公开(公告)号：US09897606B2

公开(公告)日：2018-02-20

申请号：US15219976

申请日：2016-07-26

申请人： Her Majesty the Queen in Right of Canada as represented by the Minister of Health

发明人： Xuguang Li ,  Runtao He ,  Gary Van Domselaar

IPC分类号： A61K45/06 ,  G01N33/569 ,  C12N9/24

CPC分类号： G01N33/56983 ,  A61K39/12 ,  A61K39/145 ,  A61K2039/505 ,  A61K2039/55566 ,  A61K2039/6081 ,  C07K16/1018 ,  C07K16/40 ,  C07K2317/34 ,  C12N9/2402 ,  C12N2760/16134 ,  C12Y302/01018 ,  G01N33/573 ,  G01N2333/924 ,  G01N2333/9506

摘要： Two universally conserved sequences from influenza type A neuraminidases were identified by large scale sequence analysis then chemically modified and conjugated to carrier proteins to generate mono-specific and monoclonal antibodies. The two antibodies, one targeting the N-terminus of the type A neuraminidase and the other sequence close to enzymatic active site, were capable of binding to all 9 subtypes of neuraminidase while demonstrating remarkable specificity against the viral neuraminidase sequences since no cross-reactivity against allantoic proteins was observed. Quantitative analyses of NA using slot blot suggest that the antibodies can be used for NA antigen quantitation in vaccines. These represent the first time the antibody-based immunoassay can be used for NA quantitative determination.

公开(公告)号：US09427462B2

公开(公告)日：2016-08-30

申请号：US14556415

申请日：2014-12-01

申请人： Her Majesty the Queen in Right of Canada as Represented by the Minister of Health

发明人： Xuguang Li ,  Runtao He ,  Gary Van Domselaar

IPC分类号： C07K16/40 ,  A61K45/06 ,  A61K39/145 ,  C07K16/10 ,  G01N33/569 ,  G01N33/573 ,  C12N9/24 ,  A61K39/12 ,  A61K39/00

CPC分类号： G01N33/56983 ,  A61K39/12 ,  A61K39/145 ,  A61K2039/505 ,  A61K2039/55566 ,  A61K2039/6081 ,  C07K16/1018 ,  C07K16/40 ,  C07K2317/34 ,  C12N9/2402 ,  C12N2760/16134 ,  C12Y302/01018 ,  G01N33/573 ,  G01N2333/924 ,  G01N2333/9506

摘要： Two universally conserved sequences from influenza type A neuraminidases were identified by large scale sequence analysis then chemically modified and conjugated to carrier proteins to generate mono-specific and monoclonal antibodies. The two antibodies, one targeting the N-terminus of the type A neuraminidase and the other sequence close to enzymatic active site, were capable of binding to all 9 subtypes of neuraminidase while demonstrating remarkable specificity against the viral neuraminidase sequences since no cross-reactivity against allantoic proteins was observed. Quantitative analyses of NA using slot blot suggest that the antibodies can be used for NA antigen quantitation in vaccines. These represent the first time the antibody-based immunoassay can be used for NA quantitative determination.

公开(公告)号：US20170131277A1

公开(公告)日：2017-05-11

申请号：US15219976

申请日：2016-07-26

申请人： Her Majesty the Queen in Right of Canada as represented by the Minister of Health

发明人： Xuguang Li ,  Runtao He ,  Gary Van Domselaar

IPC分类号： G01N33/569 ,  C12N9/24

CPC分类号： G01N33/56983 ,  A61K39/12 ,  A61K39/145 ,  A61K2039/505 ,  A61K2039/55566 ,  A61K2039/6081 ,  C07K16/1018 ,  C07K16/40 ,  C07K2317/34 ,  C12N9/2402 ,  C12N2760/16134 ,  C12Y302/01018 ,  G01N33/573 ,  G01N2333/924 ,  G01N2333/9506

摘要： Two universally conserved sequences from influenza type A neuraminidases were identified by large scale sequence analysis then chemically modified and conjugated to carrier proteins to generate mono-specific and monoclonal antibodies. The two antibodies, one targeting the N-terminus of the type A neuraminidase and the other sequence close to enzymatic active site, were capable of binding to all 9 subtypes of neuraminidase while demonstrating remarkable specificity against the viral neuraminidase sequences since no cross-reactivity against allantoic proteins was observed. Quantitative analyses of NA using slot blot suggest that the antibodies can be used for NA antigen quantitation in vaccines. These represent the first time the antibody-based immunoassay can be used for NA quantitative determination.

公开(公告)号：US20150147784A1

公开(公告)日：2015-05-28

申请号：US14556415

申请日：2014-12-01

申请人： Her Majesty the Queen in Right of Canada as Represented by the Minister of Health

发明人： Xuguang Li ,  Runtao He ,  Gary Van Domselaar

IPC分类号： C07K16/40

CPC分类号： G01N33/56983 ,  A61K39/12 ,  A61K39/145 ,  A61K2039/505 ,  A61K2039/55566 ,  A61K2039/6081 ,  C07K16/1018 ,  C07K16/40 ,  C07K2317/34 ,  C12N9/2402 ,  C12N2760/16134 ,  C12Y302/01018 ,  G01N33/573 ,  G01N2333/924 ,  G01N2333/9506

摘要： Two universally conserved sequences from influenza type A neuraminidases were identified by large scale sequence analysis then chemically modified and conjugated to carrier proteins to generate mono-specific and monoclonal antibodies. The two antibodies, one targeting the N-terminus of the type A neuraminidase and the other sequence close to enzymatic active site, were capable of binding to all 9 subtypes of neuraminidase while demonstrating remarkable specificity against the viral neuraminidase sequences since no cross-reactivity against allantoic proteins was observed. Quantitative analyses of NA using slot blot suggest that the antibodies can be used for NA antigen quantitation in vaccines. These represent the first time the antibody-based immunoassay can be used for NA quantitative determination.

摘要翻译： 通过大规模序列分析鉴定了来自甲型流感A型神经氨酸酶的两种普遍保守的序列，然后化学修饰并与载体蛋白缀合以产生单特异性和单克隆抗体。 靶向A型神经氨酸酶的N末端的两种抗体和接近酶活性位点的其他序列能够结合神经氨酸酶的所有9种亚型，同时显示出对病毒神经氨酸酶序列的显着特异性，因为没有交叉反应 观察尿囊蛋白。 使用狭缝印迹的NA的定量分析表明抗体可用于疫苗中的NA抗原定量。 这些代表了首次基于抗体的免疫测定可用于NA定量测定。