公开(公告)号：US20190039046A1

公开(公告)日：2019-02-07

申请号：US16077577

申请日：2017-01-13

Applicant: HITACHI, LTD.

Inventor： Masashi MARUYAMA ,  Nanae YAMASHITA ,  Hiroshi YOSHIDA ,  Keisuke SHIBUYA

IPC: B01J20/281 ,  B01J20/32 ,  B01D15/38 ,  C07K1/22

Abstract: The present invention has a subject of desorbing a target substance under mild elusion conditions in affinity purification using a low molecular weight ligand. In the present invention, a polar auxiliary group is introduced to a site adjacent to the low molecular weight ligand to perturb a pKa value of a functional group in the low-molecular-weight ligand, thereby ionizing the low molecular weight ligand under mild pH conditions.