公开(公告)号：US20210388422A1

公开(公告)日：2021-12-16

申请号：US17323311

申请日：2021-05-18

Applicant: Hitachi, Ltd.

Inventor： Masataka SHIRAI ,  Hideki KAMBARA ,  Kiyomi TANIGUCHI ,  Maiko TANABE

IPC: C12Q1/6837 ,  C12N15/10

Abstract: The present invention relates to a method, a device, and an apparatus for analyzing the expression of a gene in single cells. Specifically, the present invention relates to: a device for gene expression analysis, characterized by including a support, in which a nucleic acid probe having a test nucleic acid capture sequence and a known sequence, and further containing a cell recognition tag sequence which differs depending on the difference in position on the surface of the support or in the vicinity of the surface thereof, and a common primer sequence having a known sequence is two-dimensionally distributed and immobilized on the surface of the support or in the vicinity of the surface thereof; and a method and an apparatus using the device for gene expression analysis.

公开(公告)号：US20150302042A1

公开(公告)日：2015-10-22

申请号：US14443118

申请日：2012-11-20

Applicant: HITACHI, LTD.

Inventor： Masataka SHIRAI ,  Hideki KAMBARA ,  Kiyomi TANIGUCHI

IPC: G06F17/30 ,  G06F19/28

CPC classification number: G06F16/2365 ,  G06F16/285 ,  G16B40/00 ,  G16B50/00

Abstract: The present invention aims to provide a data analysis apparatus capable of clustering appropriately even when there is an exceptional datum resulted from an experimental error and the like. In the data analysis apparatus according to the invention, a cluster range parameter for stretching a cluster boundary is determined in advance according to the range of an experimental error which an experimental error datum describes. In the process of clustering, an exceptional datum which does not belong to any cluster is determined to belong to a cluster when an area at a distance determined by the cluster range parameter from the exceptional datum is contained in the cluster, and the exceptional datum is determined to form an independent cluster when even the area at the distance is not contained in any cluster (see FIG. 7).

Abstract translation: 本发明的目的在于提供即使在存在由实验误差等引起的异常原理的情况下也能适当聚类的数据分析装置。 在根据本发明的数据分析装置中，根据实验误差基准描述的实验误差的范围，预先确定用于拉伸簇边界的簇范围参数。 在聚类过程中，当集群中包含由特殊基准的聚类范围参数确定的距离范围内的区域被确定为属于集群时，不属于任何集群的特殊数据，并且异常数据为 确定为即使在距离上的区域不包含在任何集群中时形成独立的集群（参见图7）。

公开(公告)号：US20160010078A1

公开(公告)日：2016-01-14

申请号：US14771339

申请日：2013-03-12

Applicant: HITACHI, LTD.

Inventor： Masataka SHIRAI ,  Hideki KAMBARA ,  Kiyomi TANIGUCHI ,  Maiko TANABE

IPC: C12N15/10

CPC classification number: C12N15/1003 ,  B01L3/502761 ,  B01L7/52 ,  B01L2200/0631 ,  B01L2200/0668 ,  B01L2200/10 ,  B01L2300/0636 ,  B01L2300/0819 ,  B01L2300/0851 ,  B01L2300/0864 ,  B01L2300/1805 ,  B01L2400/0421 ,  C12N15/1096 ,  C12Q1/6874 ,  C12Q2539/10

Abstract: In order to conduct gene expression analysis of a number of genes in a number of cells, it has been necessary to separate cells, extract genes therefrom, amplify nucleic acids, and perform sequence analysis. However, separation of cells imposes damages on the cells, and it requires the use of an expensive system. Gene expression analysis in each cell can be carried out with high accuracy by arranging a pair of structures comprising a cell trapping section and a nucleic acid trapping section in a vertical direction to extract individual genes in relevant cells, synthesizing cDNA in the nucleic acid trapping section, amplifying nucleic acids, and analyzing the sequences using a next-generation sequencer.

Abstract translation: 为了对许多细胞中的许多基因进行基因表达分析，必须分离细胞，从中提取基因，扩增核酸并进行序列分析。 然而，细胞的分离对细胞造成损害，并且需要使用昂贵的系统。 通过在垂直方向布置包含细胞捕获部分和核酸捕获部分的一对结构以提取相关细胞中的单个基因，可以高精度地进行每个细胞中的基因表达分析，在核酸捕获部分中合成cDNA ，扩增核酸，并使用下一代测序仪分析序列。