公开(公告)号：US20140235460A1

公开(公告)日：2014-08-21

申请号：US14266884

申请日：2014-05-01

Applicant: HTG Molecular Diagnostics, Inc.

Inventor： Debrah Thompson ,  Bruce E. Seligmann ,  Debra A. Gordon

IPC: C12Q1/68

CPC classification number: C12Q1/6853 ,  C12Q1/6869 ,  C12Q1/6874 ,  C12Q2561/108

Abstract: The present disclosure provides an improvement to quantitative Nuclease Protection Assay (qNPA) and quantitative Nuclease Protection Sequencing (qNPS) methods. The disclosed methods use nuclease protection probes (NPPs) that include 5′-end and/or 3-end flanking sequences, which provide a universal hybridization and/or amplification sequence. The disclosed methods can be used to sequence or detect target nucleic acid molecules, such as those present in fixed or insoluble samples.

Abstract translation: 本公开提供了定量核酸酶保护测定（qNPA）和定量核酸酶保护测序（qNPS）方法的改进。 所公开的方法使用包括5'端和/或3端侧翼序列的核酸酶保护探针（NPP），其提供通用杂交和/或扩增序列。 所公开的方法可用于序列或检测靶核酸分子，例如存在于固定或不溶性样品中的核酸分子。