公开(公告)号：US20050034189A1

公开(公告)日：2005-02-10

申请号：US10871083

申请日：2004-06-18

申请人： Eiichi Minami ,  Naoto Shibuya ,  Robert Day

发明人： Eiichi Minami ,  Naoto Shibuya ,  Robert Day

IPC分类号： C07K14/415 ,  C12N15/29 ,  C12N15/82 ,  A01H1/00 ,  A01H5/00 ,  C07H21/04 ,  C12N5/04

CPC分类号： C12N15/8279 ,  C07K14/415

摘要： Genes whose expression was induced in the early stages of elicitor treatment were investigated using a DNA microchip containing 1265 varieties of rice ESTs. A chitin oligomer (N-acetylchitooligosaccharide), an important component in the cell wall of rice blast fungus, was used as the elicitor. This resulted in the identification of six varieties of novel elicitor-responsive ESTs. Of these, the product of two genes (named CIGR1 gene and CIGR2 gene) possessed a motif characteristic of the GRAS family, which are thought to be transcription factors. Thus it was indicated for the first time that, in addition to gibberellin signal transduction regulatory factors, the GRAS family is also present in rice.

摘要翻译： 使用含有1265个品种的EST的DNA微芯片研究在诱导剂治疗的早期阶段诱导其表达的基因。 甲壳质寡聚物（N-乙酰壳寡糖）是稻瘟菌真菌细胞壁的重要成分，作为诱发剂。 这导致了六种新型诱发因子反应ESTs的鉴定。 其中，两个基因（命名为CIGR1基因和CIGR2基因）的产物具有GRAS家族的基序特征，被认为是转录因子。 因此，首次表明除了赤霉素信号转导调控因子外，GRAS家族也存在于水稻中。

公开(公告)号：US20050153288A1

公开(公告)日：2005-07-14

申请号：US10496530

申请日：2002-12-20

申请人： Eiichi Minami ,  Naoto Shibuya ,  Robert Day

发明人： Eiichi Minami ,  Naoto Shibuya ,  Robert Day

IPC分类号： A01H3/00 ,  C07K14/415 ,  C12Q1/68

CPC分类号： C07K14/415

摘要： To date, those skilled in the art have never considered that cultured plant cells respond to gibberellins. However, to comprehensively and conveniently identify and isolate gibberellin responsive genes, cultured cells, which are uniform and easy to handle, are appropriate. Considering the advantages of cultured cells, the present inventors developed methods for identifying and isolating gibberellin responsive genes using cultured cells. After pre-culturing cells in an auxin-free medium, they examined whether or not changes in gene expression could be detected. As a result, it was surprisingly revealed that changes in the expression of some genes could be detected after gibberellin treatment.

摘要翻译： 迄今为止，本领域技术人员从未认为培养的植物细胞对赤霉素有反应。 然而，为了全面，方便地鉴定和分离赤霉素应答基因，培养的细胞是均匀且易于处理的。 考虑到培养细胞的优点，本发明人开发了使用培养细胞鉴定和分离赤霉素应答基因的方法。 在无生长素培养基中预培养细胞后，检查基因表达的变化是否被检测到。 结果令人吃惊地发现，赤霉素治疗后可以检测到某些基因的表达变化。

公开(公告)号：US20120023616A1

公开(公告)日：2012-01-26

申请号：US13256904

申请日：2010-03-16

申请人： Marie Nishimura ,  Yoko Nishizawa ,  Takashi Fujikawa ,  Ichiro Mitsuhara ,  Eiichi Minami ,  Keietsu Abe ,  Takashi Tachiki ,  Shigekazu Yano

发明人： Marie Nishimura ,  Yoko Nishizawa ,  Takashi Fujikawa ,  Ichiro Mitsuhara ,  Eiichi Minami ,  Keietsu Abe ,  Takashi Tachiki ,  Shigekazu Yano

IPC分类号： A01H1/00 ,  A61K38/47 ,  C12N5/10 ,  A61P31/04 ,  A01H5/00 ,  A61K35/66 ,  A61P31/10 ,  A01H5/10 ,  A61K35/74 ,  C12N15/82

CPC分类号： C12N15/8282 ,  C12N9/2402 ,  C12Y302/01084

摘要： This invention provides a method for preventing or inhibiting infection with a plant-infecting microorganism and imparting resistivity to plants, a method for preparing plants having resistance to diseases caused by microorganisms such as plant pathogenic filamentous fungi, and a microbial pesticide formulation. The method for preventing or inhibiting infection of a host plant with plant-infecting microorganisms comprises degrading α-1,3-glucan on cell walls of the microorganisms by α-1,3-glucanase.

摘要翻译： 本发明提供一种预防或抑制植物感染微生物感染并赋予植物电阻率的方法，一种制备具有抗病性的植物的方法，所述微生物如植物病原性丝状真菌和微生物农药制剂具有抗性。 用植物感染微生物预防或抑制宿主植物感染的方法包括通过α-1,3-葡聚糖酶降解微生物细胞壁上的α-1,3-葡聚糖。

公开(公告)号：US20070275464A1

公开(公告)日：2007-11-29

申请号：US10591576

申请日：2005-03-02

申请人： Hanae Kaku ,  Naoto Shibuya ,  Eiichi Minami ,  Naoko Minami ,  Yoko Nishizawa ,  Koji Takio ,  Naoshi Dohmae

发明人： Hanae Kaku ,  Naoto Shibuya ,  Eiichi Minami ,  Naoko Minami ,  Yoko Nishizawa ,  Koji Takio ,  Naoshi Dohmae

IPC分类号： A01H5/00 ,  A01N43/04 ,  C07H21/04 ,  C07K14/00 ,  C12N15/00 ,  C12N15/87 ,  C12N5/00

CPC分类号： C07K14/415 ,  A01N65/40 ,  C07K16/16 ,  C12N15/8218

摘要： The present inventors isolated and purified elicitor-binding proteins in good yield by combining the development of a column that uses APEA derivatives, pre-columns to remove non-specifically adsorbing substances, and effective elution methods. Using the N-terminal and internal chain amino acid sequences of the obtained proteins, the present inventors successfully isolated cDNAs encoding the proteins of the present invention from a rice cDNA library. Moreover, when anti-Con A-CEBiP antibodies were purified and their effect on elicitor-responsive reactive oxygen production was examined, production of reactive oxygen was inhibited by a pretreatment with the antibodies, suggesting that the present proteins are receptor proteins involved in chitin oligosaccharide elicitor responses. Since these elicitors induce resistance to blast in rice, the proteins of the present invention can be applied to the development of novel disease control technologies.

摘要翻译： 本发明人通过结合使用APEA衍生物的柱的发展，预柱除去非特异性吸附物质和有效的洗脱方法，以良好的产率分离并纯化了诱导物结合蛋白。 使用所得蛋白质的N末端和内部链氨基酸序列，本发明人成功地从水稻cDNA文库中分离了编码本发明蛋白质的cDNA。 此外，当抗Con A-CEBiP抗体被纯化并且检测其对诱导因子反应性活性氧产生的作用时，通过用抗体预处理抑制活性氧的产生，表明本发明的蛋白质是参与几丁质寡糖的受体蛋白 诱发反应。 由于这些诱导剂诱导稻瘟病抗性，因此本发明的蛋白质可用于开发新的疾病控制技术。

公开(公告)号：US4949157A

公开(公告)日：1990-08-14

申请号：US253186

申请日：1988-10-04

申请人： Eiichi Minami

发明人： Eiichi Minami

IPC分类号： H01L21/82 ,  H01L21/822 ,  H01L23/528 ,  H01L27/04 ,  H01L27/118

CPC分类号： H01L27/11807 ,  H01L23/528 ,  H01L2924/0002 ,  H01L2924/13091

摘要： This invention relates to an LSI in which a gate electrode wiring in a logic cell which is constructed by combining a plurality of basic cells each of which consisting of a pair of p-type and n-type MOS transistors and a gate electrode wiring, has portion extending substantially to a marginal region which does not contain logic cells. Additionally wiring portions associated with the LSI are disposed in a layer different from the layer to which the extended portions of the gate electrode wiring belongs. The extended portions, in the marginal region, are connectible portions through through holes. Such a structural arrangement results in an increase in the gate utilization ratio and improves the reliability of an LSI without decreasing the freedom to lead the wiring between gates.

摘要翻译： 本发明涉及一种LSI，其中通过组合由一对p型和n型MOS晶体管组成的多个基本单元和栅电极布线构成的逻辑单元中的栅电极布线具有 部分基本上延伸到不包含逻辑单元的边缘区域。 此外，与LSI相关联的布线部分设置在与栅电极布线的延伸部分所属的层不同的层中。 在边缘区域中的延伸部分是通过通孔的可连接部分。 这种结构布置导致栅极利用率的增加并且提高了LSI的可靠性，而不会降低引导栅极之间的布线的自由度。