公开(公告)号：US20240319211A1

公开(公告)日：2024-09-26

申请号：US18576005

申请日：2023-05-08

Applicant: HEFEI NOVA-MS BIOTECH MEDICAL EQUIPMENT CO., LTD

Inventor： Jun PENG ,  Zhenzuo JIANG

IPC: G01N33/74 ,  B01D61/14 ,  B01D61/24 ,  G01N33/68

CPC classification number: G01N33/74 ,  B01D61/145 ,  B01D61/243 ,  G01N33/6848

Abstract: The present invention belongs to the field of free components detection technologies. For sample preparation of free components, although ultrafiltration is efficient and fast, the temperature is not easy to control which result in the measurement results have a large deviation. Conversely, the temperature is easy to control and measurement results are more precise when equilibrium dialysis is used. However, a long time consumed gives rise to equilibrium dialysis cannot meet the requirement for quickly analyzing clinical biological samples. Therefore, this application provides a content conversion method of free components by transforming ultrafiltration to equilibrium dialysis. Free components are separated from samples by ultrafiltration to obtain first concentration. Meanwhile, free components are separated from the samples by equilibrium dialysis to obtain second concentration. A linear equation is established based on the first concentration and the second concentration, which can meet both requirements of high detection accuracy and short time consuming.