differentiation, as well as methods for use of the resulting RPE cells.

公开(公告)号：US20150118749A1

公开(公告)日：2015-04-30

申请号：US14583848

申请日：2014-12-29

Applicant: HADASIT MEDICAL RESEARCH SERVICES AND DEVELOPMENT LTD.

Inventor： Masha IDELSON ,  Ruslana ALPER-PINUS ,  Alex OBOLENSKY ,  Eyal BANIN ,  Benjamin Eithan REUBINOFF

IPC: C12N5/079 ,  A61K35/30

CPC classification number: A61K35/30 ,  A61K9/0051 ,  A61K35/44 ,  A61K35/48 ,  C12N5/0621 ,  C12N5/0696 ,  C12N2500/38 ,  C12N2500/90 ,  C12N2500/99 ,  C12N2501/115 ,  C12N2501/15 ,  C12N2501/155 ,  C12N2501/16 ,  C12N2501/999 ,  C12N2502/13 ,  C12N2506/02 ,  C12N2533/52

Abstract: The present invention concerns RPE cells obtainable by directed differentiation from stem cell, particularly, human stem cells. It has been specifically found that culturing stem cells in the presence of one or more member of the TGFβ superfamily, such as Activin A) induced directed differentiation into mature and functional RPE cells. This was evidenced by the expression of markers specific to mature RPE cells, including MiTF-A, RPE65 or Bestrophin). In accordance with one particular embodiment, the cells are a priori cultured with nicotinamide (NA) which was found to augment the cells' response to the inductive effect of the one or more member of the TGFβ superfamily. The invention also provides methods of performing the directed differentiation, as well as methods for use of the resulting RPE cells.

Abstract translation: 本发明涉及通过从干细胞，特别是人干细胞的定向分化而获得的RPE细胞。 已经具体地发现，在一种或多种TGFβb的成员的存在下培养干细胞; 超家族，如激活素A）诱导定向分化成成熟和功能性RPE细胞。 这证明了成熟RPE细胞特异性标志物的表达，包括MiTF-A，RPE65或Bestrophin）。 根据一个具体实施方案，细胞是先前用烟酰胺（NA）培养的，其被发现增加细胞对TGFβ的一种或多种成员的诱导作用的反应; 超家族 本发明还提供了进行定向分化的方法以及使用所得RPE细胞的方法。