公开(公告)号：US20050114925A1

公开(公告)日：2005-05-26

申请号：US10970987

申请日：2004-10-25

申请人： Hiroaki Kisaka ,  Shuichi Yanagisawa ,  Tetsuya Miwa ,  Ai Akiyama

发明人： Hiroaki Kisaka ,  Shuichi Yanagisawa ,  Tetsuya Miwa ,  Ai Akiyama

IPC分类号： A01H5/00 ,  C08B30/04 ,  C12N15/09 ,  C12N15/29 ,  C12N15/82 ,  A01H1/00

CPC分类号： C08B30/048 ,  C12N15/8245 ,  C12N15/8261 ,  Y02A40/146

摘要： The present invention provides potatoes having a tuber yield of potatoes per plant higher than that of non-transformed potatoes cultivated under the same conditions, wherein a gene encoding DNA linking protein belonging to Dof family is introduced in the potatoes. The present invention also provides a method for producing potatoes having a tuber yield of potatoes per plant body higher than that of non-transformed potatoes cultivated under the same conditions, comprising introducing a gene encoding a DNA linking protein belonging to Dof family into the potatoes, and expressing said gene in the potatoes.

摘要翻译： 本发明提供了在相同条件下培养的具有高于非转化马铃薯的植物的马铃薯块茎产量的马铃薯，其中将编码属于Dof家族的DNA连接蛋白质的基因导入马铃薯中。 本发明还提供了一种生产马铃薯的方法，所述土豆具有比在相同条件下培养的非转化马铃薯高的马铃薯的每株植物的块茎产量，包括将编码属于Dof家族的DNA连接蛋白的基因导入马铃薯， 并在马铃薯中表达所述基因。

公开(公告)号：US20110166371A1

公开(公告)日：2011-07-07

申请号：US12976526

申请日：2010-12-22

申请人： Hiroaki KISAKA ,  Yaqin Lang ,  Ryuji Sugiyama ,  Tetsuya Miwa ,  Susumu Yazawa

发明人： Hiroaki KISAKA ,  Yaqin Lang ,  Ryuji Sugiyama ,  Tetsuya Miwa ,  Susumu Yazawa

IPC分类号： A01H5/00 ,  C12N15/82 ,  C12N15/87 ,  C11B1/00

CPC分类号： C12N15/8243 ,  C12N15/8218 ,  C12N15/8285 ,  C12P7/62 ,  C12P13/02

摘要： The present invention provides a genetically modified plant that biosynthesizes an increased amount of capsinoids, a method of producing the genetically modified plant, and a production method of capsinoids from the genetically modified plant. More particularly, the present invention provides a genetically modified plant capable of producing capsinoids, which shows a decreased expression or activity of an enzyme that catalyzes an amino group conversion reaction from vanillin to vanillylamine as compared to wild strains, and the like. As a method of suppressing expression or activity of an enzyme, an introduction of DNA encoding an antisense RNA, iRNA, ribozyme or dominant-negative mutant for the target gene, a destruction of the gene by a knockout method, mutagen treatment or transposon insertion, an introduction of a gene encoding an antibody against the enzyme and the like can be mentioned.

摘要翻译： 本发明提供了生物合成增加量的类毒素的遗传修饰植物，生物转基因植物的方法以及来自转基因植物的类毒素的制备方法。 更具体地，本发明提供能够产生辣椒素的遗传修饰植物，与野生株相比，其表现出从香草醛向香草胺催化氨基转化反应的酶的表达或活性降低。 作为抑制酶的表达或活性的方法，引入编码靶基因的反义RNA，iRNA，核酶或显性失活突变体的DNA，通过敲除法，诱变处理或转座子插入破坏基因， 可以提及编码针对酶等的抗体的基因的引入。

公开(公告)号：US06969782B2

公开(公告)日：2005-11-29

申请号：US09729821

申请日：2000-12-06

申请人： Hiroaki Kisaka ,  Takao Kida

发明人： Hiroaki Kisaka ,  Takao Kida

IPC分类号： C12N9/00 ,  C12N9/02 ,  C12N15/53 ,  C12N15/82 ,  C12N15/83 ,  C12N15/90 ,  A01H5/00 ,  A01H5/10

CPC分类号： C12N9/0004 ,  C12N9/0016 ,  C12N9/93 ,  C12N15/8241 ,  C12N15/8251 ,  C12N15/8261 ,  C12Y104/01002 ,  C12Y104/01004 ,  Y02A40/146

摘要： Transgenic plants containing free amino acids, particularly at least one amino acid selected from among glutamic acid, asparagine, aspartic acid, serine, threonine, alanine and histidine accumulated in a large amount, in edible parts thereof, and a method of producing them are provided. In this method, glutamate dehydrogenase (GDH) gene is introduced into a plant together with a regulator sequence suitable for over expressing the sequence encoding GDH gene in plant cells.

摘要翻译： 提供含有游离氨基酸，特别是选自谷氨酸，天冬酰胺，天冬氨酸，丝氨酸，苏氨酸，丙氨酸和组氨酸中的至少一种氨基酸的大量植物，其可食用部分的转基因植物及其制备方法 。 在该方法中，将谷氨酸脱氢酶（GDH）基因与适于过度表达植物细胞中编码GDH基因的序列的调节序列一起引入植物。

公开(公告)号：US20050183168A1

公开(公告)日：2005-08-18

申请号：US11059687

申请日：2005-02-17

申请人： Hiroaki Kisaka ,  Tetsuya Miwa

发明人： Hiroaki Kisaka ,  Tetsuya Miwa

IPC分类号： A01H5/00 ,  A01H1/00 ,  C12N5/10 ,  C12N9/06 ,  C12N15/09 ,  C12N15/29 ,  C12N15/82

CPC分类号： C12N15/8267 ,  C12N9/0016 ,  C12N15/8261 ,  Y02A40/146

摘要： The present invention provides a plant having a decreased dormancy period, a plant having a decreased dormancy period and uniform budding and/or germination timing, and a method for producing these plants. The intracellular 2-OG content in the plant may be increased, for example, by overexpressing an intracellular GDH gene in the plant. More specifically, the intracellular 2-OG content in the plant may be increased due to the overexpression of the GDH gene, by introducing into the plant a nucleic acid construct capable of enhancing expression of the GDH gene, particularly a nucleic acid construct capable of expressing the GDH gene.

摘要翻译： 本发明提供了具有降低的休眠期的植物，具有降低的休眠期和均匀的芽和/或发芽时间的植物以及这些植物的生产方法。 植物中的细胞内2-OG含量可以增加，例如通过过表达植物中的细胞内GDH基因。 更具体地说，由于GDH基因的过度表达，植物中的细胞内2-OG含量可以通过向植物中引入能够增强GDH基因表达的核酸构建体，特别是能够表达GDH基因的核酸构建体 GDH基因。

公开(公告)号：US06727411B2

公开(公告)日：2004-04-27

申请号：US09727758

申请日：2000-12-04

申请人： Hiroaki Kisaka ,  Takao Kida

发明人： Hiroaki Kisaka ,  Takao Kida

IPC分类号： C12N1529

CPC分类号： C12N9/88 ,  C12N15/8251 ,  C12N15/8253

摘要： Transgenic plants containing free amino acids, particularly at least one amino acid selected from among glutamic acid, asparagine, aspartic acid, serine, threonine, alanine and histidine accumulated in a large amount, in edible parts thereof, and a method of producing them are provided. In this method, glutamate decarboxylase(GAD) gene is introduced into a plant in anti-sense direction together with an appropriate regulatory sequence, and the expression of GAD gene is supressed.

摘要翻译： 提供含有游离氨基酸，特别是选自谷氨酸，天冬酰胺，天冬氨酸，丝氨酸，苏氨酸，丙氨酸和组氨酸中的至少一种氨基酸的大量植物，其可食用部分的转基因植物及其制备方法 在该方法中，将谷氨酸脱羧酶（GAD）基因连同适当的调节序列一起引入反义方向的植物中，并且抑制GAD基因的表达。

公开(公告)号：US09340795B2

公开(公告)日：2016-05-17

申请号：US12976526

申请日：2010-12-22

申请人： Hiroaki Kisaka ,  Yaqin Lang ,  Ryuji Sugiyama ,  Tetsuya Miwa ,  Susumu Yazawa

发明人： Hiroaki Kisaka ,  Yaqin Lang ,  Ryuji Sugiyama ,  Tetsuya Miwa ,  Susumu Yazawa

IPC分类号： C12N15/82 ,  C12N15/87 ,  C12P7/62 ,  C12P13/02

CPC分类号： C12N15/8243 ,  C12N15/8218 ,  C12N15/8285 ,  C12P7/62 ,  C12P13/02

摘要： The present invention provides a genetically modified plant that biosynthesizes an increased amount of capsinoids, a method of producing the genetically modified plant, and a production method of capsinoids from the genetically modified plant. More particularly, the present invention provides a genetically modified plant capable of producing capsinoids, which shows a decreased expression or activity of an enzyme that catalyzes an amino group conversion reaction from vanillin to vanillylamine as compared to wild strains, and the like. As a method of suppressing expression or activity of an enzyme, an introduction of DNA encoding an antisense RNA, iRNA, ribozyme or dominant-negative mutant for the target gene, a destruction of the gene by a knockout method, mutagen treatment or transposon insertion, an introduction of a gene encoding an antibody against the enzyme and the like can be mentioned.

摘要翻译： 本发明提供了生物合成增加量的类毒素的遗传修饰植物，生物转基因植物的方法以及来自转基因植物的类毒素的制备方法。 更具体地，本发明提供能够产生辣椒素的遗传修饰植物，与野生株相比，其表现出从香草醛向香草胺催化氨基转化反应的酶的表达或活性降低。 作为抑制酶的表达或活性的方法，引入编码靶基因的反义RNA，iRNA，核酶或显性失活突变体的DNA，通过敲除法，诱变处理或转座子插入破坏基因， 可以提及编码针对酶等的抗体的基因的引入。

公开(公告)号：US07569388B2

公开(公告)日：2009-08-04

申请号：US10360634

申请日：2003-02-10

申请人： Shuichi Yanagisawa ,  Tetsuya Miwa ,  Ai Akiyama ,  Hiroaki Kisaka

发明人： Shuichi Yanagisawa ,  Tetsuya Miwa ,  Ai Akiyama ,  Hiroaki Kisaka

IPC分类号： A01H1/00 ,  C07H21/04 ,  C07K14/415 ,  C12N15/00

CPC分类号： C07K14/415 ,  C12N15/8251

摘要： The object of the present invention is to provide a transformed plant having an enhanced amino acid content, a progeny of the plant, seeds of the plant and a method for producing the plant. The present invention provides a transformed plant having a gene encoding DNA binding protein of Dof family and also having a free amino acid content higher than that of naturally occurring plants of the same species cultivated under the same conditions. The present invention also provides a method of producing a transformed plant having the free amino acid content higher than that of naturally occurring plants of the same species cultivated under the same conditions by introducing a gene encoding DNA binding protein of Dof family into the plant to express the gene in the plant body.

摘要翻译： 本发明的目的是提供具有增强的氨基酸含量的转化植物，植物的后代，植物的种子和用于生产植物的方法。 本发明提供了具有编码Dof家族的DNA结合蛋白的基因的转化植物，并且其游离氨基酸含量高于在相同条件下栽培的相同种类的天然植物的游离氨基酸含量。 本发明还提供了通过将编码Dof家族的DNA结合蛋白的基因导入植物来表达在相同条件下培养的相同种类的天然植物的游离氨基酸含量高的转化植物的生产方法，以表达 植物体内的基因。

公开(公告)号：US20060090219A1

公开(公告)日：2006-04-27

申请号：US11302262

申请日：2005-12-14

申请人： Hiroaki Kisaka ,  Tetsuya Miwa ,  Ai Akiyama

发明人： Hiroaki Kisaka ,  Tetsuya Miwa ,  Ai Akiyama

IPC分类号： A01H1/00 ,  C12N15/82

CPC分类号： C12N15/8261 ,  Y02A40/146

摘要： The present invention provides a method of producing a plant which exhibits improved growth and/or yield under reduced nitrogen conditions, that is, under cultivation conditions where nitrogen is limited as compared to ordinary cultivation conditions, by increasing 2-OG content in plants. Particularly, the present invention provides a method of producing a plant which exhibits improved growth and/or yield under conditions where nitrogen is reduced, that is, under cultivation conditions where nitrogen is limited as compared to ordinary cultivation conditions, by introducing a GDH gene or ECASPC gene into plants and expressing the transgene GDH or ECAPS in the plants, which results in increased 2-OG, or by spraying proline on the leaves of plants to increase the 2-OG content, thereby enhancing the incorporation of nitrogen or metabolic activity of plants. Also provided is a method of cultivating such plants under nitrogen-limited conditions.

摘要翻译： 本发明提供一种生产植物的方法，其在减少的氮条件下，即通过增加植物中2-OG含量，在与普通培养条件相比限制氮的培养条件下，其生长和/或产量改善。 特别地，本发明提供一种生产植物的方法，该方法在氮的还原条件下，即在与普通培养条件相比限制的培养条件下，通过引入GDH基因或 ECASPC基因植入植物中并在植物中表达转基因GDH或ECAPS，其导致增加的2-OG，或通过在植物叶上喷洒脯氨酸以增加2-OG含量，从而增强氮的掺入或代谢活性 植物。 还提供了在氮限制条件下培养这样的植物的方法。

公开(公告)号：US20070130643A1

公开(公告)日：2007-06-07

申请号：US11626131

申请日：2007-01-23

申请人： Hiroaki KISAKA ,  Takao Kida

发明人： Hiroaki KISAKA ,  Takao Kida

IPC分类号： A01H5/00 ,  A01H1/00

CPC分类号： C12N9/0004 ,  C12N9/0016 ,  C12N9/93 ,  C12N15/8241 ,  C12N15/8251 ,  C12N15/8261 ,  C12Y104/01002 ,  C12Y104/01004 ,  Y02A40/146

摘要： The object of the present invention is to provide transgenic plants which accumulate free amino acids, particularly at least one amino acid selected from among glutamic acid, asparagine, aspartic acid, serine, threonine, alanine and histidine accumulated in a large amount, in the edible parts thereof, and a method of producing them are provided. The other object of the present invention is to provide a method of increasing the yielding of potato and to provide a transgenic potato of which yielding can be increased. In the present invention, a sequence encoding glutamate dehydrogenase (GDH) gene is introduced into a plant together with a suitable regulatory sequence to express it in a plant cell, and thereby the GDH gene is excessively expressed.

摘要翻译： 本发明的目的是提供一种转基因植物，其在食用中积累游离氨基酸，特别是至少一种选自谷氨酸，天冬酰胺，天冬氨酸，丝氨酸，苏氨酸，丙氨酸和组氨酸中的至少一种氨基酸 部分，以及它们的制造方法。 本发明的另一个目的是提供一种增加马铃薯产量的方法，并且提供可以提高产量的转基因马铃薯。 在本发明中，将编码谷氨酸脱氢酶（GDH）基因的序列与合适的调节序列一起导入植物，以在植物细胞中表达，从而过度表达GDH基因。

公开(公告)号：US07176351B2

公开(公告)日：2007-02-13

申请号：US10970987

申请日：2004-10-25

申请人： Hiroaki Kisaka ,  Shuichi Yanagisawa ,  Tetsuya Miwa ,  Ai Akiyama

发明人： Hiroaki Kisaka ,  Shuichi Yanagisawa ,  Tetsuya Miwa ,  Ai Akiyama

IPC分类号： A01H5/00 ,  C12N5/04 ,  C12N15/29 ,  C12N15/82

CPC分类号： C08B30/048 ,  C12N15/8245 ,  C12N15/8261 ,  Y02A40/146

摘要： The present invention provides potatoes having a tuber yield of potatoes per plant higher than that of non-transformed potatoes cultivated under the same conditions, wherein a gene encoding DNA linking protein belonging to Dof family is introduced in the potatoes. The present invention also provides a method for producing potatoes having a tuber yield of potatoes per plant body higher than that of non-transformed potatoes cultivated under the same conditions, comprising introducing a gene encoding a DNA linking protein belonging to Dof family into the potatoes, and expressing said gene in the potatoes.

摘要翻译： 本发明提供了在相同条件下培养的具有高于非转化马铃薯的植物的马铃薯块茎产量的马铃薯，其中将编码属于Dof家族的DNA连接蛋白质的基因导入马铃薯中。 本发明还提供了一种生产马铃薯的方法，所述土豆具有比在相同条件下培养的非转化马铃薯高的马铃薯的每株植物的块茎产量，包括将编码属于Dof家族的DNA连接蛋白的基因导入马铃薯， 并在马铃薯中表达所述基因。