公开(公告)号：US4550078A

公开(公告)日：1985-10-29

申请号：US495677

申请日：1983-05-18

申请人： Hideaki Yamada ,  Shogo Otsuji ,  Kimiyasu Isobe ,  Kuniyoshi Matsunaga ,  Tadahiko Inukai

发明人： Hideaki Yamada ,  Shogo Otsuji ,  Kimiyasu Isobe ,  Kuniyoshi Matsunaga ,  Tadahiko Inukai

IPC分类号： G01N33/52 ,  C12Q1/26 ,  G01N33/50

CPC分类号： C12Q1/26

摘要： A rapid and reliable method is proposed for the quantitative determination of the individual free and acetylated polyamines, i.e. spermine, spermidine, putrescine, acetylspermine, N.sup.1 - and N.sup.8 -acetylspermidines and acetylputrescine in the body fluid of a patient suffering cancer or other deseases to facilitate diagnosis and therapeutic treatment. The method comprises subjecting the aqueous fluid to several enzymatic oxidative decomposition reactions of the polyamines in sequence or in combination by use of the 4 enzymes, i.e. polyamine oxidase AT-1, polyamine oxidase PC-3, putrescine oxidase and acetylputrescine oxidase, and determining, preferably photometrically, the amount of hydrogen peroxide formed as a byproduct in each of the reaction steps followed by setting up a set of simultaneous equations to give the respective amounts of the individual polyamines as a function of the amounts of the hydrogen peroxide formed in the reaction steps and solving the simultaneous equations.

摘要翻译： 提出了一种快速可靠的方法，用于定量测定患有癌症或其他疾病的患者的体液中的各种游离和乙酰化多胺，即精胺，亚精胺，腐胺，乙酰精胺，N1-和N8-乙酰哌啶和乙酰吗啡 诊断和治疗。 该方法包括通过使用4种酶，即多胺氧化酶AT-1，多胺氧化酶PC-3，腐胺氧化酶和乙酰​​胸腺嘧啶氧化酶，依次或组合地对含水液体进行多种酶的氧化分解反应， 优选光度计，在每个反应步骤中作为副产物形成的过氧化氢的量随后建立一组联立方程，以得到作为在反应中形成的过氧化氢的量的函数的各个多胺的各自量 步骤和求解联立方程。

公开(公告)号：US4617263A

公开(公告)日：1986-10-14

申请号：US776162

申请日：1985-09-13

申请人： Hideaki Yamada ,  Shogo Otsuji ,  Kimiyasu Isobe ,  Kuniyoshi Matsunaga ,  Tadahiko Inukai

发明人： Hideaki Yamada ,  Shogo Otsuji ,  Kimiyasu Isobe ,  Kuniyoshi Matsunaga ,  Tadahiko Inukai

IPC分类号： G01N33/52 ,  C12Q1/26 ,  G01N33/50

CPC分类号： C12Q1/26

摘要： A rapid and reliable method is proposed for the quantitative determination of the individual free and acetylated polyamines, i.e. spermine, spermidine, putrescine, acetylspermine, N.sup.1 - and N.sup.8 -acetylspermidines and acetylputrescine in the body fluid of a patient suffering cancer or other diseases to facilitate diagnosis and therapeutic treatment. The method comprises subjecting the aqueous fluid to several enzymatic oxidative decomposition reactions of the polyamines in sequence or in combination by use of the 4 enzymes, i.e. polyamine oxidase AT-1, polyamine oxidase PC-3, putrescine oxidase and acetylputrescine oxidase, and determining, preferably photometrically, the amount of hydrogen peroxide formed as a byproduct in each of the reaction steps followed by setting up a set of simultaneous equations to give the respective amounts of the individual polyamines as a function of the amounts of the hydrogen peroxide formed in the reaction steps and solving the simultaneous equations.

摘要翻译： 提出了一种快速可靠的方法，用于定量测定患有癌症或其他疾病的患者的体液中的个体游离和乙酰化多胺，即精胺，亚精胺，腐胺，乙酰精胺，N1-和N8-乙酰精氨酸和乙酰胞嘧啶 诊断和治疗。 该方法包括通过使用4种酶，即多胺氧化酶AT-1，多胺氧化酶PC-3，腐胺氧化酶和乙酰​​胸腺嘧啶氧化酶，依次或组合地对含水液体进行多种酶的氧化分解反应， 优选光度计，在每个反应步骤中作为副产物形成的过氧化氢的量随后建立一组联立方程，以得到作为在反应中形成的过氧化氢的量的函数的各个多胺的各自量 步骤和求解联立方程。

公开(公告)号：US4568545A

公开(公告)日：1986-02-04

申请号：US508163

申请日：1983-06-27

申请人： Hisashi Mihara ,  Hiroyuki Sumi ,  Akira Matsuura ,  Tadahiko Inukai

发明人： Hisashi Mihara ,  Hiroyuki Sumi ,  Akira Matsuura ,  Tadahiko Inukai

IPC分类号： A61K38/00 ,  C12N9/64 ,  A61K37/54 ,  C12N9/48

CPC分类号： C12N9/6402 ,  A61K38/00 ,  Y10S514/822

摘要： The invention discloses that the tissues of earthworms contain fibrinolytically or thrombolytically active ingredients which can be extracted and purified by a suitable sequence of extraction and purification procedures into the individual active ingredients including six novel proteases named F-O-HM-45, F-I-1-HM-54, F-I-2-HM-15, F-II-HM-64, F-III-1-HM-27 and F-III-2-HM-89. The chromatographic fractionation of the earthworm extract with an aqueous extractant gives five active fractions, the first four of which contain each one of the first mentioned four proteases and the last of which contains the last mentioned two proteases. The disclosure includes description of the suitable purification methods for the proteases as well as the physico-chemical identification data thereof. Various thrombolytic medicament forms prepared with the novel proteases as the effective ingredient are described together with the results of the clinical tests carried out by the oral administration of the novel proteases.

摘要翻译： 本发明公开了蚯蚓的组织含有纤维蛋白溶解或血栓溶解活性成分，其可以通过适当的提取和纯化程序提取和纯化到各个活性成分中，包括六种新的命名为FO-HM-45，FI-1-HM -54，FI-2-HM-15，F-II-HM-64，F-III-1-HM-27和F-III-2-HM-89。 用含水萃取剂的蚯蚓提取物的色谱分离提供了五种活性级分，其中前四种含有前述四种蛋白酶中的每一种，最后含有最后提到的两种蛋白酶。 本公开包括对蛋白酶的合适的纯化方法的描述以及其物理化学鉴定数据。 用新型蛋白酶作为有效成分制备的各种血栓溶解药物形式与通过口服施用新型蛋白酶进行的临床试验的结果一并描述。

公开(公告)号：US6083931A

公开(公告)日：2000-07-04

申请号：US930680

申请日：1997-12-09

申请人： Takaaki Hasegawa ,  Kenichi Miyamoto ,  Mitsuo Kawase ,  Yasuko Yoshida ,  Tadahiko Inukai

发明人： Takaaki Hasegawa ,  Kenichi Miyamoto ,  Mitsuo Kawase ,  Yasuko Yoshida ,  Tadahiko Inukai

IPC分类号： A61K31/726 ,  A61P35/00 ,  C07H7/027 ,  A61K31/715

CPC分类号： A61K31/7012 ,  A61K31/726 ,  C07H7/027

摘要： The present invention is aimed at providing an anticancer agent which can inhibit the metastasis of cancer cells when used alone. In addition, the present invention is also aimed at providing an anticancer agent which can inhibit the metastasis of cancer cells while causing a small side effect and very small toxicity even for an extended time of administration. The cancer metastasis-inhibiting anticancer agent according to the present invention is characterized by containing sialic acid, its salt, a polymer of sialic acid or a salt of the polymer as an effective ingredient.

摘要翻译： PCT No.PCT / JP96 / 00268 Sec。 371日期1997年12月9日第 102（e）日期1997年12月9日PCT提交1996年2月8日PCT公布。 公开号WO97 / 28807 日期：1997年8月14日本发明旨在提供一种当单独使用时能抑制癌细胞转移的抗癌剂。 另外，本发明的目的还在于提供一种能够抑制癌细胞的转移，同时引起副作用小，毒性甚至延长施用时的毒性也非常小的抗癌​​剂。 根据本发明的癌转移抑制抗癌剂的特征在于含有唾液酸，其盐，唾液酸的聚合物或聚合物的盐作为有效成分。

公开(公告)号：US20120128861A1

公开(公告)日：2012-05-24

申请号：US13384556

申请日：2010-07-22

申请人： Shinichi Kitamura ,  Makoto Nakaya ,  Akiko Kubo ,  Tadahiko Inukai

发明人： Shinichi Kitamura ,  Makoto Nakaya ,  Akiko Kubo ,  Tadahiko Inukai

IPC分类号： A23L1/182

CPC分类号： A23L7/196 ,  A23L7/10

摘要： Provided are rice grains where various water-soluble high molecular substances are retained without applying pressure to the rice grains. Grains of mutant rice (wx/ae rice), which is deficient in both amylopectin branching enzyme (BEIIb) and amylose synthetase I (GBSSI), are immersed in an aqueous solution of a water-soluble high molecular substance such as digestion resistant dextrin, arabinogalactan, or polyphenol under ordinary pressure at room temperature and are then dried at 60° C. or less, preferably about 50° C., thereby producing rice grains impregnated with and retaining the high molecular substance.

摘要翻译： 提供在不向米粒施加压力的情况下保留各种水溶性高分子物质的稻谷。 在支链淀粉分支酶（BEIIb）和直链淀粉合成酶I（GBSSI）中缺乏的突变体水稻（wx / ae rice）的谷粒浸渍在水溶性高分子物质如消化糊精， 阿拉伯半乳聚糖或多酚，然后在室温下在常压下干燥，然后在60℃或更低，优选约50℃下干燥，从而产生浸渍并保留高分子物质的米粒。

公开(公告)号：US06395717B1

公开(公告)日：2002-05-28

申请号：US08776032

申请日：1997-01-23

申请人： Michio Ohta ,  Takaaki Hasegawa ,  Masayuki Nadai ,  Yasuko Yoshida ,  Mitsuo Kawase ,  Tadahiko Inukai

发明人： Michio Ohta ,  Takaaki Hasegawa ,  Masayuki Nadai ,  Yasuko Yoshida ,  Mitsuo Kawase ,  Tadahiko Inukai

IPC分类号： A61K31726

CPC分类号： A61K31/726 ,  A61K31/7012 ,  A61K31/715 ,  Y10S514/921

摘要： Therapeutic drugs for endotoxin blood symptom and multi-organ failure induced by it is provided, which therapeutic during are composed of sialic acid its salt, polymers of sialic acid or a salt of the polymer as effective components and have high therapeutic effects for shock death and organ failure induced by endotoxin as well as high safety, and are effective for treatment of endotoxin-shock, and organ failure.

摘要翻译： 提供了由其引起的内毒素血症和多器官功能衰竭的治疗药物，其由唾液酸盐，唾液酸聚合物或聚合物的盐作为有效成分组成，对休克死亡具有很高的治疗效果， 内毒素引起的器官功能衰竭以及安全性高，对内毒素休克和器官功能衰竭有效。