-
公开(公告)号：US5082781A
公开(公告)日：1992-01-21
申请号：US611480
申请日：1990-11-09
CPC分类号： C12N9/2425
摘要： A novel .beta.-amylase gene is provided. This gene is useful for production of a thermophilic .beta.-amylase obtained from Clostridium thermosulfurogenes on a large scale, especially from genetically engineered microorganisms introduced with this gene. B. subtilis was transformed with a plasmid pNK1 containing the above gene and the transformed B. subtilis cells produced .beta.-amylase having the same thermostability as that of .beta.-amylase obtained from C. thermosulfurogenes.
-
公开(公告)号：US4994380A
公开(公告)日：1991-02-19
申请号：US928125
申请日：1986-11-07
CPC分类号： C12N9/2417 , C12N15/75 , Y10S435/833
摘要： An improved vector for expression in Bacillus brevis having:(1) a nucleotide sequence (a) represented by a general formula MNOACP;(2) a nucleotide sequence (b) located in the downstream of the nucleotide sequence (a) and represented by a general formula QRSWXY;(3) a nucleotide sequence (c) located in the downstream of the nucleotide sequence (b) and acting as a binding site to ribosome in the cell of Bacillus brevis;(4) a nucleotide sequence (d) located in the downstream of the nucleotide sequence (c) and acting as a translation initiation condon in the cell of Bacillus brevis; and(5) a gene directly connected with the nucleotide sequence (d) and to express in the cell of Bacillus brevis;wherein M represents G or T; N represents C, T or A; O represents A, C or T; P represents T or G; Q represents T or A; R represents T or A; S represents T, C or A; W represents A or G; X represents A or C; and Y represents T or G; and furthermore, wherein A represents adenine, C cytosine, G guanine and T thymine.
摘要翻译： 一种用于在短芽孢杆菌中表达的改良载体，其具有：（1）由通式MNOACP表示的核苷酸序列（a） （2）位于核苷酸序列（a）的下游并由通式QRSWXY表示的核苷酸序列（b）; （3）位于核苷酸序列（b）下游的核苷酸序列（c），并且作为芽孢杆菌细胞中核糖体的结合位点; （4）位于核苷酸序列（c）下游的核苷酸序列（d），并在短芽孢杆菌细胞中作为翻译起始细菌; 和（5）与核苷酸序列（d）直接连接并在短芽孢杆菌细胞中表达的基因; 其中M表示G或T; N表示C，T或A; O表示A，C或T; P表示T或G; Q表示T或A; R表示T或A; S表示T，C或A; W代表A或G; X表示A或C; Y表示T或G; 此外，其中A表示腺嘌呤，C胞嘧啶，G鸟嘌呤和T胸腺嘧啶。
-
公开(公告)号：US5411886A
公开(公告)日：1995-05-02
申请号：US112630
申请日：1993-08-27
申请人： Shigezo Udaka , Kenji Sakaguchi , Hideo Yamagata , Koen Dekker
发明人： Shigezo Udaka , Kenji Sakaguchi , Hideo Yamagata , Koen Dekker
IPC分类号： C12N9/90 , C12N1/19 , C12N9/92 , C12N15/09 , C12N15/61 , C12P19/24 , C12R1/01 , C12R1/08 , C12R1/125 , C12R1/465 , C12R1/865 , C12N1/21 , C12N15/63
摘要： A xylose isomerase gene from Thermus bacteria, such as Thermos aquaticus (ATCC 27634) and a gene having 60% or more of homology to the nucleotide sequence of Thermus aquaticus xylose isomerase gene of FIG. 1-3. A xylose isomerase from Thermus aquaticus characterized in that the xylose isomerase has the optimal pH of about 7, the stable pH range of from about 6 to 8.5, the optimal temperature of about 95.degree. C. and the molecular weight of about 44,000, and is stabilized with manganese or magnesium. A process for preparing a xylose isomerase comprising transforming a microorganism with a plasmid containing the above gene and a promoter, culturing the transformed microorganism and harvesting the produced xylose isomerase. A process for preparing fructose comprising isomerization of glucose to fructose in the presence of the above xylose isomerase.
摘要翻译： 来自Thermus细菌的木糖异构酶基因，例如Thermos aquaticus（ATCC 27634）和与图1的Thermus aquaticus木糖异构酶基因的核苷酸序列具有60％以上同源性的基因。 1-3。 水生栖热菌属的木糖异构酶，其特征在于木糖异构酶具有约7的最佳pH，约6至8.5的稳定pH范围，约95℃的最佳温度和约44,000的分子量，并且为 用锰或镁稳定。 一种制备木糖异构酶的方法，包括用含有上述基因的质粒和启动子转化微生物，培养转化的微生物并收获所产生的木糖异构酶。 一种制备果糖的方法，包括在上述木糖异构酶存在下将葡萄糖异构化成果糖。
-
公开(公告)号：US5665570A
公开(公告)日：1997-09-09
申请号：US384545
申请日：1995-02-07
申请人： Hideo Yamagata , Shigezo Udaka , Yasushi Inoue , Hiroko Tada , Susumu Iwasa
发明人： Hideo Yamagata , Shigezo Udaka , Yasushi Inoue , Hiroko Tada , Susumu Iwasa
IPC分类号： C07K16/00 , C07K16/18 , C07K16/40 , C12N15/13 , C12N15/75 , C12P21/08 , C07H21/04 , C12N1/20
CPC分类号： C12N15/75 , C07K16/00 , C07K16/18 , C07K16/40 , C07K2319/00
摘要： A bacterium of the genus Bacillus is transformed by introducing a vector incorporating DNA encoding an antibody; the resulting transformant is cultivated in a medium to produce and accumulate the antibody in an active form in the culture liquid. According to this method, a recombinant antibody can be easily produced in an active form in large amounts, owing to success in secretory expression using a bacterium of the genus Bacillus.
摘要翻译： 通过引入含有编码抗体的DNA的载体来转化芽孢杆菌属的细菌; 将所得转化体在培养基中培养，以活性形式产生并积累培养液中的抗体。 根据该方法，由于使用芽孢杆菌属的细菌的分泌表达的成功，重组抗体可以大量容易地以活性形式产生。
-
公开(公告)号：US4560748A
公开(公告)日：1985-12-24
申请号：US500908
申请日：1983-06-03
申请人： Shigezo Udaka , Shigeyoshi Miyashiro , Kazuo Hirayama , Toshihiko Ando , Asao Murai , Tsuyoshi Shiio , Takao Kida , Hiroshiro Shibai
发明人： Shigezo Udaka , Shigeyoshi Miyashiro , Kazuo Hirayama , Toshihiko Ando , Asao Murai , Tsuyoshi Shiio , Takao Kida , Hiroshiro Shibai
IPC分类号： C12P21/02 , A61K31/70 , A61K31/7042 , A61K31/7052 , A61P31/04 , A61P31/12 , A61P35/00 , C07H17/02 , C12P19/28 , C07H17/00
摘要： A streptothricin-group compound having the formula: ##STR1## in which R represents ##STR2## is disclosed along with methods for producing this compound and a microorganism useful in its production.
摘要翻译： 公开了具有下式的其中R表示
+ TR 的下式的链霉菌素基化合物以及用于生产该化合物的方法和用于其生产的微生物。 -
公开(公告)号：US4946789A
公开(公告)日：1990-08-07
申请号：US43459
申请日：1987-04-28
申请人： Shigezo Udaka , Hiroaki Takagi , Kiyoshi Kadowaki
发明人： Shigezo Udaka , Hiroaki Takagi , Kiyoshi Kadowaki
CPC分类号： C12N9/2417 , C12N15/75 , C12R1/08
摘要： Bacillus brevis strains which produce a large amount of protein but no protease out of the cells are disclosed. These strains are highly useful as hosts in genetic engineering.
摘要翻译： 公开了产生大量蛋白质但不含细胞蛋白酶的短芽孢杆菌菌株。 这些菌株在基因工程中作为宿主非常有用。
-
公开(公告)号：US07037684B2
公开(公告)日：2006-05-02
申请号：US10221677
申请日：2001-03-13
申请人： Shigezo Udaka , Seiji Sato , Toshiyuki Kudo , Shusaku Oka , Naohiko Higashikuni , Masaaki Kondo
发明人： Shigezo Udaka , Seiji Sato , Toshiyuki Kudo , Shusaku Oka , Naohiko Higashikuni , Masaaki Kondo
CPC分类号： C12P21/02 , C07K14/32 , C07K14/62 , C07K2319/02 , C07K2319/035 , C07K2319/75 , C12N9/90 , C12N15/625 , C12N15/75
摘要： The invention relates to a DNA that enables co-expression of a protein disulfide isomerase and a polypeptide having disulfide bonds. The invention further relates to a process of producing polypeptides using the DNA and a process of enhancing the efficiency of formation of correct disulfide bond in an expression system of a gene recombinant polypeptide having disulfide bonds.
摘要翻译： 本发明涉及能够共表达蛋白质二硫键异构酶和具有二硫键的多肽的DNA。 本发明还涉及使用该DNA产生多肽的方法和在具有二硫键的基因重组多肽的表达系统中提高正确二硫键形成的效率的方法。
-
公开(公告)号：US5714346A
公开(公告)日：1998-02-03
申请号：US507313
申请日：1995-08-22
申请人： Shigezo Udaka , Tsutomu Kajino , Yoko Saito , Masana Hirai , Yukio Yamada , Fumihiko Hoshino
发明人： Shigezo Udaka , Tsutomu Kajino , Yoko Saito , Masana Hirai , Yukio Yamada , Fumihiko Hoshino
CPC分类号： C07K14/61 , C12N15/75 , C07K2319/02
摘要： A host-vector system suitable for production of human growth hormone (hGH) and a process for production of hGH using the same are provided. As an hGH expression plasmid, a recombinant DNA wherein a DNA coding for hGH is linked to the 3'-terminal of a DNA containing a promoter region derived from Bacillus brevis is provided, and as a host, especially a mutant Bacillus brevis substantially not exhibiting protease activity to hGH is provided. A microorganism obtained by transforming said host with said hGH expression plasmid efficiently produces hGH when it is cultured.
摘要翻译： PCT No.PCT / JP94 / 00269 Sec。 371日期：1995年8月22日 102（e）日期1995年8月22日PCT 1994年2月22日PCT PCT。 WO94 / 19474 PCT公开号 日期1995年9月1日提供适用于生产人生长激素（hGH）的宿主载体系统和使用其的生产hGH的方法。 作为hGH表达质粒，提供了重组DNA，其中编码hGH的DNA与含有源自短芽孢杆菌的启动子区的DNA的3'-末端连接，作为宿主，特别是基本上不显示的突变体短芽孢杆菌 提供了对hGH的蛋白酶活性。 通过用所述hGH表达质粒转化所述宿主获得的微生物在培养时有效地产生hGH。
-
公开(公告)号：US5783417A
公开(公告)日：1998-07-21
申请号：US549757
申请日：1996-02-05
申请人： Toshi Komurasaki , Kiyoshi Nakazawa , Hitoshi Toyoda , Masayoshi Takahashi , Daisuke Uchida , Kazunori Hanada , Shigezo Udaka
发明人： Toshi Komurasaki , Kiyoshi Nakazawa , Hitoshi Toyoda , Masayoshi Takahashi , Daisuke Uchida , Kazunori Hanada , Shigezo Udaka
CPC分类号： C07K14/4703 , C07K14/47 , C07K14/52 , A61K38/00
摘要： A DNA fragment encoding a novel human-derived tumor cell growth inhibitor can be obtained from a cDNA library originating from human colon tumor cell, using as a DNA probe a DNA fragment encoding mouse human-derived tumor cell growth inhibitor. An expression plasmid bearing a promoter originating from Bacillus brevis, a signal peptide originating from Bacillus brevis and a DNA fragment encoding human-derived tumor cell growth inhibitor downstream of the signal peptide is constructed and Bacillus brevis is transformed by the expression plasmid. The resulting transformant is incubated to secret the novel inhibitor out of the cell so that the novel inhibitor can be produced efficiently.
摘要翻译： PCT No.PCT / JP94 / 00895 Sec。 371日期：1996年2月5日 102（e）日期1996年2月5日PCT Filed 1994年6月2日PCT公布。 公开号WO94 / 29340 1995年12月22日编码新型人源肿瘤细胞生长抑制剂的DNA片段可以从源自人结肠肿瘤细胞的cDNA文库获得，使用DNA探针作为编码小鼠来源于人的肿瘤细胞生长抑制剂的DNA片段。 构建携带源自短芽孢杆菌的启动子的表达质粒，来自短芽孢杆菌的信号肽和在信号肽下游编码人源肿瘤细胞生长抑制剂的DNA片段，并通过表达质粒转化短杆菌。 将所得转化体孵育以将新型抑制剂从细胞中分离出来，使得可以有效地生产新的抑制剂。
-
公开(公告)号：US4507282A
公开(公告)日：1985-03-26
申请号：US502531
申请日：1983-06-10
摘要： A high molecular weight peptide antibiotic designated as AN-7D which is effective as an agent.
摘要翻译： 称为AN-7D的高分子量肽抗生素，作为药剂有效。
-
-
-
-
-
-
-
-
-
搜索结果
13 条记录 (耗时 0.02 秒)
