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    Methods of producing nucleic acids coding for proteins have raffinose synthase activity
    2.
    发明申请
    Methods of producing nucleic acids coding for proteins have raffinose synthase activity 审中-公开
    编码蛋白质的核酸的方法具有棉子糖合酶活性

    公开(公告)号：US20050066391A1

    公开(公告)日：2005-03-24

    申请号：US10948251

    申请日：2004-09-24

    申请人： Chieko Osumi ,  Jinshi Nozaki ,  Takao Kida

    发明人： Chieko Osumi ,  Jinshi Nozaki ,  Takao Kida

    IPC分类号： C12N9/10 ,  C12N15/82 ,  A01H1/00 ,  C07H21/04 ,  C12N5/04 ,  C12N9/24

    CPC分类号： C12N9/1051 ,  C12N15/8245 ,  C12N15/8246

    摘要： Raffinose is produced by allowing a raffinose synthase having the following properties to act on sucrose and galactinol: (1) action and substrate specificity: produces raffinose from sucrose and galactinol; (2) optimum pH: about 6 to 8; (3) optimum temperature: about 35 to 40° C.; (4) molecular weight: (i) about 75 kDa to 95 kDa estimated by gel filtration chromatography; (ii) about 90 kDa to 100 kDa estimated by polyacrylamide gel electrophoresis (Native PAGE); and (iii) about 90 kDa to 100 kDa estimated by SDS-polyacrylamide gel electrophoresis under a reduced condition (SDS-PAGE); and (5) inhibition: inhibited by iodoacetamide, N-ethylmaleimide, and myo-inositol.

    摘要翻译： 通过使具有以下性质的棉子糖合成酶作用于蔗糖和半乳聚糖来制备棉子糖：（1）作用和底物特异性：从蔗糖和肌醇半乳糖苷产生棉子糖; （2）最适pH：约6〜8; （3）最适温度：约35〜40℃。 （4）分子量：（i）通过凝胶过滤色谱法估计约75kDa至95kDa; （ii）通过聚丙烯酰胺凝胶电泳（Native PAGE）估计约90kDa至100kDa; 和（iii）在还原条件（SDS-PAGE）下通过SDS-聚丙烯酰胺凝胶电泳估计约90kDa至100kDa; 和（5）抑制：被碘乙酰胺，N-乙基马来酰亚胺和肌醇肌醇抑制。

    Soybean raffinose synthase and a method for producing raffinose
    3.
    发明授权
    Soybean raffinose synthase and a method for producing raffinose 失效
    大豆棉子糖合成酶和生产棉子糖的方法

    公开(公告)号：US07455996B2

    公开(公告)日：2008-11-25

    申请号：US11034848

    申请日：2005-01-14

    申请人： Chieko Osumi ,  Jinshi Nozaki ,  Takao Kida

    发明人： Chieko Osumi ,  Jinshi Nozaki ,  Takao Kida

    IPC分类号： C12P19/18 ,  C12N1/20 ,  C12N9/10 ,  C07H21/04

    CPC分类号： C12N9/1051 ,  C12N15/8245 ,  C12N15/8246

    摘要： Raffinose is produced by allowing a raffinose synthase having the following properties to act on sucrose and galactinol: (1) action and substrate specificity: produces raffinose from sucrose and galactinol; (2) optimum pH: about 6 to 8; (3) optimum temperature: about 35 to 40° C.; (4) molecular weight: (i) about 75 kDa to 95 kDa estimated by gel filtration chromatography; (ii) about 90 kDa to 100 kDa estimated by polyacrylamide gel electrophoresis (Native PAGE); and (iii) about 90 kDa to 100 kDa estimated by SDS-polyacrylamide gel electrophoresis under a reduced condition (SDS-PAGE); and (5) inhibition: inhibited by iodoacetamide, N-ethylmaleimide, and myo-inositol.

    摘要翻译： 通过使具有以下性质的棉子糖合成酶作用于蔗糖和半乳聚糖来制备棉子糖：（1）作用和底物特异性：从蔗糖和肌醇半乳糖苷产生棉子糖; （2）最适pH：约6〜8; （3）最适温度：约35〜40℃。 （4）分子量：（i）通过凝胶过滤色谱法估计约75kDa至95kDa; （ii）通过聚丙烯酰胺凝胶电泳（Native PAGE）估计约90kDa至100kDa; 和（iii）在还原条件（SDS-PAGE）下通过SDS-聚丙烯酰胺凝胶电泳估计约90kDa至100kDa; 和（5）抑制：被碘乙酰胺，N-乙基马来酰亚胺和肌醇肌醇抑制。

    Soybean raffinose synthase and a method for producing raffinose
    4.
    发明申请
    Soybean raffinose synthase and a method for producing raffinose 失效
    大豆棉子糖合成酶和生产棉子糖的方法

    公开(公告)号：US20050160497A1

    公开(公告)日：2005-07-21

    申请号：US11034678

    申请日：2005-01-14

    申请人： Chieko Osumi ,  Jinshi Nozaki ,  Takao Kida

    发明人： Chieko Osumi ,  Jinshi Nozaki ,  Takao Kida

    IPC分类号： C12N9/10 ,  C12N15/82 ,  A01H1/00 ,  C07H21/04 ,  C12N5/04

    CPC分类号： C12N9/1051 ,  C12N15/8245 ,  C12N15/8246

    摘要： Raffinose is produced by allowing a raffinose synthase having the following properties to act on sucrose and galactinol: (1) action and substrate specificity: produces raffinose from sucrose and galactinol; (2) optimum pH: about 6 to 8; (3) optimum temperature: about 35 to 40° C.; (4) molecular weight: (i) about 75 kDa to 95 kDa estimated by gel filtration chromatography; (ii) about 90 kDa to 100 kDa estimated by polyacrylamide gel electrophoresis (Native PAGE); and (iii) about 90 kDa to 100 kDa estimated by SDS-polyacrylamide gel electrophoresis under a reduced condition (SDS-PAGE); and (5) inhibition: inhibited by iodoacetamide, N-ethylmaleimide, and myo-inositol.

    摘要翻译： 通过使具有以下性质的棉子糖合成酶作用于蔗糖和半乳聚糖来制备棉子糖：（1）作用和底物特异性：从蔗糖和肌醇半乳糖苷产生棉子糖; （2）最适pH：约6〜8; （3）最适温度：约35〜40℃。 （4）分子量：（i）通过凝胶过滤色谱法估计约75kDa至95kDa; （ii）通过聚丙烯酰胺凝胶电泳（Native PAGE）估计约90kDa至100kDa; 和（iii）在还原条件（SDS-PAGE）下通过SDS-聚丙烯酰胺凝胶电泳估计约90kDa至100kDa; 和（5）抑制：被碘乙酰胺，N-乙基马来酰亚胺和肌醇肌醇抑制。

    METHOD OF PRODUCING TRANSGENIC PLANTS HAVING IMPROVED AMINO ACID COMPOSITION AND IMPROVED YIELDING
    8.
    发明申请
    METHOD OF PRODUCING TRANSGENIC PLANTS HAVING IMPROVED AMINO ACID COMPOSITION AND IMPROVED YIELDING 审中-公开
    生产具有改进的氨基酸组成的转基因植物的方法和改进的接种

    公开(公告)号：US20070130643A1

    公开(公告)日：2007-06-07

    申请号：US11626131

    申请日：2007-01-23

    申请人： Hiroaki KISAKA ,  Takao Kida

    发明人： Hiroaki KISAKA ,  Takao Kida

    IPC分类号： A01H5/00 ,  A01H1/00

    CPC分类号： C12N9/0004 ,  C12N9/0016 ,  C12N9/93 ,  C12N15/8241 ,  C12N15/8251 ,  C12N15/8261 ,  C12Y104/01002 ,  C12Y104/01004 ,  Y02A40/146

    摘要： The object of the present invention is to provide transgenic plants which accumulate free amino acids, particularly at least one amino acid selected from among glutamic acid, asparagine, aspartic acid, serine, threonine, alanine and histidine accumulated in a large amount, in the edible parts thereof, and a method of producing them are provided. The other object of the present invention is to provide a method of increasing the yielding of potato and to provide a transgenic potato of which yielding can be increased. In the present invention, a sequence encoding glutamate dehydrogenase (GDH) gene is introduced into a plant together with a suitable regulatory sequence to express it in a plant cell, and thereby the GDH gene is excessively expressed.

    摘要翻译： 本发明的目的是提供一种转基因植物，其在食用中积累游离氨基酸，特别是至少一种选自谷氨酸，天冬酰胺，天冬氨酸，丝氨酸，苏氨酸，丙氨酸和组氨酸中的至少一种氨基酸 部分，以及它们的制造方法。 本发明的另一个目的是提供一种增加马铃薯产量的方法，并且提供可以提高产量的转基因马铃薯。 在本发明中，将编码谷氨酸脱氢酶（GDH）基因的序列与合适的调节序列一起导入植物，以在植物细胞中表达，从而过度表达GDH基因。