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Patent Agency Ranking
公开(公告)号：US3769168A
公开(公告)日：1973-10-30
申请号：US3769168D
申请日：1971-06-11
Applicant: HAYASHIBARA CO
Inventor： MASUDA K
CPC classification number: C12N9/2425 , Y10S435/815
Abstract: The present procedure concerns a process for the purification of beta-amylases comprising treating a crude beta-amylase solution derived from wheat bran, soy bean or fresh sweet potato with an adsorbent such as bentonite, acidic clay, kaolinite and activated clay at a pH lower than 5.0 to adsorb the beta-amylases, washing out the adsorbent with water, and then eluting the adsorbed betaamylases with a solution with an ionic strength over 0.5 Mu and a pH exceeding 5.0.
Abstract translation: 本方法涉及纯化β-淀粉酶的方法，包括用pH低的吸附剂如膨润土，酸性粘土，高岭土和活性粘土处理来自麦麸，大豆或新鲜甘薯的粗β-淀粉酶溶液 超过5.0以吸附β-淀粉酶，用水洗涤吸附剂，然后用离子强度超过0.5μm并且pH超过5.0的溶液洗脱吸附的β-淀粉酶。
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公开(公告)号：US3795584A
公开(公告)日：1974-03-05
申请号：US3795584D
申请日：1971-09-03
Applicant: HAYASHIBARA CO
Inventor： MITSUHASHI M , HIRAO M , SUGIMOTO K , MASUDA K , SHIOSAKA M , YOSHIDA M , YOKOBAYASHI Y , YUEN S
CPC classification number: C12P19/22 , C13K7/00 , Y10S435/874
Abstract: ACCORDING TO THE PRESENT INVENTION, STARCH IS FIRST LIQUIFIED WITH ACID OR ENZYME, THE PH OF THE RESULTING LIQUIFIED STARCH IS ADJUSTED TO ABOUT 6, THE LIQUIFIED STARCH IS SACCHARIFIED BY THE USE OF BETA-AMYLASE AND ALPHA-1, 6GLUCO-SIDASE OR IN ADDITION ALPHA-AMYLASE DURING THE SACCHARIFYING STEP TO PRODUCE A MALTOSE WITH PURITY OF ABOUT 99%.
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公开(公告)号：US3766014A
公开(公告)日：1973-10-16
申请号：US3766014D
申请日：1971-04-23
Applicant: HAYASHIBARA CO
Inventor： MASUDA K , SUGIMOTO K
CPC classification number: C12N9/2451 , C12Y302/01033 , Y10S435/816 , Y10S435/852
Abstract: The production of alpha-1,6-glucosidase by the cultivation of a strain of Klebsiella pneumoniae ATCC 13883, Klebsiella rhinoscleromatis ATCC 13884, Klebsiella ozaenae ATCC 13885, Klebsiella edwardsii ATCC 13886 or Klebsiella rubiacearum ATCC 15574 on media consisting of carbon sources, nitrogen sources, inorganic salts and others under aerating and agitating conditions thereby resulting in accumulation of alpha-1,6glucosidase on the media and the separation of the alpha-1,6glucosidase from the media following the purification of the alpha-1,6-glucosidase.
Abstract translation: 通过在由碳源，氮源组成的培养基上培养肺炎克雷伯氏杆菌ATCC 13883，克雷伯杆菌ATCC 13884，克雷伯杆菌ATCC 13885，克雷伯氏菌ATCC 13886或克雷伯氏菌（Krebsiella rubiacearum）ATCC 15574的菌株生产α-1,6-糖苷酶 ，无机盐和其它物质，从而导致在介质上积累α-1,6-葡糖苷酶并且在纯化α-1,6之后从培养基中分离α-1,6-糖苷酶 葡萄糖苷酶。
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公开(公告)号：US3723253A
公开(公告)日：1973-03-27
申请号：US3723253D
申请日：1969-07-08
Applicant: HAYASHIBARA CO
Inventor： YOKOBAYASHI Y , MASUDA K
CPC classification number: C12N9/2451 , C12Y302/01033 , Y10S435/828 , Y10S435/874
Abstract: THE PRODUCTION OF ALPHA-1,6-GLUCOSIDASE BY THE CULTURE OF AN ALPHA-1,6-GLUCOSIDASE PRODUCTIVE BACTERIUM IS CARRIED OUT BY USING A CULTURE MEDIUM CONTAINING STARCH HYDROLYZATE AS A CARBON SOURCE AND A MEMBER SELECTED FROM THE GROUP CONSISTING OF SOYBEAN CAKE HYDEOLYZATE AND A MIXTURE THEREOF WITH AN AMMONIUM SALT AS A NITROGEN SOURCE.
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公开(公告)号：US3827940A
公开(公告)日：1974-08-06
申请号：US23757872
申请日：1972-03-23
Applicant: HAYASHIBARA CO
Inventor： SUGIMOTO K , HIRAO M , MASUDA K
CPC classification number: C12N9/2451 , C12Y302/01033 , Y10S435/853 , Y10S435/855 , Y10S435/857 , Y10S435/872
Abstract: A-1,6-GLUCOSIDASES WHICH ARE CAPABLE OF SELECTIVLEY HYDROLYZING THE ALPHA-1,6-GLUCOSIDE BONDES OF STARCH AND WHICH HAVE HIGH HEAT RESISTANCE AND ACTIVITY ARE PRODUCED FROM STRAINS OF BACTERIA BELONGING TO THE GENUS ARGOBACTERIUM. AZOTOBACTER, BACILLUS, MYCOBACTEIUM, NOCARDIA, LEUCONOSTOC, MICROCOCCUS, MYCOBACTERIUM, NORCADIA, PEDIOCOCCUS, SARCINA, SERRATIA, STPHYLOCOCCUS AND STREPTOCOCCUS.
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