-
公开(公告)号：US5082781A
公开(公告)日：1992-01-21
申请号：US611480
申请日：1990-11-09
CPC分类号： C12N9/2425
摘要： A novel .beta.-amylase gene is provided. This gene is useful for production of a thermophilic .beta.-amylase obtained from Clostridium thermosulfurogenes on a large scale, especially from genetically engineered microorganisms introduced with this gene. B. subtilis was transformed with a plasmid pNK1 containing the above gene and the transformed B. subtilis cells produced .beta.-amylase having the same thermostability as that of .beta.-amylase obtained from C. thermosulfurogenes.
-
公开(公告)号：US4994380A
公开(公告)日：1991-02-19
申请号：US928125
申请日：1986-11-07
CPC分类号： C12N9/2417 , C12N15/75 , Y10S435/833
摘要： An improved vector for expression in Bacillus brevis having:(1) a nucleotide sequence (a) represented by a general formula MNOACP;(2) a nucleotide sequence (b) located in the downstream of the nucleotide sequence (a) and represented by a general formula QRSWXY;(3) a nucleotide sequence (c) located in the downstream of the nucleotide sequence (b) and acting as a binding site to ribosome in the cell of Bacillus brevis;(4) a nucleotide sequence (d) located in the downstream of the nucleotide sequence (c) and acting as a translation initiation condon in the cell of Bacillus brevis; and(5) a gene directly connected with the nucleotide sequence (d) and to express in the cell of Bacillus brevis;wherein M represents G or T; N represents C, T or A; O represents A, C or T; P represents T or G; Q represents T or A; R represents T or A; S represents T, C or A; W represents A or G; X represents A or C; and Y represents T or G; and furthermore, wherein A represents adenine, C cytosine, G guanine and T thymine.
摘要翻译： 一种用于在短芽孢杆菌中表达的改良载体，其具有：（1）由通式MNOACP表示的核苷酸序列（a） （2）位于核苷酸序列（a）的下游并由通式QRSWXY表示的核苷酸序列（b）; （3）位于核苷酸序列（b）下游的核苷酸序列（c），并且作为芽孢杆菌细胞中核糖体的结合位点; （4）位于核苷酸序列（c）下游的核苷酸序列（d），并在短芽孢杆菌细胞中作为翻译起始细菌; 和（5）与核苷酸序列（d）直接连接并在短芽孢杆菌细胞中表达的基因; 其中M表示G或T; N表示C，T或A; O表示A，C或T; P表示T或G; Q表示T或A; R表示T或A; S表示T，C或A; W代表A或G; X表示A或C; Y表示T或G; 此外，其中A表示腺嘌呤，C胞嘧啶，G鸟嘌呤和T胸腺嘧啶。
-
公开(公告)号：US08648183B2
公开(公告)日：2014-02-11
申请号：US10505171
申请日：2003-02-28
申请人： Kanako Suzuki , Norihiro Tsukagoshi
发明人： Kanako Suzuki , Norihiro Tsukagoshi
CPC分类号： C12N15/80
摘要： It is intended to provide base sequences participating in the regulation of the expression of a promoter. It is also intended to modify a promoter based on the base sequence data to give a modified promoter having a high expression activity. Namely, a modified promoter constructed by inserting a first DNA fragment containing CCAATNNNNNN (SEQ ID NO:1) (a first base sequence) and a second DNA fragment containing CGGNNNNNNNNNGG (SEQ ID NO:2) (a second base sequence) into a promoter functioning in a filamentous fungus, wherein N in the base sequence denotes A (adenine), G (guanine), C(cytosine), or T (thymine).
摘要翻译： 旨在提供参与启动子表达调节的碱基序列。 还旨在基于碱基序列数据修饰启动子以产生具有高表达活性的修饰的启动子。 即，通过将含有CCAATNNNNNN（SEQ ID NO：1）（第一碱基序列）的第一DNA片段和含有CGGNNNNNNNNNNNGG（SEQ ID NO：2）（第二碱基序列）的第二DNA片段）（第二碱基序列）插入启动子构建的修饰启动子 在丝状真菌中起作用，其中碱基序列中的N表示A（腺嘌呤），G（鸟嘌呤），C（胞嘧啶）或T（胸腺嘧啶）。
-
公开(公告)号：US20060234338A1
公开(公告)日：2006-10-19
申请号：US10532064
申请日：2003-10-20
申请人： Kanako Suzuki , Norihiro Tsukagoshi
发明人： Kanako Suzuki , Norihiro Tsukagoshi
CPC分类号： C12N9/2451 , C12N9/2408 , C12N15/80 , C12Y204/01139 , C12Y302/0102
摘要： It is intended to provide a host microorganism whereby the expression of a gene encoding a target protein can be effectively elevated in the case of transferring the gene. It is also intended to provide a transformant whereby a target protein can be produced at a high efficiency. It is further intended to provide a production process whereby a target protein can be produced at a high productivity. A microorganism which belongs to Eumycota and lacks the major isomaltose synthase. A gene encoding a target protein is transferred into the microorganism to give a transformant. Then the transformant is cultured under conditions allowing the expression of the transferred gene to thereby produce the protein.
摘要翻译： 旨在提供宿主微生物，其中在转移基因的情况下可以有效地提高编码靶蛋白的基因的表达。 还旨在提供一种可以以高效率生产靶蛋白的转化体。 进一步的目的是提供一种可以高生产率生产靶蛋白的生产方法。 属于真菌纲的微生物，缺少主要的异麦芽糖合成酶。 将编码靶蛋白的基因转移到微生物中，得到转化体。 然后将转化体在允许转移的基因表达从而产生蛋白质的条件下培养。
-
公开(公告)号：US20050170453A1
公开(公告)日：2005-08-04
申请号：US10505171
申请日：2003-02-28
申请人： Kanako Suzuki , Norihiro Tsukagoshi
发明人： Kanako Suzuki , Norihiro Tsukagoshi
CPC分类号： C12N15/80
摘要： It is intended to provide base sequences participating in the regulation of the expression of a promoter. It is also intended to modify a promoter based on the base sequence data to give a modified promoter having a high expression activity. Namely, a modified promoter constructed by inserting a first DNA fragment containing CCAATNNNNNN (a first base sequence) and a second DNA fragment containing CGGNNNNNNNNNGG (a second base sequence) into a promoter functioning in a filamentous fungus, wherein N in the base sequence denotes A (adenine), G (guanine), C(cytosine), or T (thymine).
摘要翻译： 旨在提供参与启动子表达调节的碱基序列。 还旨在基于碱基序列数据修饰启动子以产生具有高表达活性的修饰的启动子。 即，通过将含有CCAATNNNNNN（第一碱基序列）的第一DNA片段和含有CGGNNNNNNNNNNGG（第二碱基序列）的第二DNA片段）插入到丝状真菌中起作用的启动子中构建的修饰启动子，其中碱基序列中的N表示A （腺嘌呤），G（鸟嘌呤），C（胞嘧啶）或T（胸腺嘧啶）。
-
公开(公告)号：US07341848B2
公开(公告)日：2008-03-11
申请号：US10532064
申请日：2003-10-20
申请人： Kanako Suzuki , Norihiro Tsukagoshi
发明人： Kanako Suzuki , Norihiro Tsukagoshi
CPC分类号： C12N9/2451 , C12N9/2408 , C12N15/80 , C12Y204/01139 , C12Y302/0102
摘要： It is intended to provide a host microorganism whereby the expression of a gene encoding a target protein can be effectively elevated in the case of transferring the gene. It is also intended to provide a transformant whereby a target protein can be produced at a high efficiency. It is further intended to provide a production process whereby a target protein can be produced at a high productivity. A microorganism which belongs to Eumycota and lacks the major isomaltose synthase. A gene encoding a target protein is transferred into the microorganism to give a transformant. Then the transformant is cultured under conditions allowing the expression of the transferred gene to thereby produce the protein.
摘要翻译： 旨在提供宿主微生物，其中在转移基因的情况下可以有效地提高编码靶蛋白的基因的表达。 还旨在提供一种可以以高效率生产靶蛋白的转化体。 进一步的目的是提供一种可以高生产率生产靶蛋白的生产方法。 属于真菌纲的微生物，缺少主要的异麦芽糖合成酶。 将编码靶蛋白的基因转移到微生物中，得到转化体。 然后将转化体在允许转移的基因表达从而产生蛋白质的条件下培养。
-
-
-
-
-
搜索结果
6 条记录 (耗时 0.021 秒)
