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1.发明申请公开(公告)号：US20150125506A1
公开(公告)日：2015-05-07
申请号：US14583838
申请日：2014-12-29
Inventor： Masha IDELSON , Ruslana ALPER-PINUS , Alex OBOLENSKY , Eyal BANIN , Benjamin Eithan REUBINOFF
CPC classification number: A61K35/30 , A61K9/0051 , A61K35/44 , A61K35/48 , C12N5/0621 , C12N5/0696 , C12N2500/38 , C12N2500/90 , C12N2500/99 , C12N2501/115 , C12N2501/15 , C12N2501/155 , C12N2501/16 , C12N2501/999 , C12N2502/13 , C12N2506/02 , C12N2533/52
Abstract: The present invention concerns RPE cells obtainable by directed differentiation from stem cell, particularly, human stem cells. It has been specifically found that culturing stem cells in the presence of one or more member of the TGFβ superfamily, such as Activin A) induced directed differentiation into mature and functional RPE cells. This was evidenced by the expression of markers specific to mature RPE cells, including MiTF-A, RPE65 or Bestrophin). In accordance with one particular embodiment, the cells are a priori cultured with nicotinamide (NA) which was found to augment the cells' response to the inductive effect of the one or more member of the TGFβ superfamily. The invention also provides methods of performing the directed differentiation, as well as methods for use of the resulting RPE cells.
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公开(公告)号：US20220233477A1
公开(公告)日：2022-07-28
申请号：US17618541
申请日：2020-06-11
Inventor： Benjamin Eithan REUBINOFF , Etti BEN-SHUSHAN
IPC: A61K31/166 , C12N5/079 , A61P9/10 , A61P37/06 , A61P25/28
Abstract: A method of generating human mature oligodendrocytes is disclosed. The method comprises contacting a cell population which comprises human pre-oligodendrocytes with an inhibitor of the MAPK/ERK pathway under conditions that allow the pre-oligodendrocytes to differentiate into mature oligodendrocytes. Use of the MAPK/ERK pathway inhibitor for treating diseases is also disclosed.
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公开(公告)号：US20180216064A1
公开(公告)日：2018-08-02
申请号：US15748541
申请日：2016-07-28
Inventor： Benjamin Eithan REUBINOFF , Orna SINGER
CPC classification number: C12N5/0621 , A61K9/0051 , A61K35/30 , A61P27/02 , C12N2500/38 , C12N2501/115 , C12N2501/15 , C12N2502/13 , C12N2506/02 , C12N2533/52 , C12N2533/54
Abstract: A method of generating retinal pigment epithelial (RPE) cells is disclosed. The method comprises: (a) culturing human pluripotent stem cells in a human feeder cell-conditioned medium to obtain a cultured population of human pluripotent stem cells; (b) culturing said cultured population of human pluripotent stem cells in a medium comprising a differentiating agent to obtain differentiating cells; and (c) culturing said differentiating cells in a medium comprising one or more members of the TGFβ superfamily.
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公开(公告)号：US20170199190A1
公开(公告)日：2017-07-13
申请号：US15470926
申请日：2017-03-28
Inventor： Benjamin Eithan REUBINOFF , Nurit YACHIMOVICH-COHEN , Limor MATZRAFI
IPC: G01N33/569 , C12N5/079 , G01N33/58
CPC classification number: G01N33/56966 , C12N5/0621 , C12N2501/15 , C12N2501/16 , C12N2506/45 , C12N2509/00 , G01N21/6486 , G01N33/52 , G01N33/582 , G01N2021/4704 , G01N2333/46
Abstract: A method of selecting retinal pigmented epithelial (RPE) cells from a mixed population of cells is disclosed. The method comprises: (a) analyzing the cells of the mixed population of cells for at least one of the following parameters: (i) cells which autofluorescence above a predetermined threshold; (ii) cells which express CD81 above a predetermined threshold; and (iii) cells which scatter light perpendicular to a laser beam above a predetermined threshold; and (b) selecting cells which are positive for at least one of the parameters, thereby sorting RPE cells from a mixed population of cells.
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公开(公告)号：US20220098551A1
公开(公告)日：2022-03-31
申请号：US17547863
申请日：2021-12-10
Inventor： Benjamin Eithan REUBINOFF , Orna SINGER
Abstract: A method of generating retinal pigment epithelial (RPE) cells is disclosed. The method comprises: (a) culturing human pluripotent stem cells in a human feeder cell-conditioned medium to obtain a cultured population of human pluripotent stem cells; (b) culturing said cultured population of human pluripotent stem cells in a medium comprising a differentiating agent to obtain differentiating cells; and (c) culturing said differentiating cells in a medium comprising one or more members of the TGFβ superfamily.
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Patent Agency Ranking
6.发明申请RETINAL PIGMENT EPITHELIAL CELLS DIFFERENTIATED FROM EMBRYONIC STEM CELLS WITH NICOTINAMIDE AND ACTIVIN A 审中-公开来自具有尼古丁和激活素A的胚胎干细胞分化的后皮肤上皮细胞公开(公告)号：US20150118749A1
公开(公告)日：2015-04-30
申请号：US14583848
申请日：2014-12-29
Inventor： Masha IDELSON , Ruslana ALPER-PINUS , Alex OBOLENSKY , Eyal BANIN , Benjamin Eithan REUBINOFF
CPC classification number: A61K35/30 , A61K9/0051 , A61K35/44 , A61K35/48 , C12N5/0621 , C12N5/0696 , C12N2500/38 , C12N2500/90 , C12N2500/99 , C12N2501/115 , C12N2501/15 , C12N2501/155 , C12N2501/16 , C12N2501/999 , C12N2502/13 , C12N2506/02 , C12N2533/52
Abstract: The present invention concerns RPE cells obtainable by directed differentiation from stem cell, particularly, human stem cells. It has been specifically found that culturing stem cells in the presence of one or more member of the TGFβ superfamily, such as Activin A) induced directed differentiation into mature and functional RPE cells. This was evidenced by the expression of markers specific to mature RPE cells, including MiTF-A, RPE65 or Bestrophin). In accordance with one particular embodiment, the cells are a priori cultured with nicotinamide (NA) which was found to augment the cells' response to the inductive effect of the one or more member of the TGFβ superfamily. The invention also provides methods of performing the directed differentiation, as well as methods for use of the resulting RPE cells.
Abstract translation: 本发明涉及通过从干细胞，特别是人干细胞的定向分化而获得的RPE细胞。 已经具体地发现，在一种或多种TGFβb的成员的存在下培养干细胞; 超家族，如激活素A）诱导定向分化成成熟和功能性RPE细胞。 这证明了成熟RPE细胞特异性标志物的表达，包括MiTF-A，RPE65或Bestrophin）。 根据一个具体实施方案，细胞是先前用烟酰胺（NA）培养的，其被发现增加细胞对TGFβ的一种或多种成员的诱导作用的反应; 超家族 本发明还提供了进行定向分化的方法以及使用所得RPE细胞的方法。
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公开(公告)号：US20220213445A1
公开(公告)日：2022-07-07
申请号：US17613137
申请日：2020-05-22
Inventor： Benjamin Eithan REUBINOFF , Michal GROPP
IPC: C12N5/074 , C12N5/0735 , C12N5/073
Abstract: A culture of human pluripotent stem cells (hPSCs) is disclosed. In the culture, more than 50% of the hPSCs are formative hPSCs and are capable of renewing. Uses thereof are also disclosed.
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公开(公告)号：US20190137482A1
公开(公告)日：2019-05-09
申请号：US16242023
申请日：2019-01-08
Inventor： Talya MORDECHAI DANIEL , Ofer WISER , Benjamin Eithan REUBINOFF
IPC: G01N33/50
Abstract: A method of determining whether an agent is a neuroeffector is disclosed. The method comprises: (a) labeling dopaminergic neurons which are comprised in a mixed population of cells with a fluorescent dopamine analog; (b) measuring a level of fluorescence in the mixed population of cells; (c) exposing the mixed population of cells to the agent; (d) remeasuring a level of fluorescence in the mixed population of cells, wherein a change in the level of fluorescence is indicative of the substance being a neuroeffector.
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公开(公告)号：US20180312805A1
公开(公告)日：2018-11-01
申请号：US15771046
申请日：2016-10-26
Inventor： Benjamin Eithan REUBINOFF , Orna SINGER , Osnat BOHANA-KASHTAN , Ofer WISER
IPC: C12N5/079 , C12N5/0735 , C12N5/00
CPC classification number: C12N5/0621 , C12N2501/15 , C12N2501/16 , C12N2506/02 , C12N2533/52
Abstract: A method of generating retinal pigment epithelium cells is disclosed. Cell populations comprising same and uses thereof are also disclosed.
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公开(公告)号：US20150377864A1
公开(公告)日：2015-12-31
申请号：US14766784
申请日：2014-02-12
Inventor： Talya MORDECHAI DANIEL , Ofer WISER , Benjamin Eithan REUBINOFF
IPC: G01N33/50
CPC classification number: G01N33/5058 , G01N33/502 , G01N2333/70571
Abstract: A method of determining whether an agent is a neuroeffector is disclosed. The method comprises: (a) labeling dopaminergic neurons which are comprised in a mixed population of cells with a fluorescent dopamine analog; (b) measuring a level of fluorescence in the mixed population of cells; (c) exposing the mixed population of cells to the agent; (d) remeasuring a level of fluorescence in the mixed population of cells, wherein a change in the level of fluorescence is indicative of the substance being a neuroeffector.
Abstract translation: 公开了一种确定药剂是否是神经递质的方法。 该方法包括：（a）将含有荧光多巴胺类似物的混合细胞群中的多巴胺能神经元标记; （b）测量混合细胞群体中的荧光水平; （c）将混合的细胞群暴露于所述试剂; （d）重新测量混合细胞群体中的荧光水平，其中荧光水平的变化指示物质是神经递质。
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