-
公开(公告)号：US08916356B2
公开(公告)日：2014-12-23
申请号：US11919431
申请日：2006-04-26
申请人： Hendrik Coenraad Hemker , Suzette Beguin , Raed Al-Dieri , Robert Wagenvoord , Sebastiaan Nijhuis , Peter Giesen
发明人： Hendrik Coenraad Hemker , Suzette Beguin , Raed Al-Dieri , Robert Wagenvoord , Sebastiaan Nijhuis , Peter Giesen
IPC分类号： C12Q1/56
CPC分类号： C12Q1/56 , G01N2333/974
摘要： The invention relates to a method for in vitro determining thrombin activity in a sample wherein the sample is a blood sample and thrombin generation is measured by the steps of: —contacting a layer of said sample with a fluorogenic substrate of thrombin, wherein said layer has a thickness within a range of 0.05 to 5 mm and a surface within a range of 10 to 500 mm2; —allowing thrombin to generate in said sample; —measuring the fluorescence emitted from the surface of the layer, by the fluorescent group released from the fluorogenic substrate as a result of enzymatic action of generated thrombin on said fluorogenic substrate.
摘要翻译： 本发明涉及一种用于体外测定样品中凝血酶活性的方法，其中样品是血液样品，并且通过以下步骤测量凝血酶产生： - 用凝血酶的荧光底物接触所述样品层，其中所述层具有 厚度在0.05〜5mm的范围内，表面在10〜500mm 2的范围内; - 使凝血酶在所述样品中产生; - 通过荧光基质释放的荧光基团测定从该表面发射的荧光，这是由于在所述荧光底物上产生的凝血酶的酶作用的结果。
-
公开(公告)号：US20090311730A1
公开(公告)日：2009-12-17
申请号：US11919431
申请日：2006-04-26
申请人： Hendrik Coenraad Hemker , Suzette Beguin , Raed Al-Dieri , Robert Wagenvoord , Sebastiaan Nijhuis , Peter Giesen
发明人： Hendrik Coenraad Hemker , Suzette Beguin , Raed Al-Dieri , Robert Wagenvoord , Sebastiaan Nijhuis , Peter Giesen
IPC分类号： C12Q1/56
CPC分类号： C12Q1/56 , G01N2333/974
摘要： The invention relates to a method for in vitro determining thrombin activity in a sample wherein the sample is a blood sample and thrombin generation is measured by the steps of: —contacting a layer of said sample with a fluorogenic substrate of thrombin, wherein said layer has a thickness within a range of 0.05 to 5 mm and a surface within a range of 10 to 500 mm2; —allowing thrombin to generate in said sample; —measuring the fluorescence emitted from the surface of the layer, by the fluorescent group released from the fluorogenic substrate as a result of enzymatic action of generated thrombin on said fluorogenic substrate.
摘要翻译： 本发明涉及一种用于体外测定样品中凝血酶活性的方法，其中样品是血液样品，并且通过以下步骤测量凝血酶产生： - 用凝血酶的荧光底物接触所述样品层，其中所述层具有 厚度在0.05〜5mm的范围内，表面在10〜500mm 2的范围内; - 使凝血酶在所述样品中产生; - 通过荧光基质释放的荧光基团测定从该表面发射的荧光，这是由于在所述荧光底物上产生的凝血酶的酶作用的结果。
-
公开(公告)号：US08828730B2
公开(公告)日：2014-09-09
申请号：US13057898
申请日：2009-08-05
IPC分类号： G01N33/543
CPC分类号： G01N33/86
摘要： Disclosed is a method for measuring thrombin generation in a whole blood sample. The whole blood sample may be applied forthwith, without prior processing. The blood cells and blood plasma in the whole blood sample are separated by (lateral) flow migration. Also disclosed is an assembly of a sample support and a device dedicated to measure thrombin generation in a whole blood sample. Advantageously, the sample support comprises a separator medium allowing separation of whole blood into blood cells and blood plasma by means of (lateral) flow migration.
摘要翻译： 公开了一种测定全血样品中凝血酶产生的方法。 全血样品可以立即应用，无需事先处理。 全血样品中的血细胞和血浆被（侧向）流动迁移分离。 还公开了样品载体和专用于测量全血样品中的凝血酶产生的装置的组装。 有利的是，样品载体包括分离介质，其允许通过（侧向）流动迁移将全血分离成血细胞和血浆。
-
公开(公告)号：US20110195441A1
公开(公告)日：2011-08-11
申请号：US13057898
申请日：2009-08-05
CPC分类号： G01N33/86
摘要： Disclosed is a method for measuring thrombin generation in a whole blood sample. The whole blood sample may be applied forthwith, without prior processing. The blood cells and blood plasma in the whole blood sample are separated by (lateral) flow migration. Also disclosed is an assembly of a sample support and a device dedicated to measure thrombin generation in a whole blood sample. Advantageously, the sample support comprises a separator medium allowing separation of whole blood into blood cells and blood plasma by means of (lateral) flow migration.
摘要翻译： 公开了一种测定全血样品中凝血酶产生的方法。 全血样品可以立即应用，无需事先处理。 全血样品中的血细胞和血浆被（侧向）流动迁移分离。 还公开了样品载体和专用于测量全血样品中的凝血酶产生的装置的组装。 有利的是，样品载体包括分离介质，其允许通过（侧向）流动迁移将全血分离成血细胞和血浆。
-
-
-
搜索结果
4 条记录 (耗时 0.023 秒)
