利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

11 条记录 (耗时 0.011 秒)

    VIRAL PROTEASE
    4.
    发明申请
    VIRAL PROTEASE 审中-公开
    病毒保护

    公开(公告)号：US20070160981A1

    公开(公告)日：2007-07-12

    申请号：US11551789

    申请日：2006-10-23

    申请人： Xin Chen ,  Yu San Han ,  Tsu-An Hsu

    发明人： Xin Chen ,  Yu San Han ,  Tsu-An Hsu

    IPC分类号： C12Q1/70 ,  A61K38/16 ,  A61K38/10 ,  A61K31/522

    CPC分类号： C12N9/506 ,  A61K38/00 ,  C07K7/06 ,  C07K14/005 ,  C12N2770/20022 ,  C12Q1/37 ,  G01N2333/811 ,  G01N2333/9506 ,  G01N2500/02

    摘要： An isolated polypeptide comprising a sequence that includes X−5-X−4-X−3-X−2-X−1-X+1, wherein X−5 is Arg, Ala, Ser, or Glu; X−4 is Leu; X−3 is Lys, Ala, Gln, or Asn; X−2 is Gly or Ala; X−1 is Gly; and X+1 is Ala, Gly, Asn, Val, or Lys; and the polypeptide, upon contact with SARS-CoV PLP2, murine hepatitis virus PLP, or bovine coronavirus PLP, is cleaved between residues X−1 and X+1 Also disclosed are related nucleic acids, vectors, host cells, screening methods, and treatment methods.

    摘要翻译： 包含序列的分离的多肽，其包含X-1至N-4个-X 3 -X 3 -X 2 -X 2 / 其中X-5是Arg，Ala，Ser或Glu; X 4a是Leu; X 3 -3是Lys，Ala，Gln或Asn; X 2是Gly或Ala; X-1是Gly; 并且X 1 + 1是Ala，Gly，Asn，Val或Lys; 并且多肽在与SARS-CoV PLP2接触时，鼠肝炎病毒PLP或牛冠状病毒PLP在残基X 1 -X 1和X 1 +之间被切割。还公开了 相关核酸，载体，宿主细胞，筛选方法和治疗方法。

    Reagents and methods for detecting influenza virus proteins
    6.
    发明授权
    Reagents and methods for detecting influenza virus proteins 有权
    用于检测流感病毒蛋白质的试剂和方法

    公开(公告)号：US08926982B2

    公开(公告)日：2015-01-06

    申请号：US13322459

    申请日：2010-05-28

    申请人： Xuguang Li ,  Runtao He ,  Gary Van Domselaar

    发明人： Xuguang Li ,  Runtao He ,  Gary Van Domselaar

    IPC分类号： A61K39/145 ,  C07K16/10 ,  G01N33/569 ,  G01N33/573 ,  C12N9/24 ,  A61K39/00

    CPC分类号： G01N33/56983 ,  A61K39/12 ,  A61K39/145 ,  A61K2039/505 ,  A61K2039/55566 ,  A61K2039/6081 ,  C07K16/1018 ,  C07K16/40 ,  C07K2317/34 ,  C12N9/2402 ,  C12N2760/16134 ,  C12Y302/01018 ,  G01N33/573 ,  G01N2333/924 ,  G01N2333/9506

    摘要： Two universally conserved sequences from influenza type A neuraminidases were identified by large scale sequence analysis then chemically modified and conjugated to carrier proteins to generate mono-specific and monoclonal antibodies. The two antibodies, one targeting the N-terminus of the type A neuraminidase and the other sequence close to enzymatic active site, were capable of binding to all 9 subtypes of neuraminidase while demonstrating remarkable specificity against the viral neuraminidase sequences since no cross-reactivity against allantoic proteins was observed. Quantitative analyses of NA using slot blot suggest that the antibodies can be used for NA antigen quantitation in vaccines. These represent the first time the antibody-based immunoassay can be used for NA quantitative determination.

    摘要翻译： 通过大规模序列分析鉴定了来自甲型流感A型神经氨酸酶的两种普遍保守的序列，然后化学修饰并与载体蛋白缀合以产生单特异性和单克隆抗体。 靶向A型神经氨酸酶的N末端的两种抗体和接近酶活性位点的其他序列能够结合所有9种亚型的神经氨酸酶，同时显示出对病毒神经氨酸酶序列的显着特异性，因为没有交叉反应 观察尿囊蛋白。 使用狭缝印迹的NA的定量分析表明抗体可用于疫苗中的NA抗原定量。 这些代表了首次基于抗体的免疫测定可用于NA定量测定。

    Reagents and Methods for Detecting Influenza Virus Proteins
    9.
    发明申请
    Reagents and Methods for Detecting Influenza Virus Proteins 审中-公开
    用于检测流感病毒蛋白质的试剂和方法

    公开(公告)号：US20150147784A1

    公开(公告)日：2015-05-28

    申请号：US14556415

    申请日：2014-12-01

    申请人： Her Majesty the Queen in Right of Canada as Represented by the Minister of Health

    发明人： Xuguang Li ,  Runtao He ,  Gary Van Domselaar

    IPC分类号： C07K16/40

    CPC分类号： G01N33/56983 ,  A61K39/12 ,  A61K39/145 ,  A61K2039/505 ,  A61K2039/55566 ,  A61K2039/6081 ,  C07K16/1018 ,  C07K16/40 ,  C07K2317/34 ,  C12N9/2402 ,  C12N2760/16134 ,  C12Y302/01018 ,  G01N33/573 ,  G01N2333/924 ,  G01N2333/9506

    摘要： Two universally conserved sequences from influenza type A neuraminidases were identified by large scale sequence analysis then chemically modified and conjugated to carrier proteins to generate mono-specific and monoclonal antibodies. The two antibodies, one targeting the N-terminus of the type A neuraminidase and the other sequence close to enzymatic active site, were capable of binding to all 9 subtypes of neuraminidase while demonstrating remarkable specificity against the viral neuraminidase sequences since no cross-reactivity against allantoic proteins was observed. Quantitative analyses of NA using slot blot suggest that the antibodies can be used for NA antigen quantitation in vaccines. These represent the first time the antibody-based immunoassay can be used for NA quantitative determination.

    摘要翻译： 通过大规模序列分析鉴定了来自甲型流感A型神经氨酸酶的两种普遍保守的序列，然后化学修饰并与载体蛋白缀合以产生单特异性和单克隆抗体。 靶向A型神经氨酸酶的N末端的两种抗体和接近酶活性位点的其他序列能够结合神经氨酸酶的所有9种亚型，同时显示出对病毒神经氨酸酶序列的显着特异性，因为没有交叉反应 观察尿囊蛋白。 使用狭缝印迹的NA的定量分析表明抗体可用于疫苗中的NA抗原定量。 这些代表了首次基于抗体的免疫测定可用于NA定量测定。