公开(公告)号：US20070130643A1

公开(公告)日：2007-06-07

申请号：US11626131

申请日：2007-01-23

申请人： Hiroaki KISAKA ,  Takao Kida

发明人： Hiroaki KISAKA ,  Takao Kida

IPC分类号： A01H5/00 ,  A01H1/00

CPC分类号： C12N9/0004 ,  C12N9/0016 ,  C12N9/93 ,  C12N15/8241 ,  C12N15/8251 ,  C12N15/8261 ,  C12Y104/01002 ,  C12Y104/01004 ,  Y02A40/146

摘要： The object of the present invention is to provide transgenic plants which accumulate free amino acids, particularly at least one amino acid selected from among glutamic acid, asparagine, aspartic acid, serine, threonine, alanine and histidine accumulated in a large amount, in the edible parts thereof, and a method of producing them are provided. The other object of the present invention is to provide a method of increasing the yielding of potato and to provide a transgenic potato of which yielding can be increased. In the present invention, a sequence encoding glutamate dehydrogenase (GDH) gene is introduced into a plant together with a suitable regulatory sequence to express it in a plant cell, and thereby the GDH gene is excessively expressed.

摘要翻译： 本发明的目的是提供一种转基因植物，其在食用中积累游离氨基酸，特别是至少一种选自谷氨酸，天冬酰胺，天冬氨酸，丝氨酸，苏氨酸，丙氨酸和组氨酸中的至少一种氨基酸 部分，以及它们的制造方法。 本发明的另一个目的是提供一种增加马铃薯产量的方法，并且提供可以提高产量的转基因马铃薯。 在本发明中，将编码谷氨酸脱氢酶（GDH）基因的序列与合适的调节序列一起导入植物，以在植物细胞中表达，从而过度表达GDH基因。

公开(公告)号：US20110166371A1

公开(公告)日：2011-07-07

申请号：US12976526

申请日：2010-12-22

申请人： Hiroaki KISAKA ,  Yaqin Lang ,  Ryuji Sugiyama ,  Tetsuya Miwa ,  Susumu Yazawa

发明人： Hiroaki KISAKA ,  Yaqin Lang ,  Ryuji Sugiyama ,  Tetsuya Miwa ,  Susumu Yazawa

IPC分类号： A01H5/00 ,  C12N15/82 ,  C12N15/87 ,  C11B1/00

CPC分类号： C12N15/8243 ,  C12N15/8218 ,  C12N15/8285 ,  C12P7/62 ,  C12P13/02

摘要： The present invention provides a genetically modified plant that biosynthesizes an increased amount of capsinoids, a method of producing the genetically modified plant, and a production method of capsinoids from the genetically modified plant. More particularly, the present invention provides a genetically modified plant capable of producing capsinoids, which shows a decreased expression or activity of an enzyme that catalyzes an amino group conversion reaction from vanillin to vanillylamine as compared to wild strains, and the like. As a method of suppressing expression or activity of an enzyme, an introduction of DNA encoding an antisense RNA, iRNA, ribozyme or dominant-negative mutant for the target gene, a destruction of the gene by a knockout method, mutagen treatment or transposon insertion, an introduction of a gene encoding an antibody against the enzyme and the like can be mentioned.

摘要翻译： 本发明提供了生物合成增加量的类毒素的遗传修饰植物，生物转基因植物的方法以及来自转基因植物的类毒素的制备方法。 更具体地，本发明提供能够产生辣椒素的遗传修饰植物，与野生株相比，其表现出从香草醛向香草胺催化氨基转化反应的酶的表达或活性降低。 作为抑制酶的表达或活性的方法，引入编码靶基因的反义RNA，iRNA，核酶或显性失活突变体的DNA，通过敲除法，诱变处理或转座子插入破坏基因， 可以提及编码针对酶等的抗体的基因的引入。