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    Procedure for specific replacement of a copy of a gene present in the recipient genome by the integration of a gene different from that where the integration is made
    1.
    发明授权
    Procedure for specific replacement of a copy of a gene present in the recipient genome by the integration of a gene different from that where the integration is made 失效
    具体替换存在于受体基因组中的基因的拷贝通过整合不同于进行整合的基因的基因的程序

    公开(公告)号：US06638768B1

    公开(公告)日：2003-10-28

    申请号：US08466699

    申请日：1995-06-06

    Applicant: Hervé Le Mouellic ,  Philippe Brulet

    Inventor： Hervé Le Mouellic ,  Philippe Brulet

    IPC: C12N516

    CPC classification number: A01K67/0275 ,  A01K2217/05 ,  A01K2227/105 ,  A61K38/00 ,  A61K48/00 ,  C07K14/47 ,  C12N15/85 ,  C12N15/8509 ,  C12N15/907 ,  C12N2800/108 ,  C12N2800/30 ,  C12N2830/00 ,  C12N2830/85

    Abstract: The present invention relates to a process for targeted replacement of at least a part of an endogenous gene by at least a part of a foreign gene or targeted insertion of at least a part of a foreign gene at a targeted site in an endogenous gene in a cell by homologous recombination. This process includes (A) providing a vector which contains (1) at least a part of the foreign gene which is heterologous with respect to the endogenous gene, (2) a first flanking DNA sequence homologous to a first genomic sequence situated on one side of the part of the endogenous gene to be replaced or the targeted site; and (3) a second flanking DNA sequence homologous to a second genomic sequence situated on the other side of the part of the endogenous gene to be replaced or the targeted site, the foreign gene being located between the first and second flanking DNA sequences and is complementary to the part of the endogenous gene to be replaced; (B) transfecting a cell with the vector; (C) and selecting a transfected cell that contains the foreign gene at the targeted site or where the part of the endogenous gene is to be replaced. This invention also pertains to the vector, and the cell transfected by the vector.

    Abstract translation: 本发明涉及通过外源基因的至少一部分靶向置换至少一部分内源基因或至少部分外源基因靶向插入外源基因的内源基因的方法 细胞通过同源重组。 该方法包括（A）提供载体，该载体含有（1）相对于内源基因异源的外源基因的至少一部分，（2）与位于一侧的第一基因组序列同源的第一侧翼DNA序列 的待更换的内源性基因部分或靶位点; 和（3）与位于待替代的内源基因部分的另一侧的第二基因组序列同源的第二侧翼DNA序列或靶位点，外源基因位于第一和第二侧翼DNA序列之间，并且是 与待更换的内源基因的部分互补; （B）用载体转染细胞; （C），并且在靶位点选择含有外源基因的转染细胞，或者内源基因的一部分被置换的转染细胞。 本发明还涉及载体和载体转染的细胞。

    Procedure for specific replacement of a copy of a gene present in the recipient genome by the integration of a gene different from that where the integration is made
    3.
    发明授权
    Procedure for specific replacement of a copy of a gene present in the recipient genome by the integration of a gene different from that where the integration is made 失效
    具体替换存在于受体基因组中的基因的拷贝通过整合不同于进行整合的基因的基因的程序

    公开(公告)号：US06528313B1

    公开(公告)日：2003-03-04

    申请号：US08301037

    申请日：1994-09-06

    Applicant: Hervé Le Mouellic ,  Philippe Brulet

    Inventor： Hervé Le Mouellic ,  Philippe Brulet

    IPC: C12N1564

    CPC classification number: A01K67/0275 ,  A01K2217/05 ,  A01K2227/105 ,  A61K38/00 ,  A61K48/00 ,  C07K14/47 ,  C12N15/85 ,  C12N15/8509 ,  C12N15/907 ,  C12N2800/108 ,  C12N2800/30 ,  C12N2830/00 ,  C12N2830/85

    Abstract: The present invention relates to a process for targeted replacement of at least a part of an endogenous gene by at least a part of a foreign gene or targeted insertion of at least a part of a foreign gene at a targeted site in an endogenous gene in a cell by homologous recombination. This process includes (A) providing a vector which contains (1) at least a part of the foreign gene which is heterologous with respect to the endogenous gene; (2) a first flanking DNA sequence homologous to a first genomic sequence situated on one side of the part of the endogenous gene to be replaced or the targeted site; and (3) a second flanking DNA sequence homologous to a second genomic sequence situated on the other side of the part of the endogenous gene to be replaced or the targeted site, the foreign gene being located between the first and second flanking DNA sequences and is complementary to the part of the endogenous gene to be replaced; (B) transfecting a cell with the vector; (C) and selecting a transfected cell that contains the foreign gene at the targeted site or where the part of the endogenous gene is to be replaced.

    Abstract translation: 本发明涉及通过外源基因的至少一部分靶向置换至少一部分内源基因或至少部分外源基因靶向插入外源基因的内源基因的方法 细胞通过同源重组。 该方法包括（A）提供载体，其含有（1）外源基因的至少一部分相对于内源基因是异源的; （2）与位于待置换的内源基因的一部分的一侧上的第一基因组序列或靶位点同源的第一侧翼DNA序列; 和（3）与位于待替代的内源基因部分的另一侧的第二基因组序列同源的第二侧翼DNA序列或靶位点，外源基因位于第一和第二侧翼DNA序列之间，并且是 与待更换的内源基因的部分互补; （B）用载体转染细胞; （C），并且在靶位点选择含有外源基因的转染细胞，或者内源基因的一部分被置换的转染细胞。

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