公开(公告)号：US08916356B2

公开(公告)日：2014-12-23

申请号：US11919431

申请日：2006-04-26

申请人： Hendrik Coenraad Hemker ,  Suzette Beguin ,  Raed Al-Dieri ,  Robert Wagenvoord ,  Sebastiaan Nijhuis ,  Peter Giesen

发明人： Hendrik Coenraad Hemker ,  Suzette Beguin ,  Raed Al-Dieri ,  Robert Wagenvoord ,  Sebastiaan Nijhuis ,  Peter Giesen

IPC分类号： C12Q1/56

CPC分类号： C12Q1/56 ,  G01N2333/974

摘要： The invention relates to a method for in vitro determining thrombin activity in a sample wherein the sample is a blood sample and thrombin generation is measured by the steps of: —contacting a layer of said sample with a fluorogenic substrate of thrombin, wherein said layer has a thickness within a range of 0.05 to 5 mm and a surface within a range of 10 to 500 mm2; —allowing thrombin to generate in said sample; —measuring the fluorescence emitted from the surface of the layer, by the fluorescent group released from the fluorogenic substrate as a result of enzymatic action of generated thrombin on said fluorogenic substrate.

摘要翻译： 本发明涉及一种用于体外测定样品中凝血酶活性的方法，其中样品是血液样品，并且通过以下步骤测量凝血酶产生： - 用凝血酶的荧光底物接触所述样品层，其中所述层具有 厚度在0.05〜5mm的范围内，表面在10〜500mm 2的范围内; - 使凝血酶在所述样品中产生; - 通过荧光基质释放的荧光基团测定从该表面发射的荧光，这是由于在所述荧光底物上产生的凝血酶的酶作用的结果。

公开(公告)号：US20090311730A1

公开(公告)日：2009-12-17

申请号：US11919431

申请日：2006-04-26

申请人： Hendrik Coenraad Hemker ,  Suzette Beguin ,  Raed Al-Dieri ,  Robert Wagenvoord ,  Sebastiaan Nijhuis ,  Peter Giesen

发明人： Hendrik Coenraad Hemker ,  Suzette Beguin ,  Raed Al-Dieri ,  Robert Wagenvoord ,  Sebastiaan Nijhuis ,  Peter Giesen

IPC分类号： C12Q1/56

CPC分类号： C12Q1/56 ,  G01N2333/974

摘要： The invention relates to a method for in vitro determining thrombin activity in a sample wherein the sample is a blood sample and thrombin generation is measured by the steps of: —contacting a layer of said sample with a fluorogenic substrate of thrombin, wherein said layer has a thickness within a range of 0.05 to 5 mm and a surface within a range of 10 to 500 mm2; —allowing thrombin to generate in said sample; —measuring the fluorescence emitted from the surface of the layer, by the fluorescent group released from the fluorogenic substrate as a result of enzymatic action of generated thrombin on said fluorogenic substrate.

摘要翻译： 本发明涉及一种用于体外测定样品中凝血酶活性的方法，其中样品是血液样品，并且通过以下步骤测量凝血酶产生： - 用凝血酶的荧光底物接触所述样品层，其中所述层具有 厚度在0.05〜5mm的范围内，表面在10〜500mm 2的范围内; - 使凝血酶在所述样品中产生; - 通过荧光基质释放的荧光基团测定从该表面发射的荧光，这是由于在所述荧光底物上产生的凝血酶的酶作用的结果。

公开(公告)号：US20060051828A1

公开(公告)日：2006-03-09

申请号：US10514269

申请日：2003-05-01

申请人： Peter Giesen ,  Hendrik Hemker ,  Raed Al Dieri ,  Suzette Beguin ,  Robert Wagenvoord

发明人： Peter Giesen ,  Hendrik Hemker ,  Raed Al Dieri ,  Suzette Beguin ,  Robert Wagenvoord

IPC分类号： C12Q1/56 ,  G06F19/00

CPC分类号： G01N33/86 ,  C12Q1/56

摘要： A method is provided for determining in real time the course of thrombin activity in a sample of blood or plasma as it appears in and disappears from the simple which comprises adding a thrombin substrate to the sample that, per unit time, produces a detectable signal in a quantity that bears relation to the amount of thrombin present. Simultaneously, in a control sample of the same blood or plasma in which thrombin generation is not triggered, the activity of a standard preparation with invariable thrombin activity is measured. The exact molar amount of thrombin present at any moment is obtained by comparison of the activity measured in clotting blood and the simultaneously measured calibrator. The method is useful inter alia for diagnosing hyper- and hypo-coaguable states, either congenital, acquired or drug-induced in humans and animals. Also provided is a kit for use in this method.

摘要翻译： 提供了一种方法，用于实时测定血液或血浆样品中凝血酶活性的过程，因为其在简单中出现并消失，其包括向样品中加入凝血酶底物，每单位时间产生可检测的信号 与存在凝血酶量有关的数量。 同时，在没有引发凝血酶生成的相同血液或血浆的对照样品中，测定具有不变凝血酶活性的标准制剂的活性。 通过比较在凝血血液和同时测量的校准物中测量的活性，可以获得随时存在的凝血酶的确切摩尔量。 该方法尤其用于诊断在人和动物中先天性，获得性或药物诱导的超和不可凝结状态。 还提供了用于该方法的试剂盒。

公开(公告)号：US08802386B2

公开(公告)日：2014-08-12

申请号：US10514269

申请日：2003-05-01

申请人： Peter Giesen ,  Hendrik Hemker ,  Raed Al Dieri ,  Suzette L. Beguin ,  Robert Wagenvoord

发明人： Peter Giesen ,  Hendrik Hemker ,  Raed Al Dieri ,  Suzette L. Beguin ,  Robert Wagenvoord

IPC分类号： C12Q1/56

CPC分类号： G01N33/86 ,  C12Q1/56

摘要： A method is provided for determining in real time the course of thrombin activity in a sample of blood or plasma as it appears in and disappears from the simple which comprises adding a thrombin substrate to the sample that, per unit time, produces a detectable signal in a quantity that bears relation to the amount of thrombin present. Simultaneously, in a control sample of the same blood or plasma in which thrombin generation is not triggered, the activity of a standard preparation with invariable thrombin activity is measured. The exact molar amount of thrombin present at any moment is obtained by comparison of the activity measured in clotting blood and the simultaneously measured calibrator. The method is useful inter alia for diagnosing hyper- and hypo-coaguable states, either congenital, acquired or drug-induced in humans and animals. Also provided is a kit for use in this method.

摘要翻译： 提供了一种方法，用于实时测定血液或血浆样品中凝血酶活性的过程，因为它在简单中出现并消失，其中包括向样品中加入凝血酶底物，每单位时间产生可检测的信号 与存在凝血酶量有关的数量。 同时，在没有引发凝血酶生成的相同血液或血浆的对照样品中，测定具有不变凝血酶活性的标准制剂的活性。 通过比较在凝血血液和同时测量的校准物中测量的活性，可以获得随时存在的凝血酶的确切摩尔量。 该方法尤其用于诊断在人和动物中先天性，获得性或药物诱导的超和不可凝结状态。 还提供了用于该方法的试剂盒。

公开(公告)号：US06864957B2

公开(公告)日：2005-03-08

申请号：US10424978

申请日：2003-04-29

申请人： Jan Van Elp ,  Peter Giesen ,  Jacob Jan Van der Velde

发明人： Jan Van Elp ,  Peter Giesen ,  Jacob Jan Van der Velde

IPC分类号： G03F7/20 ,  H01L21/027 ,  H01L21/683 ,  H02N13/00 ,  G03B27/42

CPC分类号： G03F7/70708 ,  G03F7/707 ,  G03F7/7095 ,  H01L21/6831

摘要： A lithographic projection apparatus with a chuck in which a dielectric element of the electrostatic chuck has a specific resistivity of at least 1016 Ωcm so that once the potential difference between electrodes of the chuck is removed the force on the article to be clamped reduces below a predetermined minimum level quickly. The dielectric element also has a coefficient of thermal expansion of less than 0.02×10−6K−1. A method of manufacturing a chuck includes joining a first glass ceramic element with a second glass element with an electrode therebetween in which a current is passed through the second glass element.

摘要翻译： 具有卡盘的光刻投影装置，其中静电卡盘的电介质元件的电阻率至少为10 16Ωgm，使得一旦卡盘的电极之间的电位差被去除，待夹紧物品上的力就减小 低于预定的最低水平。 电介质元件的热膨胀系数也小于0.02×10 -6 K -1。 制造卡盘的方法包括：将第一玻璃陶瓷元件与第二玻璃元件接合，其中电流通过第二玻璃元件。