公开(公告)号：US11708397B2

公开(公告)日：2023-07-25

申请号：US16768153

申请日：2018-12-31

申请人： HOWARD HUGHES MEDICAL INSTITUTE

发明人： Eric R Schreiter ,  Luke D. Lavis ,  Claire Deo ,  Hersh Bhargava ,  Ahmed Abdelfattah

IPC分类号： C07K14/47 ,  G01N33/58 ,  G01N33/84 ,  G01N21/77 ,  A61K49/00

CPC分类号： C07K14/4728 ,  A61K49/0004 ,  A61K49/0041 ,  A61K49/0056 ,  G01N21/77 ,  G01N33/582 ,  G01N33/84 ,  C07K2319/20 ,  C07K2319/60 ,  G01N2021/7786 ,  G01N2333/4727

摘要： A chemigenetic calcium indicator and a method of measuring calcium are provided. The chemigenetic calcium indicator includes a calcium-binding protein domain attached to a ligand binding protein domain. The method of measuring calcium includes administering a chemigenetic calcium indicator to a subject and determining changes in fluorescence, the chemigenetic calcium indicator including a ligand binding protein domain having a calcium-binding protein domain and a dye-ligand conjugate attached thereto.