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    FUNCTIONAL SEQUENCE SELECTION METHOD AND FUNCTIONAL SEQUENCE SELECTION SYSTEM
    1.
    发明申请
    FUNCTIONAL SEQUENCE SELECTION METHOD AND FUNCTIONAL SEQUENCE SELECTION SYSTEM 审中-公开

    公开(公告)号：US20200327958A1

    公开(公告)日：2020-10-15

    申请号：US16824365

    申请日：2020-03-19

    Applicant: HITACHI, LTD. ,  NATIONAL UNIVERSITY CORPORATION KOBE UNIVERSITY ,  Kyoto University

    Inventor： Maiko TANABE ,  Shizu TAKEDA ,  Kiyoto ITO ,  Osamu IMAICHI ,  Kenji TSUGE ,  Michihiro ARAKI

    IPC: G16B25/10 ,  G16B30/00 ,  G16B35/20

    Abstract: A method for making a recombinant gene includes searching a database using a nucleotide sequence of a coding region, a nucleotide sequence that encodes an amino acid sequence, or an amino acid sequence, of a gene, for one or more nucleotide sequences having homology; selecting one or more nucleotide sequences other than nucleotide sequences only derived from a genome from the selected nucleotide sequences; for ones of the selected one or more nucleotide sequences comprising an upstream or downstream nucleotide sequence, analyzing whether the upstream or downstream nucleotide sequence is a functional sequence to select one or more first functional sequences; for ones of the selected one or more nucleotide sequences comprising no upstream or downstream nucleotide sequence, analyzing whether a gene information has any description indicating a functional sequence to select one or more second functional sequences; scoring the selected functional sequences; and selecting one or more functional sequences.

    GENE ANALYSIS SYSTEM
    4.
    发明申请
    GENE ANALYSIS SYSTEM 审中-公开

    公开(公告)号：US20180119135A1

    公开(公告)日：2018-05-03

    申请号：US15553175

    申请日：2015-04-09

    Applicant: Hitachi, Ltd.

    Inventor： Maiko TANABE ,  Masataka SHIRAI ,  Tomoyuki SAKAI

    IPC: C12N15/10

    CPC classification number: C12N15/1065 ,  C12M1/00 ,  C12N15/1096 ,  C12Q1/68 ,  C12Q1/6886 ,  C12Q2600/158 ,  C12Q2563/179

    Abstract: In order to interpret an arbitrary sequence region in many genes in many cells, it is necessary to degrade a nucleic acid into fragments and introduce a sequence that is different from one cell to another into each of the fragments. However, in the conventional configuration for analyzing many cells, there has been a problem that mixing of the degraded fragments among areas occurs before a tag sequence unique for each of the areas is introduced. The present invention provides a system for capturing a nucleic acid extracted from a cell in each of plural areas on a substrate and synthesizing a complementary DNA (cDNA) of the nucleic acid for each of the areas, wherein the system also includes a means for immediately introducing a tag sequence unique for each of the areas to the reaction product.

    CELL CULTURE METHOD AND AUTOMATIC CELL CULTURE APPARATUS
    5.
    发明申请
    CELL CULTURE METHOD AND AUTOMATIC CELL CULTURE APPARATUS 有权

    公开(公告)号：US20210284959A1

    公开(公告)日：2021-09-16

    申请号：US17162207

    申请日：2021-01-29

    Applicant: Hitachi, Ltd.

    Inventor： Yuki NIIMI ,  Hiroko HANZAWA ,  Maiko TANABE ,  Kunio OHYAMA ,  Shizu TAKEDA

    IPC: C12N5/073 ,  C12M1/36

    Abstract: A novel cell culture method for inducing differentiation of a pluripotent stem cell into trophoblast and an automatic culture apparatus therefor includes: a first step of culturing the pluripotent stem cell in a presence of a ROCK inhibitor during a first time period; a second step of culturing the pluripotent stem cell, which has been subjected to the first step, without the ROCK inhibitor during a second time period following the first time period; and a step of culturing the pluripotent stem cell, which has been subjected to the second step, in the presence of the ROCK inhibitor during a third time period following the second time period, in which the pluripotent stem cell is cultured in a state of being adhered to a cell culture substrate including a planar mesh through the first to third time periods.

    Two-Dimensional Cell Array Device and Apparatus for Gene Quantification and Sequence Analysis
    6.
    发明申请
    Two-Dimensional Cell Array Device and Apparatus for Gene Quantification and Sequence Analysis 审中-公开
    用于基因定量和序列分析的二维细胞阵列装置和装置

    公开(公告)号：US20160010078A1

    公开(公告)日：2016-01-14

    申请号：US14771339

    申请日：2013-03-12

    Applicant: HITACHI, LTD.

    Inventor： Masataka SHIRAI ,  Hideki KAMBARA ,  Kiyomi TANIGUCHI ,  Maiko TANABE

    IPC: C12N15/10

    CPC classification number: C12N15/1003 ,  B01L3/502761 ,  B01L7/52 ,  B01L2200/0631 ,  B01L2200/0668 ,  B01L2200/10 ,  B01L2300/0636 ,  B01L2300/0819 ,  B01L2300/0851 ,  B01L2300/0864 ,  B01L2300/1805 ,  B01L2400/0421 ,  C12N15/1096 ,  C12Q1/6874 ,  C12Q2539/10

    Abstract: In order to conduct gene expression analysis of a number of genes in a number of cells, it has been necessary to separate cells, extract genes therefrom, amplify nucleic acids, and perform sequence analysis. However, separation of cells imposes damages on the cells, and it requires the use of an expensive system. Gene expression analysis in each cell can be carried out with high accuracy by arranging a pair of structures comprising a cell trapping section and a nucleic acid trapping section in a vertical direction to extract individual genes in relevant cells, synthesizing cDNA in the nucleic acid trapping section, amplifying nucleic acids, and analyzing the sequences using a next-generation sequencer.

    Abstract translation: 为了对许多细胞中的许多基因进行基因表达分析，必须分离细胞，从中提取基因，扩增核酸并进行序列分析。 然而，细胞的分离对细胞造成损害，并且需要使用昂贵的系统。 通过在垂直方向布置包含细胞捕获部分和核酸捕获部分的一对结构以提取相关细胞中的单个基因，可以高精度地进行每个细胞中的基因表达分析，在核酸捕获部分中合成cDNA ，扩增核酸，并使用下一代测序仪分析序列。

    BIOLOGICAL REACTION INFORMATION PROCESSING SYSTEM AND BIOLOGICAL REACTION INFORMATION PROCESSING METHOD
    8.
    发明申请
    BIOLOGICAL REACTION INFORMATION PROCESSING SYSTEM AND BIOLOGICAL REACTION INFORMATION PROCESSING METHOD 审中-公开

    公开(公告)号：US20200303042A1

    公开(公告)日：2020-09-24

    申请号：US16816732

    申请日：2020-03-12

    Applicant: Hitachi, Ltd.

    Inventor： Taiki FUJI ,  Kiyoto ITO ,  Shiori NAKAZAWA ,  Maiko TANABE

    IPC: G16C20/30 ,  G16C20/70 ,  G05B13/04 ,  G16C20/10

    Abstract: To predict a new biological reaction by quantifying while retaining characteristics of an entire compound structure. To provide a structural characteristic amount encoding unit that includes a conversion model unit configured to convert a characteristic amount of notation information indicating chemical structures of a plurality of compounds into a dispersedly represented numerical vector having at least two or more real number values as an element using a conversion parameter, the conversion model unit converting the characteristic amount of the notation information indicating the chemical structures into a numerical vector, for each of a first compound and a second compound among the plurality of compounds, and a biological reaction characteristic vector generator configured to generate a biological reaction characteristic vector between the first compound and the second compound by performing a calculation using a numerical vector of the first compound and a numerical vector of the second compound.

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