公开(公告)号：US6140118A

公开(公告)日：2000-10-31

申请号：US372702

申请日：1999-08-11

申请人： Herng Tsai ,  Henry D. Hunt ,  Larry D. Bacon ,  Bernard C. Wentworth ,  Alice L. Wentworth

发明人： Herng Tsai ,  Henry D. Hunt ,  Larry D. Bacon ,  Bernard C. Wentworth ,  Alice L. Wentworth

IPC分类号： A01K67/027 ,  C12N5/074 ,  C12R1/91 ,  C12N5/00

CPC分类号： C12N5/0611

摘要： Disclosed herein are cultures of undifferentiated avian primordial germ cells/blastodermal cells. The cultures are capable of maintaining their undifferentiated characteristic and also their capability of expressing the EMA-1 epitope for extended periods when cultured in the presence of an isolate from avian navel (e.g. turkey navel extract). Methods of culturing such cultures using avian navel extracts, and culture media containing avian navel extract are also disclosed. Recombinant birds derived from these cultures are also disclosed. These cultures can be frozen for long-term preservation of germ line genomes.

摘要翻译： 本文公开了未分化的禽类原始生殖细胞/胚细胞细胞的培养物。 培养物能够在禽类肚脐（例如火鸡肚脐萃取物）的分离物存在下培养时保持其未分化特征以及长时间表达EMA-1表位的能力。 还公开了使用禽肚提取物培养这些培养物的方法，以及含有禽肚肚提取物的培养基。 还公开了衍生自这些培养物的重组鸟。 这些培养物可以冷冻以长期保存种系基因组。