High throughput multiplex spectroscopy

    公开(公告)号:US11740127B2

    公开(公告)日:2023-08-29

    申请号:US17481542

    申请日:2021-09-22

    CPC classification number: G01J3/433 G01J3/2846 G01N21/314 G01N2201/1293

    Abstract: A method for measuring a property of radiation from different sources such as moving particles or different spatial locations on each particle includes providing a spatial modulator common to all of the sources having a sequence of configurations, each of which configurations causes the radiation flux to pass along paths to respective modulation ports and cycling the common spatial modulator past each of the modulation ports so that the sequence of configurations is applied to each modulation port. The sequence of configurations comprises an ordered array of optical elements on a substrate. In one embodiment, the modulator is arranged in a circle around an axis of rotation of a rotating singulation disk. At least one source is a reference source which has not interacted with the source to be analyzed and the sample and reference sources are compared.

    Method of predicting a performance characteristic of a plant or yeast hydrolysate and its use

    公开(公告)号:US11650159B2

    公开(公告)日:2023-05-16

    申请号:US16694789

    申请日:2019-11-25

    Abstract: The present invention relates to a method of predicting a performance characteristic of a plant or yeast hydrolysate, wherein a plant or yeast hydrolysate sample is measured with 2D fluorescence spectroscopy in powder form. Said method comprises the steps for providing a model based on a predetermined value of a manufacturing parameter of interest. For this purpose a training set consisting of predetermined manufacturing parameter of interest (e.g volumetric productivity parameter, virus titer or cell number) and fluorescence spectroscopic data is used. The fluorescence spectroscopic data is correlated to the values of the manufacturing parameter of interest to obtain a calibration model/model parameters by applying multivariate data analysis. This calibration model is being used to predict the manufacturing parameter of interest for new samples dedicated for the manufacturing process. This prediction is used for a decision to accept or reject the lot which corresponds to the respective sample for use in the manufacturing process or for further evaluation depending on the pre-defined range of the manufacturing parameter of interest. The invention further relates to a method for preparation of a cell culture medium, preferably an animal protein free cell culture medium, a method for cultivating cells, a method for producing a recombinant target protein, a method for producing an immunogenic composition, whereby the above method of predicting a performance characteristic has been used for selecting the plant or yeast hydrolysate to be used in the manufacturing process.

    Comparative discrimination spectral detection system and method for the identification of chemicals with overlapping spectral signatures

    公开(公告)号:US09857295B2

    公开(公告)日:2018-01-02

    申请号:US14966807

    申请日:2015-12-11

    CPC classification number: G01N21/3504 G01N21/35 G01N2201/1293

    Abstract: A comparative discrimination spectral detection (CDSD) system for the identification of chemicals with overlapping spectral signatures, including: a radiation source for delivering radiation to a sample; a radiation collector for collecting radiation from the sample; a plurality of beam splitters for splitting the radiation collected from the sample into a plurality of radiation beams; a plurality of low-resolution optical filters for filtering the plurality of radiation beams; a plurality of radiation detectors for detecting the plurality filtered radiation beams; and a processor for: receiving a set of reference spectra related to a set of target chemicals and generating a set of base vectors for the set of target chemicals from the set of reference spectra, wherein the set of base vectors define a geometrical shape in a configuration space; receiving a set of filtered test spectra from the plurality of radiation detectors and generating a set of test vectors in the configuration space from the set of filtered test spectra; assessing a geometrical relationship of the set of test vectors and the geometrical shape defined by the set of base vectors in the configuration space; and based on the assessed geometrical relationship, establishing a probability that a given test spectrum or spectra matches a given reference spectrum or spectra.

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