公开(公告)号：US4997927A

公开(公告)日：1991-03-05

申请号：US770295

申请日：1985-08-27

申请人： Helmut Blocker ,  Ronald Frank ,  Gudrun Heisterberg-Moutsis ,  Gisela Kurth ,  Andreas Meyerhans

发明人： Helmut Blocker ,  Ronald Frank ,  Gudrun Heisterberg-Moutsis ,  Gisela Kurth ,  Andreas Meyerhans

IPC分类号： C07H1/06 ,  C07H21/00 ,  C07H21/04

CPC分类号： C07H21/00 ,  Y02P20/55

摘要： A process for the purfication of synthetic oligonucleotides is described which drastically simplifies the hitherto very time consuming purification process. The deprotection solution is adsorbed without being concentrated on a carrier having reversed phase properties and optionally ion exchanger properties, the oligonuleotides without lipophilic protecting group are removed, their protecting group is cleaved off from the adsorbed oligonucleotides, the adsorbed oligonucleotides are washed and eluted from the carrier and then purified in a manner known per se.

摘要翻译： 描述了合成寡核苷酸的纯化方法，其大大简化了迄今非常耗时的纯化过程。 将脱保护溶液吸附而不被浓缩在具有反相特性和任选离子交换性质的载体上，除去没有亲脂性保护基团的寡核苷酸，将其保护基从吸附的寡核苷酸上切下，将吸附的寡核苷酸从 载体，然后以本身已知的方式纯化。