公开(公告)号：US20230273218A1

公开(公告)日：2023-08-31

申请号：US18027240

申请日：2021-06-10

Applicant: HANGZHOU ZHEDA DIXUN BIOLOGICAL GENE ENGINEERING CO., LTD.

Inventor： Zhoujie WU ,  Yi LIU ,  Shandong WU ,  Huahao SHEN ,  Shanshan CHEN ,  Shaochang WU ,  Yifei WANG ,  Mingzhi ZHU ,  Meijie WANG ,  Chuhan CHEN

IPC: G01N33/68 ,  G01N33/545 ,  G01N33/577 ,  G01N33/58

CPC classification number: G01N33/6854 ,  G01N33/545 ,  G01N33/577 ,  G01N33/582 ,  G01N33/6869 ,  G01N33/6866 ,  G01N2800/60

Abstract: The present disclosure relates to a kit for quantitative detection using a fluorescent microarray, and belongs to the technical field of protein detection. The kit of the present disclosure includes a detection plate and a detection antibody coupled with fluorescent microspheres, where the detection plate is provided with a plurality of reaction chambers; the reaction chamber is provided with an opening, and an inner bottom surface of the reaction chamber is provided with a plurality of detection sites that are arranged side by side along a length direction of the reaction chamber at an interval. The kit of the present disclosure may detect allergen-specific IgE, IgG and IgA with high sensitivity, as well as rapidly and quantitatively detect an allergen-specific antibody IgE, IgG and IgA concentration in human serum or plasma, and may screen dozens of allergens at a time.

公开(公告)号：US20240158856A1

公开(公告)日：2024-05-16

申请号：US18282157

申请日：2021-08-04

Applicant: HANGZHOU ZHEDA DIXUN BIOLOGICAL GENE ENGINEERING CO., LTD

Inventor： Shandong WU ,  Yi LIU ,  Zhoujie WU ,  Xuehan JIANG ,  Jiping WANG ,  Meijie WANG ,  Xukai YANG

IPC: C12Q1/6883

CPC classification number: C12Q1/6883 ,  C12Q2600/158 ,  C12Q2600/166

Abstract: The present disclosure provides a primer probe set for human histamine receptor HRH1 mRNA detection, a kit and a detection method, and relates to the technical field of biological detection. In the present disclosure, the primer probe set includes a HRH1-F, a HRH1-R and a probe H1-Probe; where the HRH1-F has a nucleotide sequence shown in SEQ ID NO. 1, the HRH1-R has a nucleotide sequence shown in SEQ ID NO. 2, and the probe H1-Probe has a nucleotide sequence shown in SEQ ID NO. 3. The present disclosure provides a kit including the primer probe set and a detection method. An expression level of the HRH1 mRNA can be detected using an RNA one-step method.

公开(公告)号：US20240150832A1

公开(公告)日：2024-05-09

申请号：US18282159

申请日：2021-08-04

Applicant: HANGZHOU ZHEDA DIXUN BIOLOGICAL GENE ENGINEERING CO., LTD

Inventor： Shandong WU ,  Yi LIU ,  Zhoujie WU ,  Xuehan JIANG ,  Xukai YANG ,  Meijie WANG ,  Weiyue CAI

IPC: C12Q1/6876

CPC classification number: C12Q1/6876 ,  C12Q1/686 ,  C12Q2600/158

Abstract: The present disclosure provides a reagent for detecting an expression level of a human histamine receptor HRH4 mRNA, a kit and a detection method. In the present disclosure, the reagent includes a specific primer and a probe for a human histamine receptor HRH4, the specific primer includes an HRH4-F and an HRH4-R, and the probe includes an H4-Probe; and the HRH4-F has a nucleotide sequence shown in SEQ ID NO. 1, the HRH4-R has a nucleotide sequence shown in SEQ ID NO. 2 and the H4-Probe has a nucleotide sequence shown in SEQ ID NO. 3. In the present disclosure, a kit for one-step detection and a detection method based on the reagent are prepared, and the expression level of the HRH4 mRNA can be one-step quantitatively detected with simple operation and short detection time.