公开(公告)号：US07366334B2

公开(公告)日：2008-04-29

申请号：US10627979

申请日：2003-07-28

申请人： Hideo Yokota ,  Satoko Takemoto ,  Taketoshi Mishima ,  Akitake Makinouchi ,  Ryutaro Himeno

发明人： Hideo Yokota ,  Satoko Takemoto ,  Taketoshi Mishima ,  Akitake Makinouchi ,  Ryutaro Himeno

IPC分类号： G06K9/00

CPC分类号： G06K9/4638 ,  G06K9/3233 ,  G06K2009/366 ,  G06K2209/05 ,  G06T7/11 ,  G06T7/12 ,  G06T7/187 ,  G06T2200/04 ,  G06T2207/20104 ,  G06T2207/20116 ,  G06T2207/30004

摘要： Assuming that there are three continuous frames, i.e, cross sectional images of an organism, a dummy region of interest (ROI) is specified manually in the first frame, a temporary ROI is set in the second frame at the same position as that of the dummy ROI in the first frame. Whether a specific region in the second frame is inside or outside of a true ROI is judged based on the initial judgment criterion and values of pixels in the specific region. Whether a specific region in the third frame is inside or outside of the true ROI is judged based on values of pixels of regions that have been judged to be inside the region of interest in the second frame.

摘要翻译： 假设存在三个连续帧，即生物体的横截面图像，在第一帧中手动指定感兴趣的虚拟区域（ROI），在第二帧中设置与第二帧相同位置的临时ROI 虚拟ROI在第一帧。 基于特定区域中的初始判定基准和像素值，判断第二帧中的特定区域是否在真实ROI内部或外部。 基于在第二帧中被判断为在感兴趣区域内的区域的像素的值来判断第三帧中的特定区域是否在真实ROI内部或外部。

公开(公告)号：US20060200318A1

公开(公告)日：2006-09-07

申请号：US11218466

申请日：2005-09-06

申请人： So-ichiro Morishita ,  Hideo Yokota ,  Ryutaro Himeno ,  Taketoshi Mishima

发明人： So-ichiro Morishita ,  Hideo Yokota ,  Ryutaro Himeno ,  Taketoshi Mishima

IPC分类号： G06F19/00 ,  G01N31/00

CPC分类号： G01N21/6428 ,  G01N2201/1293

摘要： It is an object of the invention to provide an estimation method of fluorescent dye's concentration from multiple fluorescence, where the accurate estimation of the fluorescent dye's concentration of each fluorescent dye from multiple fluorescence is made possible and the separation of multiple fluorescence which is difficult in the prior art is made possible. In the estimation method of fluorescent dye's concentration from multiple fluorescence where the fluorescent dye's concentration from measured multiple fluorescence, independent component analysis is performed to the spectrum of fluorescent dye where fluorescent dye's concentration is known to derive the intensity of an independent component, regression analysis is performed by using the derived intensity of the independent component as a variable to estimate the fluorescent dye's concentration function of the fluorescent dye where the fluorescent dye's concentration is known, and the concentration of fluorescent dye is estimated from the measured multiple fluorescence based on the estimated fluorescent dye's concentration function.

摘要翻译： 本发明的一个目的是提供一种荧光染料从多个荧光的浓度的估计方法，其中可以准确地估计荧光染料从多个荧光的荧光染料的浓度，并且在多个荧光中难以分离多个荧光 现有技术成为可能。 在荧光染料浓度从荧光染料浓度测量多个荧光的荧光染料浓度的估计方法中，对已知荧光染料浓度的荧光染料的光谱进行独立成分分析，得出独立成分的强度，回归分析为 通过使用独立成分的衍生强度作为变量来进行荧光染料的荧光染料的浓度函数的荧光染料的浓度的测定，荧光染料的浓度是已知的，荧光染料的浓度是根据测定的多个荧光估计的 染料的浓度功能。

公开(公告)号：US20080267469A1

公开(公告)日：2008-10-30

申请号：US12047186

申请日：2008-03-12

申请人： Kuniaki Kawabata ,  Yuta Komori ,  Taketoshi Mishima ,  Hajime Asama ,  Kei Takeda

发明人： Kuniaki Kawabata ,  Yuta Komori ,  Taketoshi Mishima ,  Hajime Asama ,  Kei Takeda

IPC分类号： G06K9/00

CPC分类号： G06T7/0004 ,  G06T2207/10152

摘要： The present invention provides assistance for visual observation based counting of particles or crystals. In an image of a specimen of an unidentified sample, particles are counted from an image obtained as a result of binarization using a method such as the discriminant analysis method, for example, and crystals are counted from two images using the difference between the images according with different imaging conditions. As an example, in the particle counting, such as a two-step noise removal is performed, and also, for the crystals, alignment and aspect ratio calculation are performed. In particular, the present invention can provide assistance for the dispersion staining method in which asbestos crystals are visually searched for using a phase-contrast microscope.

摘要翻译： 本发明提供对基于目视观察的颗粒或晶体计数的辅助。 在未鉴定样品的样本的图像中，使用例如判别分析方法的方法从作为二值化结果获得的图像中计数颗粒，并且使用图像之间的差异从两个图像计数晶体 具有不同的成像条件。 例如，在粒子计数中，例如进行两步噪声除去，并且对于晶体，进行对准和纵横比计算。 特别地，本发明可以对使用相差显微镜目视检索石棉晶体的分散染色方法提供帮助。