公开(公告)号：US08431336B2

公开(公告)日：2013-04-30

申请号：US10332142

申请日：2001-07-06

申请人： Helen Lee ,  Magda Anastassova Dineva ,  Hsiang Yun Hu

发明人： Helen Lee ,  Magda Anastassova Dineva ,  Hsiang Yun Hu

IPC分类号： C12Q1/68 ,  C07H21/02 ,  C07H21/04 ,  C12P19/34

CPC分类号： C12Q1/689 ,  C12Q1/6832 ,  C12Q1/6834 ,  C12Q2565/519 ,  C12Q2525/197 ,  C12Q2565/625 ,  C12Q2537/125

摘要： Use of dipsticks to test for the presence of target nucleic acid in a sample solution is described. The dipsticks comprise a contact end for contacting the sample solution and a capture zone, remote from the contact end, to which a capture probe is immobilized. The capture probe is capable of hybridising to the target nucleic acid. The sample solution is contacted with the contact end of the dipstick and travels by capillary action to the capture zone. If target nucleic acid is present in the sample solution it is captured and can be detected at the capture zone. The capture probe is immobilized to the capture zone by a spacer. Use of the spacer increases the stability of the interaction between the capture probe and the target nucleic acid and thus improves the sensitivity of target nucleic acid detection. Detection probes with spacers are also described.

摘要翻译： 描述了使用骰子来测试样品溶液中靶核酸的存在。 骰子包括用于接触样品溶液的接触端和远离接触端的捕获区，捕获探针被固定到捕获区。 捕获探针能够与靶核酸杂交。 样品溶液与量油尺的接触端接触，并通过毛细作用行进到捕获区。 如果靶核酸存在于样品溶液中，则其被捕获并且可以在捕获区域被检测。 捕获探针通过间隔物固定在捕获区上。 间隔物的使用增加了捕获探针和目标核酸之间相互作用的稳定性，从而提高了靶核酸检测的灵敏度。 还描述了具有间隔物的检测探针。

公开(公告)号：US20120094272A1

公开(公告)日：2012-04-19

申请号：US13165624

申请日：2011-06-21

申请人： Helen Lee ,  Ling Huang ,  Magda Anastassova Dineva ,  Hsiang Yun Hu

发明人： Helen Lee ,  Ling Huang ,  Magda Anastassova Dineva ,  Hsiang Yun Hu

IPC分类号： G01N33/53 ,  G01N33/577 ,  C12Q1/70 ,  C07K16/46 ,  C07K1/107

CPC分类号： G01N33/558 ,  Y10S435/81 ,  Y10S436/807 ,  Y10S977/70 ,  Y10S977/773 ,  Y10S977/788 ,  Y10S977/918

摘要： Dipstick tests for detecting analyte are described. In a preferred embodiment, a multiple biotinylated antibody capable of binding analyte is bound to an anti-biotin antibody labelled with colloidal gold and wicked up the dipstick with test solution thought to contain analyte. Complex formed between analyte, biotinylated anti-analyte antibody, and colloidal gold labelled anti-biotin antibody is captured at a capture zone of the dipstick. Presence of colloidal gold label at the capture zone indicates the presence of analyte in the test solution. The sensitivity of analyte detection using such methods is an order of magnitude higher than for comparable methods in which biotinylated anti-analyte antibody bound to analyte is wicked up the dipstick in a first step, and a colloidal gold labelled anti-biotin antibody is wicked up the dipstick in a separate step. Kits for performing the tests of the invention are also described.

摘要翻译： 描述了用于检测分析物的浸渍试验。 在优选的实施方案中，能够结合分析物的多重生物素化抗体与由胶体金标记的抗生物素抗体结合，并且将被认为含有分析物的测试溶液弄脏。 分析物，生物素化抗分析物抗体和胶体金标记的抗生物素抗体之间形成的复合物在量油尺的捕获区捕获。 在捕获区存在胶体金标记表示测试溶液中存在分析物。 使用这种方法的分析物检测的灵敏度比在第一步中将测得的与分析物结合的生物素化的抗分析物抗体被弄脏的可比较方法高一个数量级，并且胶体金标记的抗生物素抗体被恶化 量油尺在一个单独的步骤。 还描述了用于执行本发明的测试的套件。

公开(公告)号：US20080206853A1

公开(公告)日：2008-08-28

申请号：US12042762

申请日：2008-03-05

申请人： Helen Lee ,  Ling Huang ,  Magda Anastassova Dineva ,  Hsiang Yun Hu

发明人： Helen Lee ,  Ling Huang ,  Magda Anastassova Dineva ,  Hsiang Yun Hu

IPC分类号： C12M1/00 ,  B01J19/00

CPC分类号： G01N33/558 ,  Y10S435/81 ,  Y10S436/807 ,  Y10S977/70 ,  Y10S977/773 ,  Y10S977/788 ,  Y10S977/918

摘要： Dipstick tests for detecting analyte are described. In a preferred embodiment, a multiple biotinylated antibody capable of binding analyte is bound to an anti-biotin antibody labeled with colloidal gold and wicked up the dipstick with test solution thought to contain analyte. Complex formed between analyte, biotinylated anti-analyte antibody, and colloidal gold labeled anti-biotin antibody is captured at a capture zone of the dipstick. Presence of colloidal gold label at the capture zone indicates the presence of analyte in the test solution. The sensitivity of analyte detection using such methods is an order of magnitude higher than for comparable methods in which biotinylated anti-analyte antibody bound to analyte is wicked up the dipstick in a first step, and a colloidal gold labeled anti-biotin antibody is wicked up the dipstick in a separate step. Kits for performing the tests of the invention are also described.

摘要翻译： 描述了用于检测分析物的浸渍试验。 在优选的实施方案中，能够结合分析物的多重生物素化抗体与由胶体金标记的抗生物素抗体结合，并且将被认为含有分析物的测试溶液弄脏。 分析物，生物素化抗分析物抗体和胶体金标记的抗生物素抗体之间形成的复合物在量油尺的捕获区捕获。 在捕获区存在胶体金标记表示测试溶液中存在分析物。 使用这种方法的分析物检测的灵敏度比在第一步中将测得的与分析物结合的生物素化的抗分析物抗体被弄脏的可比较方法高一个数量级，并且胶体金标记的抗生物素抗体被恶化 量油尺在一个单独的步骤。 还描述了用于执行本发明的测试的套件。

公开(公告)号：US07192701B2

公开(公告)日：2007-03-20

申请号：US10332131

申请日：2001-07-06

申请人： Helen Lee ,  Hsiang Yun Hu ,  Magda Anastassova Dineva

发明人： Helen Lee ,  Hsiang Yun Hu ,  Magda Anastassova Dineva

IPC分类号： C12Q1/68 ,  C07H21/02 ,  C07H21/04

CPC分类号： C12Q1/6834 ,  C12Q1/689 ,  C12Q2565/625

摘要： Dipsticks for testing for the presence of a target nucleic acid in a sample solution are described the dipsticks comprise a universal capture probe immobilised at a capture zone of the dipstick. The universal capture probe is capable of hybridising to a hook capture probe which is hybridised to the target nucleic acid in the sample solution. A contact end of the dipstick is contacted with the sample solution to cause hook capture probe hybridised to the target nucleic acid to move by capillary action to the capture zone where the target nucleic acid can be detected. Use of the universal and hook capture probes allows dipsticks to be prepared which can be used to capture any target nucleic acid, thereby simplifying preparation of the dipsticks. Specificity of target nucleic acid capture is then achieved by use of an appropriate hook capture probe. Methods and kits are also described.

摘要翻译： 描述了用于测试样品溶液中目标核酸存在的浸渍条，浸渍条包括固定在量油尺的捕获区的通用捕获探针。 通用捕获探针能够与钩状捕获探针杂交，其与样品溶液中的靶核酸杂交。 将量油尺的接触端与样品溶液接触，使钩捕捉探针与目标核酸杂交，通过毛细管作用移动到可以检测靶核酸的捕获区。 使用通用和钩捕获探针可以制备可以用于捕获任何靶核酸的骰子，从而简化骰子的制备。 然后通过使用适当的钩捕获探针实现靶核酸捕获的特异性。 还描述了方法和试剂盒。

公开(公告)号：US09494584B2

公开(公告)日：2016-11-15

申请号：US13165624

申请日：2011-06-21

申请人： Helen Lee ,  Ling Huang ,  Magda Anastassova Dineva ,  Hsiang Yun Hu

发明人： Helen Lee ,  Ling Huang ,  Magda Anastassova Dineva ,  Hsiang Yun Hu

IPC分类号： G01N33/558

CPC分类号： G01N33/558 ,  Y10S435/81 ,  Y10S436/807 ,  Y10S977/70 ,  Y10S977/773 ,  Y10S977/788 ,  Y10S977/918

摘要： Dipstick tests for detecting analyte are described. In a preferred embodiment, a multiple biotinylated antibody capable of binding analyte is bound to an anti-biotin antibody labelled with colloidal gold and wicked up the dipstick with test solution thought to contain analyte. Complex formed between analyte, biotinylated anti-analyte antibody, and colloidal gold labelled anti-biotin antibody is captured at a capture zone of the dipstick. Presence of colloidal gold label at the capture zone indicates the presence of analyte in the test solution. The sensitivity of analyte detection using such methods is an order of magnitude higher than for comparable methods in which biotinylated anti-analyte antibody bound to analyte is wicked up the dipstick in a first step, and a colloidal gold labelled anti-biotin antibody is wicked up the dipstick in a separate step. Kits for performing the tests of the invention are also described.

摘要翻译： 描述了用于检测分析物的浸渍试验。 在优选的实施方案中，能够结合分析物的多重生物素化抗体与由胶体金标记的抗生物素抗体结合，并且将被认为含有分析物的测试溶液弄脏。 分析物，生物素化抗分析物抗体和胶体金标记的抗生物素抗体之间形成的复合物在量油尺的捕获区捕获。 在捕获区存在胶体金标记表示测试溶液中存在分析物。 使用这种方法的分析物检测的灵敏度比在第一步中将测得的与分析物结合的生物素化的抗分析物抗体被弄脏的可比较方法高一个数量级，并且胶体金标记的抗生物素抗体被恶化 量油尺在一个单独的步骤。 还描述了用于执行本发明的测试的套件。

公开(公告)号：US07972837B2

公开(公告)日：2011-07-05

申请号：US12042762

申请日：2008-03-05

申请人： Helen Lee ,  Ling Huang ,  Magda Anastassova Dineva ,  Hsiang Yun Hu

发明人： Helen Lee ,  Ling Huang ,  Magda Anastassova Dineva ,  Hsiang Yun Hu

IPC分类号： G01N33/53

CPC分类号： G01N33/558 ,  Y10S435/81 ,  Y10S436/807 ,  Y10S977/70 ,  Y10S977/773 ,  Y10S977/788 ,  Y10S977/918

摘要： Dipstick tests for detecting analyte are described. In a preferred embodiment, a multiple biotinylated antibody capable of binding analyte is bound to an anti-biotin antibody labeled with colloidal gold and wicked up the dipstick with test solution thought to contain analyte. Complex formed between analyte, biotinylated anti-analyte antibody, and colloidal gold labeled anti-biotin antibody is captured at a capture zone of the dipstick. Presence of colloidal gold label at the capture zone indicates the presence of analyte in the test solution. The sensitivity of analyte detection using such methods is an order of magnitude higher than for comparable methods in which biotinylated anti-analyte antibody bound to analyte is wicked up the dipstick in a first step, and a colloidal gold labeled anti-biotin antibody is wicked up the dipstick in a separate step. Kits for performing the tests of the invention are also described.

摘要翻译： 描述了用于检测分析物的浸渍试验。 在优选的实施方案中，能够结合分析物的多重生物素化抗体与由胶体金标记的抗生物素抗体结合，并且将被认为含有分析物的测试溶液弄脏。 分析物，生物素化抗分析物抗体和胶体金标记的抗生物素抗体之间形成的复合物在量油尺的捕获区捕获。 在捕获区存在胶体金标记表示测试溶液中存在分析物。 使用这种方法的分析物检测的灵敏度比在第一步中将测得的与分析物结合的生物素化的抗分析物抗体被弄脏的可比较方法高一个数量级，并且胶体金标记的抗生物素抗体被恶化 量油尺在一个单独的步骤。 还描述了用于执行本发明的测试的套件。

公开(公告)号：US07776617B2

公开(公告)日：2010-08-17

申请号：US10432036

申请日：2001-11-30

申请人： Helen Lee ,  Ling Huang ,  Magda Anastassova Dineva ,  Hsiang Yun Hu

发明人： Helen Lee ,  Ling Huang ,  Magda Anastassova Dineva ,  Hsiang Yun Hu

IPC分类号： C12Q1/00 ,  C12M1/34 ,  C12M3/00 ,  G01N33/53 ,  G01N21/00 ,  G01N21/75 ,  G01N21/76 ,  G01N21/77 ,  G01N33/566 ,  G01N33/543 ,  G01N33/551 ,  G01N33/544

CPC分类号： G01N33/558 ,  Y10S435/81 ,  Y10S436/807 ,  Y10S977/70 ,  Y10S977/773 ,  Y10S977/788 ,  Y10S977/918

摘要： Dipstick tests for detecting analyte are described. In a preferred embodiment, a multiple biotinylated antibody capable of binding analyte is bound to an anti-biotin antibody labelled with colloidal gold and wicked up the dipstick with test solution thought to contain analyte. Complex formed between analyte, biotinylated anti-analyte antibody, and colloidal gold labelled anti-biotin antibody is captured at a capture zone of the dipstick. Presence of colloidal gold label at the capture zone indicates the presence of analyte in the test solution. The sensitivity of analyte detection using such methods is an order of magnitude higher than for comparable methods in which biotinylated anti-analyte antibody bound to analyte is wicked up the dipstick in a first step, and a colloidal gold labelled anti-biotin antibody is wicked up the dipstick in a separate step. Kits for performing the tests of the invention are also described.

摘要翻译： 描述了用于检测分析物的浸渍试验。 在优选的实施方案中，能够结合分析物的多重生物素化抗体与由胶体金标记的抗生物素抗体结合，并且将被认为含有分析物的测试溶液弄脏。 分析物，生物素化抗分析物抗体和胶体金标记的抗生物素抗体之间形成的复合物在量油尺的捕获区捕获。 在捕获区存在胶体金标记表示测试溶液中存在分析物。 使用这种方法的分析物检测的灵敏度比在第一步中将测得的与分析物结合的生物素化的抗分析物抗体被弄脏的可比较方法高一个数量级，并且胶体金标记的抗生物素抗体被恶化 量油尺在一个单独的步骤。 还描述了用于执行本发明的测试的套件。

公开(公告)号：US20080160516A1

公开(公告)日：2008-07-03

申请号：US11686580

申请日：2007-03-15

申请人： Helen LEE ,  Hsiang Yun Hu ,  Magda Anastassova Dineva

发明人： Helen LEE ,  Hsiang Yun Hu ,  Magda Anastassova Dineva

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6834 ,  C12Q1/689 ,  C12Q2565/625

摘要： Dipsticks for testing for the presence of a target nucleic acid in a sample solution are described the dipsticks comprise a universal capture probe immobilised at a capture zone of the dipstick. The universal capture probe is capable of hybridising to a hook capture probe which is hybridised to the target nucleic acid in the sample solution. A contact end of the dipstick is contacted with the sample solution to cause hook capture probe hybridised to the target nucleic acid to move by capillary action to the capture zone where the target nucleic acid can be detected. Use of the universal and hook capture probes allows dipsticks to be prepared which can be used to capture any target nucleic acid, thereby simplifying preparation of the dipsticks. Specificity of target nucleic acid capture is then achieved by use of an appropriate hook capture probe. Methods and kits are also described.

摘要翻译： 描述了用于测试样品溶液中目标核酸存在的浸渍条，浸渍条包括固定在量油尺的捕获区的通用捕获探针。 通用捕获探针能够与钩状捕获探针杂交，其与样品溶液中的靶核酸杂交。 将量油尺的接触端与样品溶液接触，使钩捕捉探针与目标核酸杂交，通过毛细管作用移动到可以检测靶核酸的捕获区。 使用通用和钩捕获探针可以制备可以用于捕获任何靶核酸的骰子，从而简化骰子的制备。 然后通过使用适当的钩捕获探针实现靶核酸捕获的特异性。 还描述了方法和试剂盒。