公开(公告)号：US20050114925A1

公开(公告)日：2005-05-26

申请号：US10970987

申请日：2004-10-25

申请人： Hiroaki Kisaka ,  Shuichi Yanagisawa ,  Tetsuya Miwa ,  Ai Akiyama

发明人： Hiroaki Kisaka ,  Shuichi Yanagisawa ,  Tetsuya Miwa ,  Ai Akiyama

IPC分类号： A01H5/00 ,  C08B30/04 ,  C12N15/09 ,  C12N15/29 ,  C12N15/82 ,  A01H1/00

CPC分类号： C08B30/048 ,  C12N15/8245 ,  C12N15/8261 ,  Y02A40/146

摘要： The present invention provides potatoes having a tuber yield of potatoes per plant higher than that of non-transformed potatoes cultivated under the same conditions, wherein a gene encoding DNA linking protein belonging to Dof family is introduced in the potatoes. The present invention also provides a method for producing potatoes having a tuber yield of potatoes per plant body higher than that of non-transformed potatoes cultivated under the same conditions, comprising introducing a gene encoding a DNA linking protein belonging to Dof family into the potatoes, and expressing said gene in the potatoes.

摘要翻译： 本发明提供了在相同条件下培养的具有高于非转化马铃薯的植物的马铃薯块茎产量的马铃薯，其中将编码属于Dof家族的DNA连接蛋白质的基因导入马铃薯中。 本发明还提供了一种生产马铃薯的方法，所述土豆具有比在相同条件下培养的非转化马铃薯高的马铃薯的每株植物的块茎产量，包括将编码属于Dof家族的DNA连接蛋白的基因导入马铃薯， 并在马铃薯中表达所述基因。

公开(公告)号：US07176351B2

公开(公告)日：2007-02-13

申请号：US10970987

申请日：2004-10-25

申请人： Hiroaki Kisaka ,  Shuichi Yanagisawa ,  Tetsuya Miwa ,  Ai Akiyama

发明人： Hiroaki Kisaka ,  Shuichi Yanagisawa ,  Tetsuya Miwa ,  Ai Akiyama

IPC分类号： A01H5/00 ,  C12N5/04 ,  C12N15/29 ,  C12N15/82

CPC分类号： C08B30/048 ,  C12N15/8245 ,  C12N15/8261 ,  Y02A40/146

摘要： The present invention provides potatoes having a tuber yield of potatoes per plant higher than that of non-transformed potatoes cultivated under the same conditions, wherein a gene encoding DNA linking protein belonging to Dof family is introduced in the potatoes. The present invention also provides a method for producing potatoes having a tuber yield of potatoes per plant body higher than that of non-transformed potatoes cultivated under the same conditions, comprising introducing a gene encoding a DNA linking protein belonging to Dof family into the potatoes, and expressing said gene in the potatoes.

摘要翻译： 本发明提供了在相同条件下培养的具有高于非转化马铃薯的植物的马铃薯块茎产量的马铃薯，其中将编码属于Dof家族的DNA连接蛋白质的基因导入马铃薯中。 本发明还提供了一种生产马铃薯的方法，所述土豆具有比在相同条件下培养的非转化马铃薯高的马铃薯的每株植物的块茎产量，包括将编码属于Dof家族的DNA连接蛋白的基因导入马铃薯， 并在马铃薯中表达所述基因。

公开(公告)号：US07569388B2

公开(公告)日：2009-08-04

申请号：US10360634

申请日：2003-02-10

申请人： Shuichi Yanagisawa ,  Tetsuya Miwa ,  Ai Akiyama ,  Hiroaki Kisaka

发明人： Shuichi Yanagisawa ,  Tetsuya Miwa ,  Ai Akiyama ,  Hiroaki Kisaka

IPC分类号： A01H1/00 ,  C07H21/04 ,  C07K14/415 ,  C12N15/00

CPC分类号： C07K14/415 ,  C12N15/8251

摘要： The object of the present invention is to provide a transformed plant having an enhanced amino acid content, a progeny of the plant, seeds of the plant and a method for producing the plant. The present invention provides a transformed plant having a gene encoding DNA binding protein of Dof family and also having a free amino acid content higher than that of naturally occurring plants of the same species cultivated under the same conditions. The present invention also provides a method of producing a transformed plant having the free amino acid content higher than that of naturally occurring plants of the same species cultivated under the same conditions by introducing a gene encoding DNA binding protein of Dof family into the plant to express the gene in the plant body.

摘要翻译： 本发明的目的是提供具有增强的氨基酸含量的转化植物，植物的后代，植物的种子和用于生产植物的方法。 本发明提供了具有编码Dof家族的DNA结合蛋白的基因的转化植物，并且其游离氨基酸含量高于在相同条件下栽培的相同种类的天然植物的游离氨基酸含量。 本发明还提供了通过将编码Dof家族的DNA结合蛋白的基因导入植物来表达在相同条件下培养的相同种类的天然植物的游离氨基酸含量高的转化植物的生产方法，以表达 植物体内的基因。

公开(公告)号：US20060090219A1

公开(公告)日：2006-04-27

申请号：US11302262

申请日：2005-12-14

申请人： Hiroaki Kisaka ,  Tetsuya Miwa ,  Ai Akiyama

发明人： Hiroaki Kisaka ,  Tetsuya Miwa ,  Ai Akiyama

IPC分类号： A01H1/00 ,  C12N15/82

CPC分类号： C12N15/8261 ,  Y02A40/146

摘要： The present invention provides a method of producing a plant which exhibits improved growth and/or yield under reduced nitrogen conditions, that is, under cultivation conditions where nitrogen is limited as compared to ordinary cultivation conditions, by increasing 2-OG content in plants. Particularly, the present invention provides a method of producing a plant which exhibits improved growth and/or yield under conditions where nitrogen is reduced, that is, under cultivation conditions where nitrogen is limited as compared to ordinary cultivation conditions, by introducing a GDH gene or ECASPC gene into plants and expressing the transgene GDH or ECAPS in the plants, which results in increased 2-OG, or by spraying proline on the leaves of plants to increase the 2-OG content, thereby enhancing the incorporation of nitrogen or metabolic activity of plants. Also provided is a method of cultivating such plants under nitrogen-limited conditions.

摘要翻译： 本发明提供一种生产植物的方法，其在减少的氮条件下，即通过增加植物中2-OG含量，在与普通培养条件相比限制氮的培养条件下，其生长和/或产量改善。 特别地，本发明提供一种生产植物的方法，该方法在氮的还原条件下，即在与普通培养条件相比限制的培养条件下，通过引入GDH基因或 ECASPC基因植入植物中并在植物中表达转基因GDH或ECAPS，其导致增加的2-OG，或通过在植物叶上喷洒脯氨酸以增加2-OG含量，从而增强氮的掺入或代谢活性 植物。 还提供了在氮限制条件下培养这样的植物的方法。

公开(公告)号：US06456446B1

公开(公告)日：2002-09-24

申请号：US09688918

申请日：2000-10-16

申请人： Ai Akiyama ,  Tamotsu Koiwai

发明人： Ai Akiyama ,  Tamotsu Koiwai

IPC分类号： G02B702

CPC分类号： G02B7/026

摘要： A lens holding frame holding a lens having a circular outer diameter includes a first holding member forming an inscribed circle corresponding to the outer circumference of the lens and a second holding member forming a larger inscribed circle than the inscribed circle formed by the first holding member. The first holding member comprises three projections and they are located at an equal distance in the circumferential direction of the lens holding frame. Similarly, the second holding member also comprises three projections and they are located at an equal distance in the circumferential direction of the lens holding frame. In this case, the projections forming the first holding member and the projections forming the second holding member are located alternately on the inner diameter of the lens holding frame in the circumferential direction respectively. Further, the projections composing the second holding member are composed so that they will form a larger inscribed circle than the inscribed circle formed by the projections composing the first holding member.

摘要翻译： 保持具有圆形外径的透镜的透镜保持框架包括形成与透镜的外周相对应的内切圆的第一保持构件和形成比由第一保持构件形成的内切圆更大的内接圆的第二保持构件。 第一保持构件包括三个突起，并且它们在透镜保持框架的圆周方向上以相等的距离定位。 类似地，第二保持构件还包括三个突起，并且它们在透镜保持框架的圆周方向上位于相等的距离处。 在这种情况下，形成第一保持构件的突起和形成第二保持构件的突起分别在透镜保持框的内径上分别交替设置。 此外，构成第二保持构件的突起构成为与由构成第一保持构件的突起形成的内接圆形成更大的内接圆。