Production of antisera specific to major histocompatibility complex
molecules in chickens
    2.
    发明授权
    Production of antisera specific to major histocompatibility complex molecules in chickens 失效
    生产对鸡主要组织相容性复合分子特异的抗血清

    公开(公告)号:US6075125A

    公开(公告)日:2000-06-13

    申请号:US890719

    申请日:1997-07-09

    IPC分类号: C07K16/28 C12N15/63 C12N15/85

    CPC分类号: C07K16/2833 C07K2317/34

    摘要: Host cells transfected with a recombinant DNA molecule which includes a DNA sequence inserted therein encoding a heterologous chicken BFIV MHC class I protein are disclosed. The transfected cells express the heterologous BFIV protein and may be used as an immunogen to produce chicken MHC class I (BFIV) specific antisera. The antisera so produced may then be used to determine the BF haplotype of any chicken.

    摘要翻译: 公开了用包含编码异源鸡BFIV MHC I类蛋白质的插入DNA序列的重组DNA分子转染的宿主细胞。 转染的细胞表达异源BFIV蛋白,可用作免疫原产生鸡MHC I类(BFIV)特异性抗血清。 如此产生的抗血清可用于确定任何鸡的BF单体型。

    Avian blastodermal cell lines
    3.
    发明授权
    Avian blastodermal cell lines 失效
    禽类胚胎细胞系

    公开(公告)号:US6140118A

    公开(公告)日:2000-10-31

    申请号:US372702

    申请日:1999-08-11

    CPC分类号: C12N5/0611

    摘要: Disclosed herein are cultures of undifferentiated avian primordial germ cells/blastodermal cells. The cultures are capable of maintaining their undifferentiated characteristic and also their capability of expressing the EMA-1 epitope for extended periods when cultured in the presence of an isolate from avian navel (e.g. turkey navel extract). Methods of culturing such cultures using avian navel extracts, and culture media containing avian navel extract are also disclosed. Recombinant birds derived from these cultures are also disclosed. These cultures can be frozen for long-term preservation of germ line genomes.

    摘要翻译: 本文公开了未分化的禽类原始生殖细胞/胚细胞细胞的培养物。 培养物能够在禽类肚脐(例如火鸡肚脐萃取物)的分离物存在下培养时保持其未分化特征以及长时间表达EMA-1表位的能力。 还公开了使用禽肚提取物培养这些培养物的方法,以及含有禽肚肚提取物的培养基。 还公开了衍生自这些培养物的重组鸟。 这些培养物可以冷冻以长期保存种系基因组。