公开(公告)号：US20220106640A1

公开(公告)日：2022-04-07

申请号：US17416982

申请日：2019-12-02

Applicant: HTG Molecular Diagnostics, Inc.

Inventor： Debrah Thompson ,  Matthew Rounseville

IPC: C12Q1/6874 ,  C12Q1/6853

Abstract: The present disclosure provides methods for sequencing nucleic acid targets (e.g., both DNA and RNA co-amplified in a sample mixture, for example by using a surrogate for the RNA). Such methods can be used to determine if one or more nucleic acid targets are present in a sample.

公开(公告)号：US12104206B2

公开(公告)日：2024-10-01

申请号：US16070678

申请日：2017-02-10

Applicant: HTG Molecular Diagnostics, Inc.

Inventor： Debrah Thompson ,  Matthew Rounseville

IPC: C12Q1/6806 ,  C12Q1/6869

CPC classification number: C12Q1/6869 ,  C12Q1/6806 ,  C12Q1/6806 ,  C12Q2521/325 ,  C12Q2521/501 ,  C12Q2521/531 ,  C12Q2525/155 ,  C12Q2525/161 ,  C12Q2525/191 ,  C12Q2525/207 ,  C12Q2533/107 ,  C12Q2535/122 ,  C12Q2537/143 ,  C12Q2537/162 ,  C12Q2563/131 ,  C12Q2563/149 ,  C12Q1/6869 ,  C12Q2521/325 ,  C12Q2521/501 ,  C12Q2521/531 ,  C12Q2525/155 ,  C12Q2525/161 ,  C12Q2525/191 ,  C12Q2525/207 ,  C12Q2533/107 ,  C12Q2535/122 ,  C12Q2537/143 ,  C12Q2537/162 ,  C12Q2563/131 ,  C12Q2563/149

Abstract: The present disclosure provides methods and kits for direct sequencing of nucleic acid targets. Such methods can be used to determine if one or more nucleic acid targets are present in a sample.

公开(公告)号：US20160019337A1

公开(公告)日：2016-01-21

申请号：US14772038

申请日：2014-03-03

Applicant: HTG MOLECULAR DIAGNOSTICS, INC.

Inventor： Christopher Roberts ,  Hui Wang ,  Zhenquiang Lu ,  Krishna Maddula ,  Sam Rua ,  Kevin Knapp ,  Byron Lawson ,  Debrah Thompson ,  Michael Hrubiak ,  Tyler Breedlove ,  Vijay Modur

IPC: G06F19/20 ,  C12Q1/68

CPC classification number: G16B25/00 ,  C12Q1/6886 ,  C12Q2600/112 ,  C12Q2600/158 ,  C12Q2600/16 ,  G01N33/57423

Abstract: This disclosure concerns the identification of biomarkers that are characteristic of squamous or non squamous (e.g., adenocarcinoma, large cell carcinoma, carcinoid tumor, sarcomatoid carcinoma) subtypes of non small cell lung cancer (NSCLC), clinically useful NSCLC classifiers, kits and arrays for distinguishing squamous and nonsquamous NSCLC subtypes, bioinformatic methods for determining clinically useful classifiers, and methods of use of each of the foregoing.

Abstract translation: 本公开涉及鉴定非小细胞肺癌（NSCLC）的鳞状或非鳞状体（例如，腺癌，大细胞癌，类癌瘤，肉瘤样癌）亚型，临床上有用的NSCLC分类器，试剂盒和阵列的特征性生物标志物 区分鳞状和非鳞状NSCLC亚型，用于确定临床有用的分类器的生物信息学方法，以及上述每一种的使用方法。

公开(公告)号：US20140235460A1

公开(公告)日：2014-08-21

申请号：US14266884

申请日：2014-05-01

Applicant: HTG Molecular Diagnostics, Inc.

Inventor： Debrah Thompson ,  Bruce E. Seligmann ,  Debra A. Gordon

IPC: C12Q1/68

CPC classification number: C12Q1/6853 ,  C12Q1/6869 ,  C12Q1/6874 ,  C12Q2561/108

Abstract: The present disclosure provides an improvement to quantitative Nuclease Protection Assay (qNPA) and quantitative Nuclease Protection Sequencing (qNPS) methods. The disclosed methods use nuclease protection probes (NPPs) that include 5′-end and/or 3-end flanking sequences, which provide a universal hybridization and/or amplification sequence. The disclosed methods can be used to sequence or detect target nucleic acid molecules, such as those present in fixed or insoluble samples.

Abstract translation: 本公开提供了定量核酸酶保护测定（qNPA）和定量核酸酶保护测序（qNPS）方法的改进。 所公开的方法使用包括5'端和/或3端侧翼序列的核酸酶保护探针（NPP），其提供通用杂交和/或扩增序列。 所公开的方法可用于序列或检测靶核酸分子，例如存在于固定或不溶性样品中的核酸分子。

公开(公告)号：US20240425914A1

公开(公告)日：2024-12-26

申请号：US18687476

申请日：2022-08-29

Applicant: HTG Molecular Diagnostics, Inc.

Inventor： Debrah Thompson

IPC: C12Q1/6869 ,  C12Q1/6804 ,  C12Q1/6876

Abstract: The present disclosure provides methods of using quantitative nuclease protection sequencing (qNPS) methods to evaluate epitranscriptomes. The disclosed methods capture nucleic acid molecules that are modified as a result of exposure to an agent, such as stress, a mutagen, a microbe (such as a pathogen), or therapeutic agent. The resulting modified nucleic acid molecules are then directly or indirectly sequenced using nuclease protection probes (NPPs) and qNPS, allowing for the detection of target and off-target modifications of an epitranscriptome.

公开(公告)号：US20190017112A1

公开(公告)日：2019-01-17

申请号：US16070678

申请日：2017-02-10

Applicant: HTG Molecular Diagnostics, Inc.

Inventor： Debrah Thompson ,  Matthew Rounseville

IPC: C12Q1/6869

Abstract: The present disclosure provides methods and kits for direct sequencing of nucleic acid targets. Such methods can be used to determine if one or more nucleic acid targets are present in a sample.