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1.发明授权
公开(公告)号：US4617263A
公开(公告)日：1986-10-14
申请号：US776162
申请日：1985-09-13
CPC分类号： C12Q1/26
摘要： A rapid and reliable method is proposed for the quantitative determination of the individual free and acetylated polyamines, i.e. spermine, spermidine, putrescine, acetylspermine, N.sup.1 - and N.sup.8 -acetylspermidines and acetylputrescine in the body fluid of a patient suffering cancer or other diseases to facilitate diagnosis and therapeutic treatment. The method comprises subjecting the aqueous fluid to several enzymatic oxidative decomposition reactions of the polyamines in sequence or in combination by use of the 4 enzymes, i.e. polyamine oxidase AT-1, polyamine oxidase PC-3, putrescine oxidase and acetylputrescine oxidase, and determining, preferably photometrically, the amount of hydrogen peroxide formed as a byproduct in each of the reaction steps followed by setting up a set of simultaneous equations to give the respective amounts of the individual polyamines as a function of the amounts of the hydrogen peroxide formed in the reaction steps and solving the simultaneous equations.
摘要翻译： 提出了一种快速可靠的方法，用于定量测定患有癌症或其他疾病的患者的体液中的个体游离和乙酰化多胺，即精胺，亚精胺，腐胺，乙酰精胺，N1-和N8-乙酰精氨酸和乙酰胞嘧啶 诊断和治疗。 该方法包括通过使用4种酶，即多胺氧化酶AT-1，多胺氧化酶PC-3，腐胺氧化酶和乙酰胸腺嘧啶氧化酶，依次或组合地对含水液体进行多种酶的氧化分解反应， 优选光度计，在每个反应步骤中作为副产物形成的过氧化氢的量随后建立一组联立方程，以得到作为在反应中形成的过氧化氢的量的函数的各个多胺的各自量 步骤和求解联立方程。
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2.发明授权Method for the quantitative determination of individual polyamine components in a mixture 失效用于定量测定混合物中单个多胺组分的方法
公开(公告)号：US4550078A
公开(公告)日：1985-10-29
申请号：US495677
申请日：1983-05-18
CPC分类号： C12Q1/26
摘要： A rapid and reliable method is proposed for the quantitative determination of the individual free and acetylated polyamines, i.e. spermine, spermidine, putrescine, acetylspermine, N.sup.1 - and N.sup.8 -acetylspermidines and acetylputrescine in the body fluid of a patient suffering cancer or other deseases to facilitate diagnosis and therapeutic treatment. The method comprises subjecting the aqueous fluid to several enzymatic oxidative decomposition reactions of the polyamines in sequence or in combination by use of the 4 enzymes, i.e. polyamine oxidase AT-1, polyamine oxidase PC-3, putrescine oxidase and acetylputrescine oxidase, and determining, preferably photometrically, the amount of hydrogen peroxide formed as a byproduct in each of the reaction steps followed by setting up a set of simultaneous equations to give the respective amounts of the individual polyamines as a function of the amounts of the hydrogen peroxide formed in the reaction steps and solving the simultaneous equations.
摘要翻译： 提出了一种快速可靠的方法，用于定量测定患有癌症或其他疾病的患者的体液中的各种游离和乙酰化多胺，即精胺，亚精胺，腐胺，乙酰精胺，N1-和N8-乙酰哌啶和乙酰吗啡 诊断和治疗。 该方法包括通过使用4种酶，即多胺氧化酶AT-1，多胺氧化酶PC-3，腐胺氧化酶和乙酰胸腺嘧啶氧化酶，依次或组合地对含水液体进行多种酶的氧化分解反应， 优选光度计，在每个反应步骤中作为副产物形成的过氧化氢的量随后建立一组联立方程，以得到作为在反应中形成的过氧化氢的量的函数的各个多胺的各自量 步骤和求解联立方程。
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公开(公告)号：US4999289A
公开(公告)日：1991-03-12
申请号：US34452
申请日：1987-04-06
CPC分类号： C12N9/20 , C12Q1/44 , Y10S435/933
摘要： Lipase isolated from Penicillium characterized by the following properties:(1) Efficiently hydrolyzes triglycerides of fatty acids having 4-18 carbon atoms;(2) Hydrolysis of such triglycerides with such lipase produces at least 5 moles of glycerol per 100 moles of fatty acid;(3) Optimal pH range for said hydrolysis 5-7;(4) Stable in the pH range of 4.5-6;(5) Optimal temperature range for activity 35.degree.-40.degree. C.;(6) thermal stability up to about 35.degree. C.;(7) Activated by surface-active agents, but not substantially inhibited by such surface-active agents in concentration up to 5%;(8) Molecular weight about 1000,000 to 120,000;(9) Isoelectric point about pH 3.84.
摘要翻译： 从青霉菌分离的脂肪酶，其特征在于以下性质：（1）有效地水解具有4-18个碳原子的脂肪酸的甘油三酯; （2）用这种脂肪酶水解这种甘油三酸酯每100摩尔脂肪酸产生至少5摩尔甘油; （3）所述水解5-7的最佳pH范围; （4）在4.5-6的pH范围内稳定; （5）最适温度范围为35°-40°C。 （6）热稳定性高达约35℃。 （7）表面活性剂活化，但浓度高达5％时，这些表面活性剂基本上不被抑制; （8）分子量约为1000,000至120,000; （9）等电点约pH3.84。
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4.发明授权
公开(公告)号：US4839279A
公开(公告)日：1989-06-13
申请号：US749425
申请日：1985-06-27
CPC分类号： C12Q1/62 , C12Q1/26 , C12Q1/54 , C12Q1/60 , Y10S436/811 , Y10S436/815 , Y10S436/825 , Y10T436/103332 , Y10T436/144444 , Y10T436/146666 , Y10T436/25125
摘要： A novel enzyme of bilirubin oxidase and a conventional enzyme of laccase are found to oxidize bilirubin to biliverdin without formation of hydrogen peroxide, thereby permitting the content of conjugated bilirubin in biological fluid to be quantitatively determined by reacting therewith the bilirubin oxidase of a microorganism of genus Myrothecium origin, and also permitting the content of total bilirubin in biological fluid to be quantitatively determined by reacting therewith such a bilirubin oxidase or laccase in the presence of a specific additive compound.
摘要翻译： 发现胆红素氧化酶的新型酶和漆酶的常规酶将胆红素氧化成胆绿素而不形成过氧化氢，从而通过与生物液体中的微生物的胆红素氧化酶反应来定量地测定生物液中的共轭胆红素含量 通过与特定的添加剂化合物的存在下的胆红素氧化酶或漆酶反应，可以定量测定生物体液中总胆红素的含量。
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公开(公告)号：US4636465A
公开(公告)日：1987-01-13
申请号：US601252
申请日：1984-04-17
申请人： Nobuya Itoh , Kuniyoshi Matsunaga
发明人： Nobuya Itoh , Kuniyoshi Matsunaga
CPC分类号： C12Q1/61 , C12Q1/32 , C12Q1/44 , C12Q1/48 , G01N2333/904 , G01N2333/9121 , Y10S435/81
摘要： A method for determination of glycerol by using a reagent system comprising glycerol dehydrogenase and a pyridine nucleotide coenzyme, characterized in that an enzyme selected from the group consisting of triokinase and dihydroxyacetone kinase is incorporated into the reagent system to eliminate the formed dihydroxyacetone or D-glyceraldehyde in the assay system.
摘要翻译： 通过使用包含甘油脱氢酶和吡啶核苷酸辅酶的试剂系统测定甘油的方法，其特征在于将选自三角蛋白酶和二羟基丙酮激酶的酶掺入试剂系统中以除去形成的二羟基丙酮或D-甘油醛 在测定系统中。
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公开(公告)号：US4892816A
公开(公告)日：1990-01-09
申请号：US136522
申请日：1987-12-22
CPC分类号： C12Q1/60 , Y10S435/81
摘要： An enzymatic method for measurement of cholesterol comprises incubating:(a) a test sample;(b) a cholesterol dehydrogenase;(c) an oxidizing agent selected from the group consisting of nicotinamide - adenine dinucleotide (NAD) and nicotinamide - adenine dinucleotide phosphate (NADP); and(d) a surfactantand measuring the resulting detectable oxidized and reduced products kinetically.A composition for the kinetic measurement of cholesterol comprises:(a) a cholesterol dehydrogenase;(b) an oxidizing agent selected from the group consisting of nicotinamide - adenine dinucleotide (NAD) and nicotinamide - adenine dinucleotide phosphate (NADP); and(c) a surfactant.
摘要翻译： 用于测量胆固醇的酶法包括孵育：（a）测试样品; （b）胆固醇脱氢酶; （c）选自烟酰胺 - 腺嘌呤二核苷酸（NAD）和烟酰胺 - 腺嘌呤二核苷酸磷酸（NADP）的氧化剂; 和（d）表面活性剂并动态地测量所得可检测的氧化和还原产物。 用于胆固醇动力学测定的组合物包括：（a）胆固醇脱氢酶; （b）选自烟酰胺 - 腺嘌呤二核苷酸（NAD）和烟酰胺 - 腺嘌呤二核苷酸磷酸（NADP）的氧化剂; 和（c）表面活性剂。
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公开(公告)号：US4579822A
公开(公告)日：1986-04-01
申请号：US598746
申请日：1984-04-10
申请人： Nobuya Itoh , Kuniyoshi Matsunaga
发明人： Nobuya Itoh , Kuniyoshi Matsunaga
CPC分类号： C12N9/12 , Y10S435/911
摘要： A process for producing dihydroxyacetone kinase comprises the cultivation of the DHAK-producing strains of genus Schizosaccharomyces, and the purification of dihydroxyacetone kinase from the culture.
摘要翻译： 制备二羟基丙酮激酶的方法包括培养粟酒裂殖酵母属的DHAK生产菌株，并从培养物中纯化二羟基丙酮激酶。
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8.发明授权
公开(公告)号：US4554249A
公开(公告)日：1985-11-19
申请号：US436385
申请日：1982-10-25
CPC分类号： C12Q1/62 , C12Q1/26 , C12Q1/54 , C12Q1/60 , Y10S436/811 , Y10S436/815 , Y10S436/825 , Y10T436/103332 , Y10T436/144444 , Y10T436/146666 , Y10T436/25125
摘要： A novel enzyme of bilirubin oxidase produced by a genus Myrothecium or genus Coprinus origin microorganism and a conventional enzyme of laccase are found, in the presence of a specific additive compound, e.g. a surface active agent, aromatic carboxylic acid, sulfa drug or protease, to oxidize both conjugated and unconjugated bilirubin in biological fluid to biliverdin without formation of hydrogen peroxide, such that in the case of conventional enzymatic methods of the quantitative determination of glucose, cholesterol, neutral fats, free fatty acids, phospholipids or uric acid all existing together with bilirubin in biological fluid, the usual interference with such determination, as otherwise caused by bilirubin coexisting in such fluid, can be prevented by adding such a bilirubin oxidase or laccase together with such a specific additive compound to the determinative reaction system.
摘要翻译： 在特定的添加剂化合物的存在下，发现了由黑曲霉属或鬼伞属起源微生物和常规酶漆酶产生的胆红素氧化酶的新型酶。 表面活性剂，芳族羧酸，磺胺药物或蛋白酶，以将生物液体中的共轭和非共轭胆红素氧化成胆绿素而不形成过氧化氢，使得在常规的酶法测定葡萄糖，胆固醇， 中性脂肪，游离脂肪酸，磷脂或尿酸都与生物液体中的胆红素一起存在，通过将这种胆红素共存在这种流体中的其他方式引起的这种测定的通常干扰可以通过将这样的胆红素氧化酶或漆酶与 这种特定的添加剂化合物与决定性反应体系。
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