公开(公告)号：US20220098551A1

公开(公告)日：2022-03-31

申请号：US17547863

申请日：2021-12-10

Applicant: Hadasit Medical Research Services and development Ltd.

Inventor： Benjamin Eithan REUBINOFF ,  Orna SINGER

IPC: C12N5/079 ,  A61P27/02 ,  A61K9/00 ,  A61K35/30

Abstract: A method of generating retinal pigment epithelial (RPE) cells is disclosed. The method comprises: (a) culturing human pluripotent stem cells in a human feeder cell-conditioned medium to obtain a cultured population of human pluripotent stem cells; (b) culturing said cultured population of human pluripotent stem cells in a medium comprising a differentiating agent to obtain differentiating cells; and (c) culturing said differentiating cells in a medium comprising one or more members of the TGFβ superfamily.

公开(公告)号：US20180312805A1

公开(公告)日：2018-11-01

申请号：US15771046

申请日：2016-10-26

Applicant: CELL CURE NEUROSCIENCES LTD. ,  HADASIT MEDICAL RESEARCH SERVICES AND DEVELOPMENT LTD.

Inventor： Benjamin Eithan REUBINOFF ,  Orna SINGER ,  Osnat BOHANA-KASHTAN ,  Ofer WISER

IPC: C12N5/079 ,  C12N5/0735 ,  C12N5/00

CPC classification number: C12N5/0621 ,  C12N2501/15 ,  C12N2501/16 ,  C12N2506/02 ,  C12N2533/52

Abstract: A method of generating retinal pigment epithelium cells is disclosed. Cell populations comprising same and uses thereof are also disclosed.

公开(公告)号：US20180216064A1

公开(公告)日：2018-08-02

申请号：US15748541

申请日：2016-07-28

Applicant: Hadasit Medical Research Services and Development Ltd.

Inventor： Benjamin Eithan REUBINOFF ,  Orna SINGER

IPC: C12N5/079 ,  A61K35/30 ,  A61K9/00 ,  A61P27/02

CPC classification number: C12N5/0621 ,  A61K9/0051 ,  A61K35/30 ,  A61P27/02 ,  C12N2500/38 ,  C12N2501/115 ,  C12N2501/15 ,  C12N2502/13 ,  C12N2506/02 ,  C12N2533/52 ,  C12N2533/54

Abstract: A method of generating retinal pigment epithelial (RPE) cells is disclosed. The method comprises: (a) culturing human pluripotent stem cells in a human feeder cell-conditioned medium to obtain a cultured population of human pluripotent stem cells; (b) culturing said cultured population of human pluripotent stem cells in a medium comprising a differentiating agent to obtain differentiating cells; and (c) culturing said differentiating cells in a medium comprising one or more members of the TGFβ superfamily.