公开(公告)号：US20070275464A1

公开(公告)日：2007-11-29

申请号：US10591576

申请日：2005-03-02

申请人： Hanae Kaku ,  Naoto Shibuya ,  Eiichi Minami ,  Naoko Minami ,  Yoko Nishizawa ,  Koji Takio ,  Naoshi Dohmae

发明人： Hanae Kaku ,  Naoto Shibuya ,  Eiichi Minami ,  Naoko Minami ,  Yoko Nishizawa ,  Koji Takio ,  Naoshi Dohmae

IPC分类号： A01H5/00 ,  A01N43/04 ,  C07H21/04 ,  C07K14/00 ,  C12N15/00 ,  C12N15/87 ,  C12N5/00

CPC分类号： C07K14/415 ,  A01N65/40 ,  C07K16/16 ,  C12N15/8218

摘要： The present inventors isolated and purified elicitor-binding proteins in good yield by combining the development of a column that uses APEA derivatives, pre-columns to remove non-specifically adsorbing substances, and effective elution methods. Using the N-terminal and internal chain amino acid sequences of the obtained proteins, the present inventors successfully isolated cDNAs encoding the proteins of the present invention from a rice cDNA library. Moreover, when anti-Con A-CEBiP antibodies were purified and their effect on elicitor-responsive reactive oxygen production was examined, production of reactive oxygen was inhibited by a pretreatment with the antibodies, suggesting that the present proteins are receptor proteins involved in chitin oligosaccharide elicitor responses. Since these elicitors induce resistance to blast in rice, the proteins of the present invention can be applied to the development of novel disease control technologies.

摘要翻译： 本发明人通过结合使用APEA衍生物的柱的发展，预柱除去非特异性吸附物质和有效的洗脱方法，以良好的产率分离并纯化了诱导物结合蛋白。 使用所得蛋白质的N末端和内部链氨基酸序列，本发明人成功地从水稻cDNA文库中分离了编码本发明蛋白质的cDNA。 此外，当抗Con A-CEBiP抗体被纯化并且检测其对诱导因子反应性活性氧产生的作用时，通过用抗体预处理抑制活性氧的产生，表明本发明的蛋白质是参与几丁质寡糖的受体蛋白 诱发反应。 由于这些诱导剂诱导稻瘟病抗性，因此本发明的蛋白质可用于开发新的疾病控制技术。