公开(公告)号：US20240102088A1

公开(公告)日：2024-03-28

申请号：US18529759

申请日：2023-12-05

Applicant: Hitachi, Ltd.

Inventor： Junko TANAKA ,  Takahide YOKOI ,  Masao KAMAHORI ,  Yoshinobu KOHARA

IPC: C12Q1/686 ,  C12Q1/6848

CPC classification number: C12Q1/686 ,  C12Q1/6848

Abstract: The present invention is directed to provide new PCR measuring method and device. As one embodiment of the present invention, a DNA detection method for detecting DNA in a droplet being present in oil, the droplet containing the DNA and a fluorescent labeled probe, the fluorescent labeled probe being hybridized to the DNA, the method including: a first step of amplifying the DNA in the droplet by a nucleic acid amplification reaction; and a second step of measuring a melting temperature of the fluorescent labeled probe and the DNA in the droplet is provided.

公开(公告)号：US20190352699A1

公开(公告)日：2019-11-21

申请号：US16476256

申请日：2017-11-16

Applicant: HITACHI, LTD.

Inventor： Junko TANAKA ,  Takahide YOKOI ,  Masao KAMAHORI ,  Yoshinobu KOHARA

IPC: C12Q1/686 ,  C12Q1/6848

Abstract: The present invention is directed to provide new PCR measuring method and device. As one embodiment of the present invention, a DNA detection method for detecting DNA in a droplet being present in oil, the droplet containing the DNA and a fluorescent labeled probe, the fluorescent labeled probe being hybridized to the DNA, the method including: a first step of amplifying the DNA in the the droplet by a nucleic acid amplification reaction; and a second step of measuring a melting temperature of the fluorescent labeled probe and the DNA in the droplet is provided.

公开(公告)号：US20180284083A1

公开(公告)日：2018-10-04

申请号：US15764249

申请日：2015-12-09

Applicant: HITACHI, LTD.

Inventor： Masako ISHIMARU ,  Masao KAMAHORI ,  Masuyuki SUGIYAMA ,  Kazushige NISHIMURA ,  Hiroyuki SATAKE ,  Hideki HASEGAWA

IPC: G01N30/72 ,  G01N30/02

Abstract: In order to implement a high-sensitivity mass spectrometry through an improvement in solvent removal efficiency during electrospray ionization and the like, an ionization device is provided with a light guide path 28 which guides light from a light source to sample microparticles generated by a micronization device to irradiate the microparticles. A closest distance d2 between a spatial area 34 in which the sample microparticles are present and a distal end 29 of the light guide path is greater than or equal to 0.1 mm and less than or equal to 20 mm. A closest distance d1 between an area of light irradiation 35 by the light guide path and any of a sample surface, a micronization device, and a sample holding unit that is the closest is greater than or equal to 0.01 mm and less than or equal to 10 mm.

公开(公告)号：US20130274126A1

公开(公告)日：2013-10-17

申请号：US13802970

申请日：2013-03-14

Applicant: HITACHI, LTD.

Inventor： Yu ISHIGE ,  Masao KAMAHORI

IPC: C12Q1/04

CPC classification number: C12Q1/04

Abstract: Provided is a device that can detect cells or bacteria in units of a single cell or bacterium, and can further measure the amounts of activity of cells or bacteria or responses of the cells or bacteria to drugs in units of a single cell or bacterium. A plurality of partitioned regions each having about the same size as a cell or a bacterium is provided, and a plurality of types of electrical sensors 201 and 202 are arranged in each partitioned region.

Abstract translation: 提供了可以以单个细胞或细菌为单位检测细胞或细菌的装置，并且可以以单个细胞或细菌为单位进一步测量细胞或细菌的活性或细胞或细菌对药物的反应的量。 提供了与细胞或细菌具有大致相同尺寸的多个分割区域，并且在每个分割区域中布置有多种类型的电传感器201和202。